L. Chang

New structural features of the APC/C revealed by high resolution cryo-EM structures of apo-APC/C and the APC/C CDH1:EMI1 complex

This study uses cryo-EM to determine high-resolution structures of apo-APC/C and APC/C CDH1:EMI1, revealing new structural features and regulatory mechanisms.

Size evolution of gigantic genomes suggests stochastic outcomes of transposable element/host silencing interactions

The paper examines TE/host interactions in salamander genomes and finds no correlation between genome size and TE deletion rates or suppression, suggesting stochastic TE accumulation in gigantic genomes.

Chemically induced cell plasticity enables the generation of high-fidelity embryo model

This paper develops a chemical-only method to generate high-fidelity embryo models by inducing cell plasticity in stem cells, resulting in embryo-like structures that recapitulate early developmental stages.

TrkA+ sensory neurons regulate osteosarcoma proliferation and vascularization to promote disease progression

This study shows that TrkA+ sensory neurons promote osteosarcoma progression by enhancing tumor growth, vascularization, and metastasis, and that inhibiting these neurons reduces disease severity and improves survival.

Identification of Aggregation Pheromone in Odontothrips loti (Thysanoptera: Thripidae)

This study identifies (R)-lavandulyl (R)-2-methylbutanoate as the aggregation pheromone of Odontothrips loti, showing attraction in behavioral and electrophysiological assays.

The phosphoproteome is a first responder in tiered cellular adaptation to chemical stress followed by proteomics and transcriptomics alteration

This study compares transcriptomics, proteomics, and phosphoproteomics responses in HepG2 and AC16 cells to chemical exposure, finding phosphoproteomics changes occur earlier and at lower concentrations than transcriptomics.

Cooperativity and induced oligomerisation control the interaction of SARS- CoV-2 with its cellular receptor and patient-derived antibodies

The paper investigates how multivalent interactions between SARS-CoV-2 spike protein, ACE2 receptor, and patient-derived antibodies influence viral entry and antibody inhibition.

Single cell RNA analysis uncovers the cell differentiation trajectories and functionalization for air breathing of frog lung

This study uses single-cell RNA sequencing to identify cell types and differentiation trajectories in frog lungs, revealing functionalization for air breathing and evolutionary insights.

Engineering of a biosensor for intracellular aspartate

The paper develops a biosensor, iAspSnFR, to measure intracellular aspartate levels in live cells and demonstrates its utility in studying metabolic pathways and cancer biology.

SICKO: Systematic Imaging of Caenorhabditis Killing Organisms

SICKO enables longitudinal tracking of microbial colonization and host survival in C. elegans, revealing that gut colonization by E. coli or Pseudomonas aeruginosa shortens lifespan and that pmk-1-deficient animals are more susceptible.

Microsecond pulse electrical stimulation modulates cell migration

This study introduces microsecond pulse electric field (sPEF) to enhance wound healing by promoting fibroblast migration and ECM alignment, validated in 2D and 3D models and a mouse wound healing assay.

Spatial compartmentalization of signalling imparts source-specific functions on secreted factors

The paper identifies distinct functions of VEGFA from different cell types in skeletal muscle regeneration through time-resolved transcriptomic analysis and functional experiments.

Call patterns encode and transmit emotion in marmoset monkeys

The paper analyzes marmoset monkey vocalizations to show that call patterns encode emotional states and transmit emotion between individuals.

DeepTracer ID: De Novo Protein Identification from Cryo-EM Maps

DeepTracer-ID identifies proteins from cryo-EM maps without additional sequence information by combining DeepTracer modeling with AlphaFold2 predictions.

Structural characterization of a breast cancer-associated mutation in caveolin-1

This study examines how a breast cancer-associated mutation in CAV1, P132L, affects its structure and oligomerization, revealing its impact on caveolae formation.

Using NAMs to characterize chemical bioactivity at the transcriptomic, proteomic and phosphoproteomic levels

This paper integrates multi-omic data to characterize chemical bioactivity at transcriptomic, proteomic, and phosphoproteomic levels, showing how early phosphoproteomic changes drive downstream effects.

A consensus view on the folding mechanism of protein G, L and their mutants

The paper uses simulations and Bayesian inference to analyze the folding mechanisms of protein G, L, and their mutants, identifying distinct pathways and validating results with experimental data.

Single cell Transcriptome and T cell Repertoire Mapping of the Mechanistic Signatures and T cell Trajectories Contributing to Vascular and Dermal Manifestations of Behcet Disease

This study maps single-cell transcriptomes and T cell repertoires in Behcet's disease to identify immune mechanisms and T cell trajectories in vascular and dermal manifestations.

AlphaFold encodes the principles to identify high affinity peptide binders

This study applies AlphaFold to identify high-affinity peptide binders by predicting their bound and unbound states in competitive binding scenarios.

NELL-1 regulates the matrisome to alter osteosarcoma disease progression

This study shows NELL-1 promotes osteosarcoma progression by altering the matrisome, with loss of NELL-1 reducing invasion and improving survival in both human and mouse models.

Emergence of an adaptive epigenetic cell state in human bladder urothelial carcinoma evolution

This study identifies a TM4SF1-positive cancer subpopulation in bladder cancer that drives intratumor heterogeneity and is associated with poor prognosis.

Improving the analysis of biological ensembles through extended similarity measures

The paper introduces extended similarity measures to improve structural ensemble analysis of macromolecules, achieving linear computational complexity and better clustering performance.

Soil fauna may buffer the negative effects of drought on alien plant invasion

The paper investigates how drought indirectly affects alien plant invasion through soil fauna, finding that soil fauna reduce invasion by boosting native plants, but this effect weakens under drought.

Cryo-EM reveals new species-specific proteins and symmetry elements in the Legionella pneumophila Dot/Icm T4SS

The paper uses cryo-EM to determine the structure of the Legionella pneumophila Dot/Icm T4SS, identifying new proteins and symmetry elements that contribute to its function.

Insights into early stages of the establishment of host:algal endosymbioses: Genetic responses to live versus heat-killed algae and bacterial prey in a sponge host

This study examines gene expression changes in a sponge host exposed to live and heat-killed algae and bacteria, identifying unique genetic responses to algal symbionts.

Liana optical traits increase tropical forest albedo and reduce ecosystem productivity

This study shows lianas increase forest albedo and reduce productivity through specific optical traits, using radiative transfer models and meta-analysis of optical spectra.

Structural basis for the dimerization-dependent CRISPR-Cas12f nuclease

This study determines the cryo-EM structures of Cas12f to elucidate its dimerization-dependent mechanism for DNA cleavage and substrate recognition.

Gluconeogenic Enzyme PCK1 Deficiency Is Critical for CHK2 O-GlcNAcylation and Hepatocellular Carcinoma Growth upon Glucose Deprivation

This study shows that PCK1 deficiency increases O-GlcNAcylation and promotes HCC growth under glucose deprivation by enhancing CHK2 activity.

Mass-spectrometry-based near complete draft of the Saccharomyces cerevisiae proteome

The paper presents a near-complete yeast proteome dataset using optimized sample preparation and high-resolution mass spectrometry, identifying 5610 proteins with 94.1% coverage of yeast ORFs.

Structure-Based Design with Tag-Based Purification and In-Process Biotinylation Enable Streamlined Development of SARS-CoV-2 Spike Molecular Probes

The paper develops biotin-labeled SARS-CoV-2 spike molecular probes using structure-based design and purification methods, achieving high yields and enabling antibody characterization.