X. Tian

Differentiating specific and non-specific protein-metabolite interactions using gradient open port probe electrospray ionization mass spectrometry

This paper introduces a method using gradient open port probe electrospray ionization mass spectrometry to distinguish specific from non-specific protein-metabolite interactions by analyzing charge state distributions under varying methanol concentrations.

Single-cell multiomics reveals a gene regulatory circuit promoting leukemia cell differentiation

This study uses single-cell multiomics to identify a gene regulatory circuit involving SPI1 and CEBPE that promotes differentiation in APL leukemia cells under ATRA treatment.

Establishment of an Inhalation Administration Noninvasive Murine Model for Rapidly Testing Drug Activity against Mycobacterium tuberculosis

This paper develops a non-invasive murine inhalation model using autoluminescent M. tuberculosis to rapidly assess drug efficacy against tuberculosis.

EmbB and EmbC Regulate the Sensitivity of Mycobacterium abscessus to Echinomycin

This study identifies EmbB and EmbC as regulators of Mycobacterium abscessus sensitivity to echinomycin, showing that EmbB mutations can partially compensate for EmbC loss and alter cell wall permeability.

Mutations in ClpC1 or ClpX subunit of the caseinolytic protease confer resistance to natural product ilamycins in mycobacteria

This study identifies two ilamycin analogs targeting mycobacterial Clp protease components and discovers mutations conferring resistance to these compounds.

A CRISPR-Nonhomologous End-Joining-based strategy for rapid and efficient gene disruption in Mycobacterium abscessus

This paper introduces a CRISPR-NHEJ strategy for efficient gene disruption in Mycobacterium abscessus and shows that homologous recombination and single-strand annealing components are required for NHEJ repair.

Targeting transcription factors through an IMiD independent zinc finger domain

This study identifies a new IMiD-independent drug targeting SALL4's ZFC4 domain, leading to significant tumor growth inhibition in vivo.

Lithocholic acid phenocopies rejuvenating and life-extending effects of calorie restriction

This study identifies lithocholic acid as a metabolite that mimics calorie restriction's anti-aging effects in multiple species through AMPK activation.

Lithocholic acid targets TULP3 to activate sirtuins and AMPK to retard ageing

This study identifies LCA's mechanism of action through TULP3-sirtuin-v-ATPase-AMPK pathway to activate AMPK and extend lifespan in model organisms.

Linked regulation of genome integrity and senescence-associated inflammation by p53

This study identifies a mitochondria-regulated p53 circuit that links genomic instability and inflammation in senescent cells, showing p53 suppresses inflammation without affecting cell cycle arrest.

Discovery and biological evaluation of a potent small molecule CRM1 inhibitor for its selective ablation of extranodal NK/T cell lymphoma

This study identifies and evaluates LFS-1107, a CRM1 inhibitor that suppresses ENKTL cell growth by blocking NF-κB signaling and shows promise for clinical translation.

Local two-photon excitation reveals the mechanism of mitochondrial Ca2+ release

This study uses two-photon excitation to reveal that mitochondrial Ca2+ release is driven by ATP synthesis and the electron transport chain, not Ca2+ entry.

Regulation of Lung Immune Tone by the Gut-Lung Axis via Dietary Fiber, Gut Microbiota, and Short-Chain Fatty Acids

This study shows that dietary fiber and gut microbiota influence lung immune tone via short-chain fatty acids, reducing inflammation and lung injury in mice.

O-GlcNAcylation of Keratin 18 coordinates TCA cycle to promote cholangiocarcinoma progression

The paper shows O-GlcNAcylation of Keratin 18 coordinates TCA cycle enzymes to promote cholangiocarcinoma progression by stabilizing K18 and altering metabolite levels.

AMPK targets PDZD8 to trigger carbon source shift to glutamine

This paper identifies AMPK's role in shifting carbon utilization from glucose to glutamine via PDZD8 phosphorylation, enhancing glutaminolysis and extending lifespan in C. elegans and mice.

SALLB not targeted by IMiDs is important for maintenance of liver cancer cells

The paper identifies a compound that degrades both SALL4 isoforms, overcoming IMiD limitations in liver cancer treatment.

SeMOE allows for quantitative glycan perception and exhibits anti-cancer potentiality

SeMOE enables absolute glycan quantification and visualization through selenosugar-based metabolic oligosaccharide engineering, with demonstrated anti-cancer potential.

DNA repair and anti-cancer mechanisms in the longest-living mammal: the bowhead whale

This study investigates DNA repair mechanisms in bowhead whales, showing their high efficiency and accuracy in repairing DNA damage, which may explain their long lifespan without cancer.

Increased hyaluronan by naked mole-rat HAS2 extends lifespan in mice

This study shows that overexpressing the naked mole-rat HAS2 gene in mice increases hyaluronan levels, reduces inflammation, and extends lifespan and healthspan.

Artificially cultivated duckweed: a high-efficiency starch producer

This paper reports a method to produce starch efficiently from duckweed under specific conditions and identifies mechanisms behind its high starch accumulation.

Duckweed evolution: from land back to water

This study investigates the reverse evolution of duckweed from land back to water, showing gradual degeneration in root structure, stomatal function, and lignocellulose content, along with gene pathway changes.

Expansion microscopy with trypsin digestion and tyramide signal amplification (TT-ExM) for protein and lipid staining

The paper introduces TT-ExM, an improved expansion microscopy technique that reduces protein loss and enhances fluorescence signals through trypsin digestion and tyramide signal amplification, enabling ultrafine-resolution imaging of cellular structures.

RelB represses miR-193a-5p expression to promote the phenotypic transformation of vascular smooth muscle cells in aortic aneurysm

This study identifies miR-193a-5p as a regulator of vascular smooth muscle cell proliferation and migration in aortic aneurysms, showing that RelB represses its expression.

PTPN1 deficiency modulates BMPR2 signaling and induces endothelial dysfunction in Pulmonary Arterial Hypertension

This study identifies PTPN1 as a regulator of BMPR2 signaling in PAH, showing its downregulation in PAH patients and link to endothelial dysfunction.

Pathogen Clearance-based Correlates of Immunoprotection Reveal Insightful Features of Vaccine Immunity

This paper introduces a new vaccine evaluation system based on pathogen clearance and liver trapping to assess immunoprotective potential against bacterial infections.

Potent Bacterial Vaccines Require FcγRIIB-mediated Pathogen Capture by Liver Sinusoidal Endothelium

This paper identifies FcγRIIB-mediated capture of antibody-coated bacteria by liver sinusoidal endothelial cells as a mechanism for superior vaccine efficacy.

NSUN2-mediated mRNA m5C Modification Regulates the Progression of Hepatocellular Carcinoma

This study identifies NSUN2-mediated m5C modification as a regulator of hepatocellular carcinoma progression through oncogene expression and Ras signaling pathway modulation.

An Engineered Amber-Emitting Nano Luciferase and Its Use for Immunobioluminescence Imaging in Vivo

The paper develops a red-shifted NanoLuc variant (QLuc) and uses it for in vivo immunobioluminescence imaging to improve deep-tissue visualization in mice.

Powdery mildew effectors AVRA1 and BEC1016 target the ER J-domain protein HvERdj3B required for immunity in barley

A potent SARS-CoV-2 antibody neutralizes Omicron variant by disassembling the spike trimer

This paper identifies a potent SARS-CoV-2 antibody, 553-49, that neutralizes the Omicron variant by targeting a conserved epitope and disassembling spike trimers.