S. A. Malaker

Xylosyltransferase Bump-and-hole Engineering to Chemically Manipulate Proteoglycans in Mammalian Cells

This paper develops a chemical genetic strategy to manipulate proteoglycan glycan attachment sites in mammalian cells using modified xylosyltransferases.

Optimized mucin-selective enrichment strategy to probe the mucinome

The paper optimizes a mucin-selective enrichment strategy to improve throughput, reduce sample input, and enhance glycopeptide signal for mucinome analysis.

False positive glycopeptide identification via in-FAIMS fragmentation

This study identifies a phenomenon called 'in FAIMS fragmentation' that increases spectral matches in glycopeptide analysis, highlighting potential benefits and limitations of FAIMS for O-glycoproteomics.

Glycoproteomic landscape and structural dynamics of TIM family immune checkpoints enabled by mucinase SmE

The paper uses a mucinase to analyze glycoproteomic features of TIM family proteins and shows how glycosylation affects their structure through molecular dynamics simulations.

Stress-Induced Mucin 13 Reductions Drive Intestinal Microbiome Shifts and Despair Behaviors

This paper identifies mucin 13 as a stress-induced regulator of microbiome shifts and despair behaviors in mice.

O-Linked Sialoglycans Modulate the Proteolysis of SARS-CoV-2 Spike and Likely Contribute to the Mutational Trajectory in Variants of Concern

This paper shows that O-linked sialoglycans affect SARS-CoV-2 spike proteolysis by furin and TMPRSS2, and may drive mutations in variants of concern.

Mutational screens highlight glycosylation as a modulator of CSF3R activity

The paper identifies glycosylation sites as critical for CSF3R activity and shows that mutations at these sites can lead to ligand-independent cell growth in chronic neutrophilic leukemia.

Comprehensive analysis of platelet glycoprotein Ibα glycosylation.

This study identifies O- and N-glycosites on GPIb and characterizes its glycan structures using mass spectrometry.

Design of a mucin-selective protease for targeted degradation of cancer-associated mucins

This paper designs a mucin-selective protease conjugated to a nanobody to degrade cancer-associated mucins, showing reduced tumor growth in mouse models.

Novel antibodies for the simple and efficient enrichment of native O-GlcNAc modified peptides

The paper introduces new anti-O-GlcNAc antibodies for efficient enrichment of native O-GlcNAc modified peptides, identifying over 1,300 unique peptides with reduced sample and time requirements.

Revealing the human mucinome

This study develops a mucinomics platform using an inactive mucinase to enrich and identify mucin-domain glycoproteins, revealing molecular signatures in cancer samples.

On-tissue spatially-resolved glycoproteomics guided by N-glycan imaging reveal global dysregulation of canine glioma glycoproteomic landscape

This paper develops a spatially-resolved glycoproteomics approach combining MALDI imaging and LC-MS/MS to identify glycan patterns in canine gliomas, revealing dysregulated glycosylation in tumor and necrotic regions.

Canonical and cross-reactive binding of NK cell inhibitory receptors to HLA-C allotypes is dictated by peptides bound to HLA-C

This study shows that HLA-C bound peptides influence KIR binding specificity, with KIR2DL1 showing broader peptide tolerance than KIR2DL2/3, and cross-reactive binding being highly peptide selective.