Q. Tang

Modifications to the gut microbiome alter bone matrix proteomics and fracture toughness at the cellular scale

This paper shows that altering the gut microbiome changes bone matrix proteomics and reduces fracture toughness, with micropillar splitting as a precise method to measure these effects.

KMT2d regulates tooth enamel development

This study investigates the role of KMT2D in amelogenesis using a conditional knockout mouse model, revealing its critical role in enamel development and identifying direct target genes involved in ameloblast differentiation.

RSG-YOLO: Detection of rice seed germination rate based on enhanced YOLOv8 and multi-scale attention feature fusion

This paper introduces RSG-YOLO, an enhanced YOLOv8 model for rice seed germination rate detection, achieving a 4% improvement in mAP50 over YOLOv8.

Structural and biochemical characterization of LIG1 during mutagenic nick sealing of oxidatively damaged ends at the final step of DNA repair

This study characterizes LIG1's structural and biochemical interactions with oxidatively damaged DNA during the final steps of base excision repair.

Arabidopsis GENOMES UNCOUPLED PROTEIN1 binds to plastid RNAs and promotes their maturation

This study identifies GUN1's molecular function as RNA binding and maturation by analyzing plastid transcriptomes and validating targets through RNA immunoprecipitation.

The ESCRT protein CHMP5 promotes T cell leukemia by controlling BRD4-p300-dependent transcription

This paper identifies CHMP5 as a nuclear factor that promotes T cell leukemia by enhancing BRD4-p300-dependent transcription at enhancers and super-enhancers.

Structure-Guided Mutagenesis Targeting Interactions between pp150 Tegument Protein and Small Capsid Protein Identify Five Lethal and Two Live Attenuated HCMV Mutants

The paper identifies five lethal and two live attenuated HCMV mutants by mutating pp150-capsid interactions, revealing critical residues for viral replication.

RECON gene disruption enhances host resistance to enable genome-wide evaluation of intracellular pathogen fitness during infection.

This paper uses a RECON-/- mouse model to perform Tn-seq screening of Listeria monocytogenes in vivo, identifying novel genes critical for bacterial survival and revealing the role of the D-allose operon in pathogenesis.

Alteration in Gut Microbiome and Intestinal Barrier Function caused by Efavirenz versus Dolutegravir Treatments in Mice

This study compares the effects of EFV and DTG on gut microbiome and intestinal barrier function in mice, finding both drugs impair gut barrier integrity, with EFV causing more severe metabolic and structural changes.

Uncovering the Vital Role of Lipid Droplets in Coronavirus Replication: A Novel Insight Arising from the Biological Activity of 2-Bromopalmitate

This study shows that 2-Bromopalmitate inhibits coronavirus replication by disrupting lipid droplets, not through palmitoylation inhibition.

Asymmetric conformations and lipid interactions shape the ATP-coupled cycle of a heterodimeric ABC transporter

This study uses cryo-EM, DEER, and MD simulations to analyze the conformational dynamics of BmrCD, revealing asymmetric ATP and substrate interactions that drive its transport cycle.

ECLiPSE: A Versatile Classification Technique for Structural and Morphological Analysis of Super-Resolution Microscopy Data

ECLiPSE is a machine learning pipeline for classifying super-resolution microscopy data, achieving high accuracy in identifying cellular structures and applying to tau protein clearance and TDP-43 morphotypes.

Overexpression of the violaxanthin de-epoxidase confers faster NPQ and photosynthesis induction in rice

This study shows overexpressing VDE in rice speeds up NPQ and photosynthesis induction, reduces photoinhibition, and increases biomass by 11-16% in field conditions.

Structures of LIG1 active site mutants reveal the importance of DNA end rigidity for mismatch discrimination

This study investigates how mutations in LIG1's active site affect its ability to discriminate between correct and mismatched DNA ends, revealing the role of DNA end rigidity in ligation fidelity.

Step-wise mechanical unfolding and dissociation of the GAIN domains of ADGRG1/GPR56, ADGRL1/Latrophilin-1 and ADGRB3/BAI3: insights into the mechanical activation hypothesis of adhesion G protein-coupled receptors

This study examines how mechanical forces affect the GAIN domains of three aGPCRs, showing force-induced destabilization and detachment, supporting the mechanical activation hypothesis.

Mass-immigration shapes the antibiotic resistome of wastewater treatment plants

The paper monitors antibiotic resistance genes in wastewater treatment plants and finds that mass-immigration of bacteria and ARGs from upstream units shapes ARG communities, with stochastic processes dominating their assembly.

A regulatory circuit involving the NADH dehydrogenase-like complex balances C4 photosynthetic carbon flow and cellular redox in maize

This study investigates the role of the NDH complex in maize by analyzing mutants, finding it regulates carbon flow and redox balance in C4 photosynthesis.

Acetylation of fission yeast tropomyosin does not promote differential association with cognate formins

This study investigates whether acetylation of fission yeast tropomyosin affects its binding to formins, finding no preferential association with formin-mediated actin filaments.

Born with intronless ERF transcriptional factors: C4 photosynthesis inherits a legacy dating back 450 million years

This study identifies intronless ERF transcription factors as a molecular tool used in the evolution of C4 photosynthesis in multiple plant lineages.

Detyrosination enrichment on microtubule subsets is established by the interplay between a stochastically-acting enzyme and microtubule stability

The paper investigates how detyrosination of microtubules occurs on specific subsets through stochastic enzyme activity and microtubule stability interactions.

Structures of LIG1 uncover the mechanism of sugar discrimination against a ribonucleotide at 3'- and 5'-end of the nick DNA

This study determines the structures of LIG1/nick DNA complexes with 3- or 5-ribonucleotides to reveal how LIG1 discriminates against ribonucleotides at DNA ends.

Response of the organellar and nuclear (post)transcriptomes of Arabidopsis to drought stress

This study examines (post)transcriptional changes in Arabidopsis under drought stress using long non-coding RNA-sequencing, revealing that alternative splicing significantly affects gene expression and proteome diversity.

Sniff Olfactometer (SO) Protocols

This paper describes protocols for using a sniff olfactometer to determine odorant thresholds by measuring odorant recognition probabilities associated with odorant concentrations.

Increased α-ketoglutarate as a missing link from the C3-C4 intermediate state to C4 photosynthesis in the genus Flaveria

The paper identifies increased α-ketoglutarate (AKG) as a key factor in the transition from C3 to C4 photosynthesis in Flaveria species, supported by metabolomic, sequence, and simulation analyses.

Metformin decreases Cyp26a1 to prevent hepatocarcinogenesis through down- regulating CD8+ T cells

This study shows metformin reduces Cyp26a1 expression, increasing atRA levels and decreasing pro-tumor CD8+ T cells, thereby preventing hepatocarcinogenesis in a mouse model.

Crippled Coronavirus: 5'-PolyU targeted Oligo prevents development of infectious Virions

The paper demonstrates that a 3-polyA oligonucleotide targeting the 5-polyU tract of mouse coronavirus inhibits infectious virion production without affecting host cells.

SARS-CoV-2 remodels the Golgi apparatus to facilitate viral assembly and secretion

This study shows SARS-CoV-2 remodels the Golgi apparatus to facilitate viral assembly and secretion by altering its structure and function.

Structures of LIG1 engaging with mutagenic mismatches inserted by polβ in base excision repair

This study determines the X-ray structures of LIG1 with mismatched DNA substrates to elucidate how LIG1 discriminates between mutagenic and correct mismatches during base excision repair.

Precision engineering of an anti-HLA-A2 chimeric antigen receptor in regulatory T cells for transplant immune tolerance

This paper develops an anti-HLA-A2 CAR for Tregs to induce transplant tolerance, showing they target HLA-A2-expressing grafts and suppress immune rejection.

TNFa and IL-6 promote ex-vivo proliferation of lineage-committed human regulatory T cells

This study shows pro-inflammatory cytokines TNF and IL-6 enhance Treg proliferation without compromising their function or lineage identity.