C. Chang

Modulation of neurofluid fluctuation frequency by baseline carbon dioxide in awake humans: the role of the autonomic nervous system

This study investigates how baseline CO2 levels modulate neurofluid dynamics in awake humans, showing that autonomic nervous system regulation affects flow through frequency changes rather than amplitude.

Molecular basis for dual functions in pilus assembly modulated by the lid of a pilus-specific sortase

This study identifies the structural basis for the dual function of SrtC2 in pilus assembly by showing how its extended lid modulates polymerization and anchoring activities.

Genome sequencing of 'Fuji' apple clonal varieties reveals evolutionary history and genetic mechanism of the spur-type morphology

The paper sequenced clonal Fuji apple varieties to uncover somatic variants linked to spur-type morphology and early maturation traits.

Observing one-divalent-metal-ion dependent and histidine-promoted His-Me family I-PpoI nuclease catalysis in crystallo

This study uses time-resolved crystallography to investigate the catalytic mechanism of His-Me family I-PpoI nuclease, revealing the role of a single divalent metal ion and histidine in DNA hydrolysis.

GPX3 supports ovarian cancer tumor progression in vivo and promotes expression of GDF15

This study shows GPX3 is necessary for ovarian cancer growth in vivo and promotes GDF15 expression, linking it to immune checkpoint inhibitor response.

Protein language models-assisted engineering of Uracil-N glycosylase enables programmable T-to-G and T-to-C base editing

This study uses protein language models to engineer a uracil-N-glycosylase variant for programmable T-to-G and T-to-C base editing, achieving high success rates in correcting genetic mutations in mice.

CD4+ T cell senescence is associated with reduced reactogenicity in severe/critical COVID-19

The paper finds that higher levels of CD4+ T cell senescence in severe/critical COVID-19 patients correlate with reduced antibody responses and worse survival, especially in breakthrough infections.

Ubiquitin ligase and signalling hub MYCBP2 is required for efficient EPHB2 tyrosine kinase receptor function

This study identifies MYCBP2 as a critical ubiquitin ligase that stabilizes EPHB2 and facilitates its signaling function through interactions with FBXO45 and ephrin-B ligands.

Mitigation of chromosome loss in clinical CRISPR-Cas9-engineered T cells

This study investigates chromosome loss in CRISPR-Cas9-engineered T cells and shows a modified manufacturing process reduces it while preserving editing efficacy.

The Basal and Major Pilins in the Corynebacterium diphtheriae SpaA Pilus Adopt Similar Structures that Competitively React with the Pilin Polymerase

The paper shows that SpaB and N-terminal SpaA (NSpaA) from Corynebacterium diphtheriae adopt similar structures and compete for a shared reaction intermediate in pilus assembly.

Broad-spectrum antiviral inhibitors targeting pandemic potential RNA viruses

This study identifies broad-spectrum antiviral agents effective against multiple RNA virus families, including arboviruses and respiratory pathogens.

Stromal resistance to castration-induced prostate regression in a mouse model of benign prostatic hyperplasia

This study shows that stromal compartments in a mouse model of BPH are resistant to castration-induced regression, suggesting the need for additional therapies.

Targeting TR4-induced RCC cells-derived exosomally initiated signaling increases Sunitinib efficacy

This study shows TR4 promotes RCC cell-derived exosomal ADAM15, which activates EGFR in endothelial cells and reduces Sunitinib efficacy, and demonstrates that targeting this pathway enhances Sunitinib effectiveness.

Kinetic and Structural Analysis of Ribonucleotide Extension by DNA Polymerase η

This study investigates how DNA polymerase η extends ribonucleotide-terminated primers, showing reduced fidelity and structural changes during extension.

Spatial compartmentalization of signalling imparts source-specific functions on secreted factors

The paper identifies distinct functions of VEGFA from different cell types in skeletal muscle regeneration through time-resolved transcriptomic analysis and functional experiments.

Intratumoral mregDC and CXCL13 T helper niches enable local differentiation of CD8 T cells following PD-1 blockade

The paper identifies intratumoral niches involving mregDC and CXCL13+ Th cells that support CD8 T cell differentiation following PD-1 blockade in HCC patients.

COP I and II dependent trafficking controls ER-associated degradation in mammalian cells

The paper investigates COPI and COPII-dependent trafficking in ERAD by tracking proteins in the ERQC and shows differential effects of inhibiting these pathways on retrotranslocation and ERAD.

Realtime morphological characterization and sorting of unlabeled viable cells using deep learning

COSMOS enables real-time, unlabeled cell sorting based on morphology using deep learning, achieving high enrichment of viable cells for functional analysis.

Shift of lung macrophage composition is associated with COVID-19 disease severity and recovery

This study identifies shifts in lung macrophage composition linked to COVID-19 severity and recovery, suggesting therapeutic strategies targeting macrophage function.

Robustness of DNA Looping Across Multiple Divisions in Individual Bacteria

The paper investigates the robustness of DNA looping in E. coli during cell division, finding that repression persists across multiple generations and is insensitive to repressor concentration and division events.

Soil microbes mediate the effects of environmental variability on plant invasion

The paper shows that environmental variability affects plant invasion indirectly through changes in soil microbial communities, particularly fungal pathogen diversity.

Islet transplantation tolerance in animals with defined histocompatibility and diabetes

This paper develops a mouse model for studying islet transplantation tolerance by creating a congenic line that allows controlled diabetes induction and assessment of islet engraftment.

LRP-1 Commands Two Parallel Signalling Mechanisms to Support Constitutive Tumour Cell Motility in the Absence of Blood Supply

This study shows LRP-1 supports tumour cell motility under ischemic conditions by coordinating two distinct signalling pathways involving Hsp90 and EGFR.

Minimal-invasive enhancement of auditory perception by terahertz wave modulation

This study introduces terahertz wave modulation to enhance hearing perception in animals by increasing cochlear hair cell currents and sensitivity without genetic modification.

Structural Basis for Membrane Recruitment of ATG16L1 by WIPI2 in Autophagy

This study determines the crystal structure of WIPI2 interacting with ATG16L1, revealing the molecular mechanism of ATG16L1 recruitment to the phagophore and its role in autophagy initiation.

A programmable system to methylate and demethylate m6A on specific mRNAs

This paper introduces a CRISPR/dCas13a-based system to methylate and demethylate m6A on specific mRNAs, enabling targeted exploration of m6A's role in biological processes.

Proteins with prion-like domains can form viscoelastic condensates that enable membrane remodeling and endocytosis

The paper shows that prion-like domain-containing endocytic coat proteins form viscoelastic condensates that enable actin-independent membrane remodeling and endocytosis in yeast.

Reconstitution of cargo-induced LC3 lipidation in mammalian selective autophagy

This study investigates how different cargo receptors stimulate LC3 lipidation in selective autophagy using GUVs and shows varying requirements for autophagy core complexes.

An RNAi screen in C. elegans for genes that play a role in secretion and cleavage of VAPB MSP domain.

Magnetotactic bacteria accumulate a large pool of iron distinct from their magnetite crystals

This study quantifies iron pools in Magnetospirillum magneticum AMB-1, showing that magnetite contains at most 30% of total intracellular iron, with a large pool of unmineralized iron detected in the cytoplasm and magnetosomes.