F. Sobott

Swinging lever mechanism of myosin directly demonstrated by time-resolved cryoEM

This paper uses time-resolved cryoEM to directly observe the swinging lever mechanism of myosin-5, showing its structural changes during ATP hydrolysis and force generation.

Rapid Prediction of Lipid Interaction Sites on Pleckstrin Homology Domains Using Deep Graph Neural Networks and Molecular Dynamics Simulations

This study uses deep graph neural networks trained on MD simulations to predict phosphoinositide interaction sites on PH domains, achieving results comparable to simulations in seconds.

DNA binding and bridging by human CtIP in the healthy and diseased states

This study investigates the DNA binding and bridging activities of CtIP in healthy and diseased states, identifying a C-terminal DNA-binding domain and showing that tetramerization is required for DNA bridging.

The folding-limited nucleation of curli hints at an evolved safety mechanism for functional amyloid production

This study investigates the molecular mechanism of curli amyloid formation in Salmonella and Escherichia coli, revealing a folding-limited nucleation process that balances efficient polymerization with avoiding premature aggregation.

A targetable N-terminal motif orchestrates α-synuclein oligomer to fibril conversion

This study identifies an N-terminal motif in α-synuclein oligomers that drives fibril conversion and shows how a mutation disrupts this process, offering a new therapeutic target.

Conformational restriction shapes inhibition of a multidrug efflux adaptor protein

The paper investigates how an inhibitor restricts the conformational dynamics of AcrA, a multidrug efflux adaptor protein, to block drug signal transmission in E. coli.

Systematic simulation of the interactions of Pleckstrin homology domains with membranes

The paper systematically simulates interactions of 100 mammalian PH domains with PIP-containing membranes, revealing conserved and novel interaction sites and mechanisms.

Large-scale conformational changes of FhaC provide insights into the two-partner secretion mechanism

The paper investigates conformational changes in FhaC, a TpsB transporter, revealing how it facilitates protein secretion without external energy sources through intrinsic dynamics.

The bacterial magnesium transporter MgtA reveals highly selective interaction with specific cardiolipin species

This study shows that the bacterial magnesium transporter MgtA has functional specificity for cardiolipin species, with maximum activity achieved when combining cardiolipin 16:0 and 18:1.

Tension mediated mechanical activation and pocket delipidation lead to an analogous MscL state

This study investigates how tension and pocket delipidation affect MscL channel states, finding they lead to similar expanded states but through different pathways.

Vibrio cholerae ParE2 toxin modulates its operon transcription by stabilization of an antitoxin DNA ruler

This study investigates how ParE2 toxin modulates its operon transcription by stabilizing the antitoxin DNA ruler through protein-DNA interactions and structural analysis.

Analysis of the PcrA-RNA polymerase complex reveals a helicase interaction motif and a role for PcrA/UvrD helicase in the suppression of R-loops.

This study identifies a helicase interaction motif in the PcrA-RNAP complex and shows PcrA's role in suppressing R-loops by resolving transcription-replication conflicts.

Entropic pressure controls oligomerization of Vibrio cholerae ParD2 antitoxin

This study shows that the intrinsically disordered C-terminal domain of VcParD2 restricts its oligomerization to stable 10-mer or 12-mer structures due to entropic pressure.

The role of water mobility in protein misfolding

This study shows that water mobility, influenced by ions like NaCl and CsI, affects the aggregation rate of α-synuclein, a protein linked to Parkinson's disease.

Structure of the endocytic adaptor complex reveals the basis for efficient membrane anchoring during clathrin-mediated endocytosis

The paper presents a cryoEM structure of a 16-mer complex from Sla2 and Ent1, showing how they anchor to the plasma membrane during clathrin-mediated endocytosis and highlights the importance of their interactions for efficient membrane invagination.

Discriminative SKP2 interactions with CDK-cyclin complexes support a cyclin A-specific role in p27KIP1 degradation

The paper identifies a cyclin A-specific interaction between SKP2 and CDK2-cyclin A complexes that promotes p27KIP1 degradation, distinguishing cyclin A from other G1 cyclins.

Brazilin Removes Toxic alpha-Synuclein and Seeding Competent Assemblies from Parkinson Brain by Altering Conformational Equilibrium

This paper shows Brazilin inhibits alpha-synuclein fibril formation and reduces toxicity in Parkinson's disease by altering conformational equilibrium.

Need for speed: Examining protein behaviour during cryoEM grid preparation at different timescales

This study examines how different macromolecular complexes behave during cryoEM grid preparation at various timescales using three vitrification devices.

A Cryo-EM Grid Preparation Device for Time-Resolved Structural Studies

This paper introduces a cryo-EM grid preparation device that enables rapid mixing and vitrification, achieving sub 4 Å reconstructions for time-resolved structural studies.

The C-Terminal Domain Of ParB Is Critical For Dynamic DNA Binding And Bridging Interactions Which Condense The Bacterial Centromere

This study identifies the C-terminal domain of ParB as critical for DNA binding and bridging interactions that drive bacterial chromosome condensation.