R. Schekman

Mechanism-guided engineering of a minimal biological particle for genome editing

This study engineers a minimal EDV for genome editing by removing viral components, showing improved efficiency and reduced size.

From Policy to Practice: Tracking an Open Science Funding Initiative

This paper evaluates the effectiveness of the ASAP initiative in improving research output sharing through open science compliance monitoring.

Colocalization of Cancer Associated Biomarkers on Single Extracellular Vesicles for Early Cancer Detection

This paper develops a liquid biopsy assay that detects tumor-specific biomarkers colocalized on extracellular vesicles for early cancer detection.

Two RNA-binding proteins mediate the sorting of miR223 from mitochondria into exosomes

The paper identifies two RNA-binding proteins, YBX1 and YBAP1, that mediate the sorting of miR223 from mitochondria into exosomes.

Syncytin-mediated open-ended membrane tubular connections facilitate the intercellular transfer of cargos including Cas9 protein

The paper demonstrates that syncytin-mediated open-ended membrane tubular connections enable efficient intercellular transfer of Cas9 protein, unlike closed-ended connections or EVs.

Dually targeted proteins regulate proximity between peroxisomes and partner organelles

The paper investigates dually targeted proteins in yeast and shows they act as dynamic tethers between peroxisomes and partner organelles, contributing to their proximity and communication.

Unconventional secretion of α-synuclein mediated by palmitoylated DNAJC5 oligomers

This study identifies DNAJC5 palmitoylation-mediated oligomerization as a mechanism for α-synuclein unconventional secretion across multiple neuronal cell models.

Selective sorting of microRNAs into exosomes by phase-separated YBX1 condensates

The paper shows YBX1 phase separation enables selective sorting of miR-223 into exosomes by recruiting it into condensates that facilitate RNA packaging.

Extracellular vesicles from neuronal cells promote neural induction of mESCs through cyclinD1

The paper shows that extracellular vesicles from neuronal cells promote neural induction of mESCs via cyclinD1, with cyclinD1-enriched EVs enhancing this effect.

Low-bias ncRNA Libraries using Ordered Two-Template Relay: Serial Template Jumping by a Modified Retroelement Reverse Transcriptase

This paper develops OTTR, a low-bias RNA sequencing method using retroelement RTs, outperforming commercial kits and enabling comprehensive ncRNA inventories.

SAR1 paralogs differ biochemically in assembly of the COPII coat

The paper identifies paralog-specific amino acid clusters in SAR1 that affect GTP exchange kinetics and SEC23A binding, revealing functional differences between SAR1A and SAR1B in COPII coat assembly and lipoprotein secretion.

A CLN6-CLN8 complex recruits lysosomal enzymes at the ER for Golgi transfer

The paper identifies CLN6 and the EGRESS complex as essential for lysosomal enzyme trafficking from the ER to the Golgi, revealing their role in Batten disease pathogenesis.

Distinct mechanisms of microRNA sorting into cancer cell-derived extracellular vesicle subtypes

The paper identifies distinct miRNA sorting mechanisms in cancer cell-derived extracellular vesicles, showing selective and non-selective pathways mediated by Lupus La and miR-122 motifs.

New factors for protein transport identified by a genome-wide CRISPRi screen in mammalian cells

The paper identifies two new factors, TTC17 and CCDC157, involved in protein transport through a CRISPRi screen in mammalian cells, showing their roles in Golgi structure and transport carrier fusion.

TANGO1 and SEC12 are co-packaged with procollagen I to facilitate the generation of large COPII carriers

The paper shows TANGO1, cTAGE5, and SEC12 are co-packed with procollagen I into large COPII carriers to facilitate transport of large cargo.

Remodeling of ER-exit sites initiates a membrane supply pathway forautophagosome biogenesis

The paper identifies a pathway where FIP200 and CTAGE5 remodel ER-exit sites to generate COPII vesicles that contribute to autophagosome biogenesis during starvation.

A broad role for YBX1 in defining the small non-coding RNA composition of exosomes

The paper uses TGIRT-seq to analyze RNA in exosomes, revealing that YBX1 influences the sorting of small non-coding RNAs like tRNAs and Y RNAs into exosomes.

Neurodegeneration-associated mutant TREM2 proteins abortively cycle between the ER and ER-Golgi intermediate compartment

This study shows that neurodegeneration-associated TREM2 mutants fail to mature and reach the cell surface, accumulating in the ERGIC and suggesting its role in quality control.

Transparency in Authors’ Contributions and Responsibilities to Promote Integrity in Scientific Publication

The paper advocates for transparent authorship practices in scientific publishing by recommending standardized authorship criteria, CRediT methodology, ORCID identifiers, and institutional and funding agency support.

COPII-coated membranes function as transport carriers of intracellular procollagen-1

The paper demonstrates that large COPII vesicles function as transport carriers for intracellular procollagen-1 using in vivo and in vitro evidence.