K. Chiem

Antivirals against monkeypox infections

This study evaluates 19 compounds for antiviral activity against Orthopoxviruses, identifying 13 with activity against vaccinia virus and 11 with activity against monkeypox virus.

Monitoring SARS-CoV-2 infection using a double reporter-expressing virus

This paper introduces a dual-reporter SARS-CoV-2 virus that expresses both mCherry and Nluc for improved in vitro and in vivo infection monitoring.

Immunization with recombinant accessory protein-deficient SARS-CoV-2 protects against lethal challenge and viral transmission

This study develops a live-attenuated SARS-CoV-2 vaccine by deleting two accessory proteins, showing it protects against viral challenge and transmission in animal models.

Vaccinia virus attenuation by codon deoptimization of the A24R gene for vaccine development

This study attenuates vaccinia virus by codon deoptimization of the A24R gene, creating a safe and protective vaccine candidate against poxvirus infection.

A single amino acid substitution in PB1 of pandemic H1N1 with A/Ann Arbor/6/60 master donor virus mutations as a novel live-attenuated influenza virus vaccine

This paper shows that a single amino acid change in the PB1 protein of the pandemic H1N1 virus can create a live-attenuated vaccine with reduced pathogenicity and transmission.

Bi-reporter vaccinia virus for tracking viral infections in vitro and in vivo

This paper introduces bi-reporter vaccinia viruses expressing both fluorescent and luciferase genes to track viral infections in vitro and in vivo, enabling detailed study of infection dynamics and host-pathogen interactions.

A SARS-CoV-2 nucleocapsid protein TR-FRET assay amenable to high-throughput screening

The paper develops a TR-FRET assay for SARS-CoV-2 NP detection, enabling high-throughput screening of compounds against SARS-CoV-2 and its variants.

A bifluorescent-based assay for the identification of neutralizing antibodies against SARS-CoV-2 variants of concern in vitro and in vivo

This paper develops a bifluorescent-based assay to identify neutralizing antibodies against SARS-CoV-2 variants in vitro and in vivo, showing it accurately reflects viral neutralization.

Visualization of SARS-CoV-2 infection dynamic

This study develops a method to visualize SARS-CoV-2 infection dynamics using reporter-expressing recombinant viruses, enabling real-time tracking and antibody screening in vivo.

Amino acid residues involved in inhibition of host gene expression by influenza A/Brevig Mission/1/1918 PA-X

The paper identifies amino acid residues critical for PA-X's host gene expression inhibition using a bacteria-based system.

Contribution of SARS-CoV-2 accessory proteins to viral pathogenicity in K18 hACE2 transgenic mice

This study identifies ORF3a and ORF6 as key contributors to SARS-CoV-2 pathogenesis in mice using a reverse genetics system and K18 hACE2 transgenic mice.

Extracellular Delivery of Functional Mitochondria Rescues the Dysfunction of CD4+ T Cells in Aging.

This study transfers functional mitochondria into aged CD4+ T cells, improving their metabolism, reducing oxidative stress, and enhancing immune function against infections.

Generation and Characterization of recombinant SARS-CoV-2 expressing reporter genes

This paper generates and characterizes recombinant SARS-CoV-2 viruses expressing fluorescent or bioluminescent reporter genes, demonstrating their utility for tracking viral infection and screening therapeutics.

Therapeutic activity of an inhaled potent SARS-CoV-2 neutralizing human monoclonal antibody in hamsters

This study evaluates an inhaled monoclonal antibody against SARS-CoV-2 in hamsters, showing reduced viral burden and lung pathology with low-dose inhalation.

Rapid in vitro assays for screening neutralizing antibodies and antivirals against SARS-CoV-2

This paper describes a rapid and reproducible plaque reduction microneutralization assay for identifying neutralizing antibodies and antivirals against SARS-CoV-2, suitable for high-throughput screening.

Lethality of SARS-CoV-2 infection in K18 human angiotensin converting enzyme 2 transgenic mice

This study demonstrates that K18 hACE2 transgenic mice are susceptible to SARS-CoV-2 infection, exhibit cytokine and chemokine storms, and serve as a model for studying viral pathogenesis and therapeutic development.

Restoration of Susceptibility to Amikacin by 8-Hydroxyquinoline Analogs Complexed to Zinc

This study evaluates 8-hydroxyquinoline analogs complexed with zinc to restore amikacin susceptibility in AAC(6)-Ib-producing Acinetobacter baumannii strains.

Identification of a Small Molecule Inhibitor of the Aminoglycoside 6’-N-Acetyltransferase Type Ib [AAC(6’)-Ib] Using Mixture-Based Combinatorial Libraries

The paper identifies a small molecule inhibitor of AAC(6’)-Ib using combinatorial libraries and evaluates its efficacy against antibiotic-resistant bacteria.