flow cytometry

The paper identifies T1LN as a glycan epitope specific to human pluripotent stem cells (hPSCs) that is rapidly downregulated during differentiation, enabling its use for immunodepletion of hPSCs from differentiated cell populations.

The paper tracks anti-viral CD8+ T cells to show lymphatic transit seeds LN TRM, which provide protection against rechallenge.

The paper identifies a new subset of tonsillar memory B cells that serve as precursors for IgD-secreting plasma cells, contributing to steady-state IgD responses to common antigens.

This study shows that heterologous immunization primes antigen-specific memory CD4 T cells, enhancing early germinal center response but not antibody generation in influenza infection.

This study identifies a specific immune profile in cystic fibrosis patients linked to lung microbiota and immune dysregulation, offering potential targets for treatment.

This study shows that sepiapterin reprograms M2-type tumor-associated macrophages in HER2+ breast tumors to M1-type by modulating arginine metabolism, enhancing immunotherapy response.

This study demonstrates that IL-10-producing group 2 innate lymphoid cells (ILC210) can suppress Graft-versus-Host Disease (GVHD) in a humanized model without compromising graft-versus-leukemic effects.

The paper compares thymocyte and splenocyte migration between lymphoid organs in pregnant and non-pregnant mice, finding similar mobility but suggesting spleen and lymph node enlargement may result from leukocyte retention.

This paper introduces the CytoBas assay, a flow cytometry method using fluorescent allergen tetramers to detect HDM sensitization in basophils, showing high sensitivity and specificity for Der p 1 and Der p 2.

The paper analyzes tissue-resident regulatory T cells (Tregs) in non-lymphoid organs, showing they share phenotypes, transient residency, and TCR sequences, with tissue-agnostic re-entry capabilities.

This study shows cryopreserved monocytes from HSCT donors can generate regulatory dendritic cells (DCreg) with similar phenotypes and cytokine profiles to fresh cells, supporting their use in GVHD treatment.

The paper presents standardized flow cytometry methods for identifying and isolating ctenophore phagocytes, enabling detailed analysis of cellular processes in this early-diverging metazoan group.

This study describes an all-stages complete suspension protocol for generating functional pancreatic islets from human pluripotent stem cells using Vertical Wheel bioreactors.

This study examines how ageing and frailty affect monocyte and dendritic cell subsets in blood using flow cytometry in an immune ageing cohort.

The paper finds that higher levels of CD4+ T cell senescence in severe/critical COVID-19 patients correlate with reduced antibody responses and worse survival, especially in breakthrough infections.

This study identifies T cell signatures associated with reduced Chlamydia trachomatis reinfection in a highly exposed cohort using mass cytometry and random forest analysis.

This paper develops a deep learning model for detecting rare circulating tumor cell clusters in whole blood using label-free flow cytometry, achieving high correlation and low false alarm rates.

This study shows NK cells lyse P. falciparum-infected erythrocytes via ADCC, releasing parasitophorous vacuoles that are phagocytosed by monocytes in the presence of immune IgG.

The paper identifies profibrotic monocyte-derived alveolar macrophages and neutrophil recruitment as drivers of lung fibrosis in patients with persistent respiratory symptoms and radiographic abnormalities after COVID-19.

The paper identifies distinct FAP subpopulations in human musculoskeletal injuries that drive adipogenesis and fibrosis, showing that DLK1 downregulation promotes degeneration.

This paper introduces an unbiased method to identify and account for heterogeneous autofluorescence spectra in flow cytometry data, improving unmixing accuracy across diverse biological samples.

This paper presents a 43-parameter flow cytometry panel for analyzing peripheral blood mononuclear cells, demonstrating improved resolution and reproducibility through spectral technology and optimized experimental design.

This study uses AI with flow cytometry to identify CML cells with mitochondrial morphological changes, enabling early diagnosis and monitoring of treatment response.

This paper shows TRPV1 regulates immune responses to Citrobacter rodentium infection by modulating neutrophil recruitment through chemokine and adhesion molecule expression.

This study shows that deleting Vhl in Dmp1-expressing cells increases stress erythropoiesis by altering MEP metabolism and inducing hypoxia-like responses.

This study shows IL-15 treatment enhances the anti-tumor efficacy of sipuleucel-T by increasing CD8+ T-cell and CD56+ NKT subset activity in prostate cancer.

This study compares the effects of different disease-modifying therapies on immune cell profiles in relapsing-remitting multiple sclerosis patients, finding varied impacts on T, B, and NK cells.

This study investigates cytokine polyfunctionality dynamics in PBMCs using a single-cell microfluidic platform, revealing distinct secretion patterns and reduced polyfunctionality with prolonged stimulation.

The paper develops high-dimensional spectral flow cytometry panels for immune phenotyping in healthy donors and applies them to study immune variation in autoimmune diseases.