K. Aoki

ErbB2/HER2 governs CDK4 inhibitor sensitivity and timing and irreversibility of G1/S transition by altering c-Myc and cyclin D function

This study shows how ErbB2 levels affect CDK4 inhibitor sensitivity and the timing of G1/S transition by altering c-Myc and cyclin D function in breast cancer cells.

Rapid and reversible dissolution of biomolecular condensates using light-controlled recruitment of a solubility tag

This paper introduces OptoMBP, a light-controlled method to rapidly and reversibly dissolve biomolecular condensates, demonstrating its application in reversing FUS-CHOP-driven transcriptional changes.

Live-cell fluorescence imaging and optogenetic control of PKA kinase activity in fission yeast Schizosaccharomyces pombe.

This paper develops a biosensor to visualize and manipulate PKA activity in fission yeast, revealing its dynamics during cell cycle phases.

ORP9-PH domain-based fluorescent reporters for visualizing phosphatidylinositol 4-phosphate dynamics in living cells

The paper develops fluorescent PI4P reporters based on ORP9-PH domains to visualize PI4P dynamics in living cells across multiple locations with high specificity.

Cytoplasmic fluidization triggers breaking spore dormancy in fission yeast.

This study identifies a signaling pathway in fission yeast spores that fluidizes the cytoplasm during dormancy breaking, enabling rapid cellular responses to glucose.

Uncovering novel endolysins against methicillin-resistant Staphylococcus aureus using microbial single-cell genome sequencing

The paper uses microbial single-cell genome sequencing to discover novel endolysins against methicillin-resistant Staphylococcus aureus, identifying a lead endolysin with effective antimicrobial properties.

Live-cell imaging provides direct evidence for a threshold in CDK activity at the G2/M transition

The paper uses live-cell imaging to show CDK activity reaches a threshold at G2/M transition in fission yeast and mammalian cells.

Novel monoclonal antibodies showing broad neutralizing activity for SARS-CoV-2 variants including Omicrons BA.5 and BA.2.75

Comprehensive analysis of platelet glycoprotein Ibα glycosylation.

This study identifies O- and N-glycosites on GPIb and characterizes its glycan structures using mass spectrometry.

Optimal inference of molecular interactions in live FRET imaging

This paper introduces B-FRET, a Bayesian framework for inferring molecular interactions from live FRET imaging data, improving signal-to-noise ratio without additional measurements.

Hyperconcentrated Mucus Unifies Submucosal Gland and Superficial Airway Dysfunction in Cystic Fibrosis

This study identifies hyperconcentrated mucus as a unifying mechanism for submucosal gland and superficial airway dysfunction in cystic fibrosis.

Quantitative live-cell fluorescence imaging of GPCR downstream signaling dynamics at the single-cell level.

The paper uses live-cell fluorescence imaging to study GPCR downstream signaling dynamics, revealing heterogeneity among receptors and cells.

In vivo study on the healing of bone defect treated with non-thermal atmospheric pressure gas discharge plasma

This study investigates the effect of non-thermal atmospheric pressure gas discharge plasma on bone regeneration in rabbits, finding that 10 minutes of treatment significantly increased new bone volume compared to controls.

Desiccation-induced fibrous condensation of CAHS protein from an anhydrobiotic tardigrade

This study investigates the self-assembling properties of CAHS1 protein from tardigrades under desiccation conditions, showing it forms hydrogels and condensates that may contribute to desiccation tolerance.

Redundant and specific roles of EGFR ligands in the ERK activation waves during collective cell migration of MDCK cells

The paper investigates the roles of EGFR ligands in ERK activation waves during MDCK cell migration, finding redundancy and specific contributions among the ligands.

Near-infrared imaging in fission yeast by genetically encoded biosynthesis of phycocyanobilin

This paper demonstrates that phycocyanobilin enhances iRFP fluorescence in fission yeast, enabling live-cell near-infrared imaging through engineered biosynthesis systems.

Optogenetic relaxation of actomyosin contractility uncovers mechanistic roles of cortical tension during cytokinesis

The paper introduces OptoMYPT, an optogenetic tool to relax actomyosin contractility, revealing cortical tension's role in cytokinesis by accelerating furrow ingression.

Hierarchical modeling of mechano-chemical dynamics of epithelial sheets across cells and tissue

The paper develops a hierarchical mathematical framework to model mechano-chemical dynamics in epithelial sheets, linking single-cell behavior to tissue-level collective migration through ERK activation waves.

Experimental evaluation of thermodynamic speed limit in living cells via information geometry

The paper experimentally tests the thermodynamic speed limit in living cells using fluorescence imaging data to quantify the intrinsic speed of cell proliferation.

A self-exciting point process to study multi-cellular spatial signaling patterns

This paper introduces a spatiotemporal model of dynamic cell signaling using Hawkes processes to capture both single-cell and spatial signaling behaviors in multi-cellular systems.

Virus-Receptor Interactions of Glycosylated SARS-CoV-2 Spike and Human ACE2 Receptor

The paper characterizes glycosylation of SARS-CoV-2 Spike and ACE2 using glycomics and simulations to understand their interactions and guide immunogen design.

Development of optogenetic tools to manipulate cell cycle checkpoints

This study develops optogenetic tools to control cell-cycle checkpoints using phytochrome B in fission yeast, enabling light-induced arrest and release of G2/M and SAC checkpoints.

Oncogenic mutation or overexpression of oncogenic KRAS or BRAF is not sufficient to confer oncogene addiction.

This study shows that oncogenic KRAS or BRAF mutations or overexpression alone are insufficient to induce oncogene addiction in MCF-10A cells.

A genetically encoded red fluorescence dopamine biosensor enables dual imaging of dopamine and norepinephrine

This paper develops a red fluorescent dopamine biosensor with high selectivity over norepinephrine, enabling dual-color imaging of both neuromodulators in vitro.

ERK-mediated mechanochemical waves direct collective cell polarization

This study shows that ERK-mediated mechanochemical waves coordinate intercellular signaling to direct collective cell migration through long-range polarity alignment.

CUB domain-containing protein 1 controls HGF responses by integrating Src and Met-STAT3 signaling

This study identifies CDCP1 as a key regulator of HGF signaling by integrating Src and Met-STAT3 pathways, showing its role in cell invasion and renal growth.

Single-cell quantification of the concentration and dissociation constant of endogenous proteins

The paper quantifies endogenous protein concentration and dissociation constant in living cells using CRISPR/Cas9 and FCCS, revealing dynamic changes in protein interactions.

Cell-to-cell heterogeneity in p38-mediated cross-inhibition of JNK causes stochastic cell death

The paper investigates how p38 cross-inhibits JNK, leading to cell-to-cell variability in JNK activity and contributing to stochastic cell death under stress.