J. Sharp

Long-term maintenance of patient-specific characteristics in tumoroids from six cancer indications in a common base culture media system

This study shows that tumoroids from six cancer types can be maintained long-term in a standardized serum-free medium, preserving their genetic and phenotypic features.

Tuning single-molecule ClyA nanopore tweezers for real-time tracking ofthe conformational dynamics of West Nile viral NS2B/NS3 protease

This study develops a nanopore tweezers platform to track conformational dynamics of West Nile viral NS2B/NS3 protease in real-time and reveals its conformational energy landscape.

Hyaluronidase impacts exposures of long-acting injectable paliperidone palmitate in rodent models

This study evaluates how hyaluronidase affects the release and pharmacokinetics of a long-acting injectable drug in mice and rats.

The effect of molnupiravir and nirmatrelvir on SARS-CoV-2 genome diversity in severe models of COVID-19.

This study examines how molnupiravir and nirmatrelvir affect SARS-CoV-2 genome diversity in immunocompromised mice, finding reduced viral load and specific mutations but no major consensus sequence changes.

Differential nuclear import determines lncRNA inheritance following mitosis

The paper investigates RNA inheritance after mitosis, finding that half of nuclear RNAs are inherited transcription-independently, with snRNAs like U2 being retained while lncRNAs like NEAT1 and MALAT1 are not.

Validity Assessment of Michigan's Proposed qPCR Threshold Value for Rapid Water-Quality Monitoring of E. coli Contamination

The paper assesses the validity of Michigan's proposed qPCR threshold value for E. coli by comparing its performance with culture-based methods on test and training data.

Lack of antiviral activity of probenecid in Vero E6 cells and Syrian golden hamsters: a need for better understanding of inter-lab differences in preclinical assays.

This paper investigates probenecid's antiviral activity against SARS-CoV-2 in Vero E6 cells and Syrian golden hamsters, finding no significant inhibition of viral replication or cytopathic effects.

Ronapreve (REGN-CoV; casirivimab and imdevimab) reduces the viral burden and alters the pulmonary response to the SARS-CoV 2 Delta variant (B.1.617.2) in K18-hACE2 mice using an experimental design reflective of a treatment use case

This study evaluates Ronapreve's efficacy against SARS-CoV-2 Delta and Omicron variants in mice, showing reduced viral burden and altered pulmonary response for Delta but not Omicron.

Evaluation of intranasal nafamostat or camostat for SARS-CoV-2 chemoprophylaxis in Syrian golden hamsters

This study evaluates intranasal nafamostat and camostat for preventing SARS-CoV-2 infection in Syrian golden hamsters, finding nafamostat effective in keeping viral RNA undetectable for 7 days.

Quantitation of tizoxanide in multiple matrices to support cell culture, animal and human research.

The paper describes an LC-MS/MS assay validated for quantifying tizoxanide in human plasma, mouse plasma, and DMEM with FBS, showing high accuracy and precision.

The degree of polymerization and sulfation patterns in heparan sulfate are critical determinants of cytomegalovirus entry into host cells

Naturally-acquired immunity in Syrian Golden Hamsters provides protection from re-exposure to emerging heterosubtypic SARS-CoV-2 variants B.1.1.7 and B.1.351

This study shows that prior SARS-CoV-2 infection in Syrian Golden Hamsters protects against re-exposure to variants B.1.1.7 and B.1.351, suggesting immunity may limit disease from emerging variants.

Development of a highly sensitive bioanalytical assay for the quantification of favipiravir.

This paper develops a sensitive LC-MS/MS assay for quantifying favipiravir, showing high accuracy and precision across a wide concentration range.

Optimisation and validation of a sensitive bioanalytical method for niclosamide.

The paper develops and validates a sensitive LC-MS/MS assay for niclosamide to study its pharmacokinetics and potential use in preclinical and clinical evaluations.

Sequential infection with influenza A virus followed by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) leads to more severe disease and encephalitis in a mouse model of COVID-19.

This study shows that sequential infection with influenza A virus followed by SARS-CoV-2 in mice leads to more severe disease and encephalitis compared to single infections.

Allosteric regulation of lysosomal enzyme recognition by the cation-independent mannose 6-phosphate receptor

This study investigates how the CI-MPR receptor binds and releases carbohydrates through allosteric domain cooperativity using biophysical methods and crystal structures.