flow cytometry

The paper shows that anti-CTLA-4 ADC treatment causes B cell depletion via T cell hyper-proliferation and regulatory T cell impairment in CTLA-4 knock-in mice.

The paper identifies liver-specific inflammatory signatures that precede clinically significant liver damage in CHB patients after NA withdrawal.

This paper develops a T cell engager that uses IFNγ to upregulate CD38 on leukemia stem cells, enabling their targeting and depletion in AML.

The paper tests tyrosine kinase inhibitors on B cells, finding some drugs target LTK-positive plasma cells and inhibit B cell proliferation.

This study shows that reducing STAT4 in neutrophils lowers atherosclerosis and improves plaque stability by decreasing neutrophil activation and their migration to aortas.

This study shows FAP-CAR-T cells effectively kill glioblastoma cells through bystander mechanisms, even when only some cells express FAP, and demonstrate therapeutic efficacy in vivo.

The paper uses mass cytometry to analyze immune cell subsets in bronchoalveolar lavage fluid from patients with PCP, DI-ILD, and ICI-ILD, revealing distinct immunophenotypes associated with each condition.

This study shows that targeting the α4β7/MAdCAM-1 axis reduces proinflammatory T cell recruitment to the liver, thereby suppressing liver fibrosis progression in a mouse model.

This study shows that deleting Trib1 shifts exhausted T cells to a KLR+ CD8+ effector state, enhancing viral clearance and checkpoint inhibitor response.

This study identifies T cell dysregulation, inflammation, and mis-coordinated adaptive immune responses in Long COVID patients compared to non-LC individuals.

This study demonstrates that cryopreservation of lymphoid tissue allows for the study of non-hematopoietic stromal cells in human disease, preserving cell type proportions and transcriptional profiles.

This study shows that CpG-ODN improves survival in a mouse LAM model by enhancing immune responses, mediated by plasmacytoid dendritic cells, and synergizes with anti-PD1 and rapamycin.

The paper examines immune responses in vaccinated individuals during SARS-CoV-2 breakthrough infection, showing early activation of memory T cells and subsequent neutralizing antibody production.

This study shows that Lantana camara leaf extract inhibits MDA-MB-231 breast cancer cell survival and migration through apoptosis and cell cycle arrest.

This study shows naive T lymphocytes chemotax to CCL21 but not to S1P-rich serum, using in vitro assays to quantify migration responses.

The paper identifies CD39+ CD4+ T cells in tumors with exhaustion and cytotoxic traits, showing their expansion with CTLA-4 blockade and association with better survival in breast cancer.

This study identifies immune cell dysregulation in hidradenitis suppurativa using mass cytometry and RNA-seq, finding increased CD38 expression in monocytes and suggesting CD38 as a therapeutic target.

The paper introduces a ghost cytometry-based method for fast pooled CRISPR screening of high-content cellular phenotypes, including fluorescence and label-free modes, enabling genome-wide analysis of gene effects on cellular processes.

The paper examines immune changes at the fetal-maternal interface in GDM, obesity, and healthy pregnancies, finding dysregulated PD-1/PD-L1 axis and reduced Treg infiltration in GDM.

The paper describes the generation of a transformed murine mast cell line, PMC-306, and identifies the loss of Cdkn2a/Arf as the cause of its immortalization.

This study shows that TLR3 agonist hiltonol sequesters TAZ in NK cells, enhancing cytotoxicity by increasing contractility and reducing inhibitory receptors.

This study uses a murine model to show that C57Bl/6 mice can control multiple myeloma through IFN-gamma-dependent immune responses, highlighting the model's utility for studying anti-myeloma immunity.

The paper introduces RepairSwitch, a flow cytometry assay to simultaneously measure HR and NHEJ DNA repair rates in living cells, and demonstrates its utility in assessing drug and genetic perturbations.

The paper links early brain beta-amyloid accumulation with peripheral T cell changes using mass cytometry and machine learning in AD cohorts.

This study investigates the dynamics of innate immune response in pulpitis by analyzing immune cell composition and bacterial infiltration in mouse dental pulp over time.

This study develops an in vitro cerebrovascular model and validates computational fluid dynamics simulations of flow and embolus migration in the brain.

The paper uses mass cytometry to identify distinct immune cell subsets in BALF from ILD patients, revealing disease-specific immune signatures.

This study identifies changes in T-cell subsets in bovine blood and milk during subclinical Staphylococcus aureus mastitis, showing activation of CD4+, CD8+, and γδ T-cells and differences in naive and memory T-cell proportions.