L. Turtle

Deep proteomics network and machine learning analysis of human cerebrospinal fluid in Japanese encephalitis virus infection

This study identifies a ten-protein diagnostic signature for Japanese encephalitis virus infection in cerebrospinal fluid with >99% accuracy using proteomics and machine learning.

Rapid selection of P323L in the SARS-CoV-2 polymerase (NSP12) in humans and non-human primate models and confers a large plaque phenotype

The paper tracks the rapid emergence of the P323L mutation in SARS-CoV-2 polymerase in human and non-human primate models, showing it confers a larger plaque phenotype compared to P323.

The impact of viral mutations on recognition by SARS-CoV-2 specific T-cells

The paper identifies SARS-CoV-2 T-cell epitope variants that reduce recognition by T-cells, highlighting the risk of immune evasion.

Sequence analysis of SARS-CoV-2 in nasopharyngeal samples from patients with COVID-19 illustrates population variation and diverse phenotypes, placing the in vitro growth properties of B.1.1.7 and B.1.351 lineage viruses in context.

The paper analyzes SARS-CoV-2 sequence variation in patient samples and compares the in vitro growth of B.1.1.7 and B.1.351 variants to other isolates, finding no significant difference in growth for B.1.1.7.

Identification and quantification of SARS-CoV-2 leader subgenomic mRNA gene junctions in nasopharyngeal samples shows phasic transcription in animal models of COVID-19 and dysregulation at later time points that can also be identified in humans

The paper identifies and quantifies SARS-CoV-2 leader-TRS-junctions in samples, showing phasic sgmRNA transcription in animal models and dysregulation in humans.

Molecular mechanisms underlying attenuation of live attenuated Japanese encephalitis virus vaccine SA14-14-2

This paper identifies molecular mechanisms of attenuation in the SA14-14-2 Japanese encephalitis vaccine strain, showing loss of NS1 and envelope protein folding defects lead to enhanced immune responses.

A systematic evaluation of IgM and IgG antibody assay accuracy in diagnosing acute Zika Virus infection in Brazil; lessons relevant to emerging infections

This study evaluates the accuracy of IgM and IgG antibody assays for diagnosing acute Zika Virus infection in Brazil, finding that IgG assays outperformed IgM ones and highlighting the need for careful evaluation in populations with co-circulating viruses.

Sequential infection with influenza A virus followed by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) leads to more severe disease and encephalitis in a mouse model of COVID-19.

This study shows that sequential infection with influenza A virus followed by SARS-CoV-2 in mice leads to more severe disease and encephalitis compared to single infections.

Cross-Protection Against Zika Virus Infection Conferred by a Live Attenuated Japanese Encephalitis SA14-14-2 Vaccine

This study shows that the JE vaccine SA14-14-2 protects mice against ZIKV through cross-reactive T cell immunity rather than antibodies.

Broad and strong memory CD4+ and CD8+ T cells induced by SARS-CoV-2 in UK convalescent COVID-19 patients.

This study examines T cell immunity in UK COVID-19 patients, finding stronger and broader memory T cell responses in severe cases, with implications for vaccine design.

Methods of inactivation of SARS-CoV-2 for downstream biological assays

This study tests methods to inactivate SARS-CoV-2 for use in lower containment laboratories, finding that heat, detergents, and UV light effectively inactivate the virus.