P.-H. Wu

qMAP Enabled Microanatomical Mapping of Human Skin Aging

This study introduces qMAP, a deep learning-based workflow for microanatomical mapping of human skin, identifying 10 core processes strongly associated with aging and achieving age prediction accuracy comparable to epigenetic clocks.

Engineering self-propelled tumor-infiltrating CAR T cells using synthetic velocity receptors

This paper develops velocity receptors (VRs) to enhance CAR T cell migration through solid tumors, improving tumor infiltration and survival.

Three-dimensional assessments are necessary to determine the true, spatially-resolved composition of tissues

This study shows that 3D assessments are necessary to accurately determine tissue composition and neoplastic content, as 2D methods under-sample spatial heterogeneity.

Colonization of Bacillus altitudinis on the Compatible Soybean Varieties to Provide Seed Rot Resistance

This study identifies Bacillus altitudinis as a seed-associated bacterium that provides seed rot resistance against Calonectria ilicicola but only in compatible soybean varieties.

Precision-engineered biomimetics: the human fallopian tube

The paper develops a precision-engineered multi-compartment organoid model of the human fallopian tube validated against a 3D single-cell reference map.

Three-dimensional genomic mapping of human pancreatic tissue reveals striking multifocality and genetic heterogeneity in precancerous lesions

This study uses 3D imaging and genomic analysis to show that human pancreatic precancerous lesions (PanINs) are highly multifocal and genetically heterogeneous.

PanIN and CAF Transitions in Pancreatic Carcinogenesis Revealed with Spatial Data Integration

This paper develops a new protocol for spatial transcriptomics analysis of FFPE PanIN specimens and integrates it with imaging and scRNA-seq to study the progression from PanIN to PDAC.

Mechanotransductive feedback control of endothelial cell motility and vascular morphogenesis

This study identifies a mechanotransductive feedback loop involving YAP and TAZ that regulates endothelial cell motility and vascular morphogenesis in vitro and in vivo.

Quantum CART (qCART), a piggyBac-based system for development and production of virus-free multiplex CAR-T cell therapy

The paper introduces a virus-free multiplex CAR-T cell therapy system called qCART, integrating four platforms to improve CAR-T development and manufacturing.

Manufacturing CD20/CD19-targeted iCasp9 regulatable CAR-TSCM cells using qCART, the Quantum pBac-based CAR-T system

This paper demonstrates the efficient manufacturing of CD20/CD19 dual-targeted, iCasp9-regulatable CAR-TSCM cells using the virus-free qCART system, showing robust tumor eradication and safety.

Deep Learning Identification of Stiffness Markers in Breast Cancer

This study uses deep learning to identify stiffness markers in breast cancer tissue, finding that straight collagen percentage best correlates with tissue stiffness.

In situ characterization of the 3D microanatomy of the pancreas and pancreatic cancer at single cell resolution

The paper develops a method to reconstruct 3D pancreatic tissue at single-cell resolution and maps cancer invasion modes in the tumor microenvironment.

E-cadherin interacts with EGFR resulting in hyper-activation of ERK in multiple models of breast cancer

This study shows E-cadherin interacts with EGFR to hyper-activate ERK, promoting proliferation in breast cancer models and identifying a potential therapeutic target.

Multi-compartment tumor organoids

This paper develops a high-throughput microtechnology to create multi-compartment tumor organoids that mimic tumor microenvironments and improve drug response and tumor progression studies.

Fractional re-distribution among cell motility states during ageing

The paper shows that ageing reduces cell motility heterogeneity by redistributing cells among motility states rather than uniformly decreasing motility.

The role of integrin αv and CD44 in GBM migration in human brain

This study uses human organotypic brain slices to examine GBM cell motility, finding that CD44 knockdown reduces motility more than ITGAV knockdown.