M. Lakadamyali

Multiplexed DNA-PAINT Imaging of the Heterogeneity of Late Endosome/Lysosome Protein Composition

This study uses multiplexed DNA-PAINT imaging to reveal cell-type specific subpopulations of late endosomes/lysosomes with unique protein compositions.

Piggybacking functionalized DNA nanostructures into live cell nuclei

This study demonstrates the delivery of DNA origami nanorods into live cell nuclei using an antibody against RNA Polymerase II, showing structural integrity and sub-diffusive motion within the nucleus.

ECLiPSE: A Versatile Classification Technique for Structural and Morphological Analysis of Super-Resolution Microscopy Data

ECLiPSE is a machine learning pipeline for classifying super-resolution microscopy data, achieving high accuracy in identifying cellular structures and applying to tau protein clearance and TDP-43 morphotypes.

Active Transcription and Epigenetic Reactions Synergistically RegulateMeso-Scale Genomic Organization

This study introduces a mesoscale model to show how transcription and epigenetic reactions regulate heterochromatin domain sizes, finding that transcription loss increases domain size and compaction.

Detyrosination enrichment on microtubule subsets is established by the interplay between a stochastically-acting enzyme and microtubule stability

The paper investigates how detyrosination of microtubules occurs on specific subsets through stochastic enzyme activity and microtubule stability interactions.

Chemo-Mechanical Cues Modulate Nano-Scale Chromatin Organization in Healthy and Diseased Connective Tissue Cells

This study shows that chemo-mechanical cues influence chromatin organization in connective tissue cells, with disease disrupting this relationship.

Kinesin-4 Motor Teams Effectively Navigate Dendritic Microtubule Arrays Through Track Switching and Regulation of Microtubule Dynamics

This study shows KIF21B kinesin-4 motors navigate mixed polarity microtubules in dendrites through directional switching and microtubule dynamics regulation.

Cega: A Single Particle Segmentation Algorithm to Identify Moving Particles in a Noisy System

The paper introduces Cega, a single particle segmentation algorithm that improves particle tracking in noisy systems by using KL divergence to distinguish moving particles from background noise.

ORP1L regulates dynein clustering on endolysosmal membranes in response to cholesterol levels

This study shows ORP1L regulates dynein clustering on endolysosomal membranes in response to cholesterol levels, affecting endolysosome positioning.

Tau forms oligomeric complexes on microtubules that are distinct from pathological oligomers in disease

The paper uses SMLM to show physiological tau oligomers on microtubules differ from pathological oligomers and links phosphorylation states to aggregate species.

Shear flow-driven actin re-organization induces ICAM-1 nanoclustering on endothelial cells that impact T-cell migration

This study shows that shear forces induce ICAM-1 nanoclustering on endothelial cells through actin re-organization, enhancing T-cell migration by strengthening adhesion and reducing actin retrograde flow.

Membrane receptor MerTK is a newly identified transcriptional regulator that associates to chromatin as nanoclusters during human DC differentiation

This study identifies MerTK as a transcriptional regulator in human dendritic cells, showing its nuclear translocation and clustering during differentiation.

A Pairwise Distance Distribution Correction (DDC) algorithm to eliminate blinking-caused artifacts in super-resolution microscopy

The paper introduces DDC, a method to correct blinking artifacts in SMLM by using true pairwise distance distributions, improving spatial reconstruction accuracy.

H3K9me2 orchestrates inheritance of spatial positioning of peripheral heterochromatin through mitosis

This study shows H3K9me2 maintains spatial positioning of heterochromatin during mitosis, ensuring inheritance of nuclear organization in daughter cells.

Nanoscale decompaction of nucleosomal DNA revealed through multi-color super-resolution microscopy

The paper uses super-resolution microscopy to show that DNA is compacted by nucleosome groups (clutches) and that hyperacetylation reduces clutch DNA compaction, especially in regions with multiple nearby clutches.

Quantifying motor protein copy number in super-resolution using an imaging invariant calibration

This study quantifies motor protein copy number in super-resolution images using a DNA origami-based calibration method, showing dynein exists as single motors in cytosol and multimers on microtubules.