J. Singh

Discordance in Genotypic and Phenotypic anti-tuberculosis drug susceptibility results: time to reconsider critical concentration.

The paper identifies phenotypic and genotypic discordance in rifampicin resistance of Mycobacterium tuberculosis isolates, showing 15% of isolates misclassified as susceptible despite low-level resistance mutations.

Drp1-JNK knockdown mitigates Scribble loss induced cell proliferation, metastasis and lethality phenotypes in Drosophila

This study shows that reducing Drp1 activity in Scrib loss models decreases cell proliferation, metastasis, and lethality by restoring cell polarity and mitochondrial function.

Scribble knockdown induced metastasis tracking, identification of its associated novel molecular candidates through Proteome and in silico studies

This study shows Scribble knockdown in Drosophila leads to metastasis and identifies novel proteins involved in the process.

PPARγ mediated enhanced lipid biogenesis fuels Mycobacterium tuberculosis growth in a drug-tolerant hepatocyte environment.

This study shows Mtb induces PPARγ in hepatocytes to create lipid droplets and drug-tolerant environments, contributing to systemic pathogenicity and metabolic impairments during TB infection.

Single-cell morphodynamical trajectories enable prediction of gene expression accompanying cell state change

The paper develops MMIST, a computational approach linking live-cell morphodynamics to gene expression changes during cell state transitions.

Translation initiation or elongation inhibition triggers contrasting effects on Caenorhabditis elegans survival during pathogen infection

This study shows that inhibiting translation initiation in C. elegans increases survival during P. aeruginosa infection, while inhibiting elongation decreases survival, due to distinct immune responses.

Genomes of Aegilops umbellulata provide new insights into unique structural variations and genetic diversity in the U-genome for wheat improvement

This study presents a high-quality genome assembly of Ae. umbellulata and resequencing data revealing structural variations, evolutionary sub-populations, and new resistance gene haplotypes useful for wheat improvement.

Inhibition of the UFD-1-NPL-4 complex triggers an aberrant immune response in Caenorhabditis elegans

This study shows that inhibiting the UFD-1-NPL-4 complex in C. elegans triggers an aberrant immune response that harms immunocompetent worms but benefits immunocompromised ones.

The Expression of HIV-1 Tat in Lactococcus lactis

This study optimizes the expression of HIV-1 Tat in Lactococcus lactis, showing functional transactivation of the HIV-1 promoter.

Mechanism for Vipp1 spiral formation, ring biogenesis and membrane repair

The paper investigates how Vipp1 polymerizes into planar sheets and spirals on membranes, revealing geometric transitions that may inform ESCRT-III filament behavior.

Identifying Selectivity Filters in Protein Biosensor for Ligand Screening

The paper identifies selectivity filters in MopR, a bacterial phenol biosensor, that screen ligands based on chemical features and size, revealing conserved mechanisms for ligand selection.

Calcineurin inhibition enhances Caenorhabditis elegans lifespan by defecation defects-mediated calorie restriction and nuclear hormone signaling

This study shows calcineurin inhibition in C. elegans extends lifespan by causing defecation defects that mimic calorie restriction and activate nuclear hormone signaling.

A DNA Unwinding Equilibrium Serves as a Checkpoint for CRISPR-Cas12a Target Discrimination

This study investigates Cas12a's target discrimination mechanism, revealing a DNA unwinding equilibrium as a checkpoint for mismatch sensing before cleavage.

PI(3,5)P2 Controls the Signaling Activity of Class I PI3K

This study identifies PI(3,5)P2 as a regulator of Class I PI3K activity by interacting with its p85 subunit, revealing a feedback mechanism that impacts cancer and tissue repair.

Activity-based profiling of cullin-RING ligase networks by conformation-specific probes

The paper develops conformation-specific probes to profile activated cullin-RING ligase networks, revealing differential regulation across cell types and activation pathways.

Two RNA-binding proteins mediate the sorting of miR223 from mitochondria into exosomes

The paper identifies two RNA-binding proteins, YBX1 and YBAP1, that mediate the sorting of miR223 from mitochondria into exosomes.

Intranasal self-amplifying RNA SARS-CoV-2 vaccine produces protective respiratory and systemic immunity and prevents viral transmission

This study compares intranasal and intramuscular delivery of a self-amplifying RNA SARS-CoV-2 vaccine, showing that intranasal delivery induces mucosal immunity and reduces viral transmission.

Ancestral SARS-CoV-2 driven antibody repertoire diversity in an unvaccinated individual correlates with expanded neutralization breadth

The paper examines antibody diversity in an unvaccinated SARS-CoV-2 infected individual, finding that post-vaccination neutralization breadth against Omicron variants is comparable to vaccinated individuals and breakthrough infections.

A non-destructive approach for measuring rice panicle-level photosynthetic responses using 3D-image reconstruction

This paper develops a non-destructive method to measure rice panicle-level photosynthetic responses using 3D imaging and gas exchange analysis, revealing differential responses to heat stress between two rice genotypes.

Phenol Sensing in Nature Modulated via a Conformational Switch Governed by Dynamic Allostery

The paper shows that MopR, an NtrC protein, uses dynamic allostery to sense phenol, altering its conformation and ATPase activity, with implications for evolutionary conservation.

A self-amplifying RNA vaccine against COVID-19 with long-term room-temperature stability

This paper develops a self-amplifying RNA vaccine against SARS-CoV-2 with room-temperature stability and strong immune responses in preclinical studies.

Insights into the role of Nup62 and Nup93 in assembling cytoplasmic ring and central transport channel of the nuclear pore complex

This study investigates the role of Nup62 and Nup93 in NPC assembly, revealing their interactions and structural contributions to the cytoplasmic ring and central transport channel.

Cross-linking of the Endolysosomal System Reveals Flotillin Structures and Putative Cargo

The paper uses cross-linking mass spectrometry to identify protein structures and cargo associated with lysosomes and early endosomes, revealing tetrameric PPT1 and flotillin complexes.

Presence of sodium taurocholate co-transporting polypeptide and Hepatitis B replication marker on placenta: Another home for the virus

The paper investigates NTCP and HBV replication markers in placental cells, finding NTCP expression and viral markers suggesting placental susceptibility to HBV and potential replication.

A combination of potently neutralizing monoclonal antibodies isolated from an Indian convalescent donor protects against the SARS-CoV-2 delta variant

This paper identifies two potent monoclonal antibodies from an Indian donor that neutralize SARS-CoV-2 variants including Delta, showing protection in a mouse model.

Dithiothreitol causes toxicity in C. elegans by modulating the methionine-homocysteine cycle

This study shows DTT toxicity in C. elegans is modulated by the methionine-homocysteine cycle and depends on the IRE-1/XBP-1 pathway.

Cryo-EM structure of human Nup155 reveals the biochemical basis for atrial fibrillation linked genetic mutation R391H

This paper presents the cryo-EM structure of human Nup155 at 5.2 Å resolution, revealing structural features and the impact of the R391H mutation on NPC function and atrial fibrillation.

Decoding the Mechanism of Specific RNA Targeting by Ribosomal Methytransferases

This study determines the structure of 30S-KsgA and identifies factors guiding its specificity, enabling strategies to reverse methylation-mediated antibiotic resistance.

In-vitro Reconstitution of Membrane Contact Sites between the Endoplasmic Reticulum and Lipid Droplets

This study reconstitutes MCS between ER and lipid droplets in vitro, showing Rab18 and phosphatidic acid regulate MCS formation in response to metabolic and immune signals.

Possible link between higher transmissibility of B.1.617 and B.1.1.7 variants of SARS-CoV-2 and increased structural stability of its spike protein and hACE2 affinity

This study uses molecular dynamics simulations to compare the structural stability and binding affinity of SARS-CoV-2 Spike-ACE2 complexes from wild-type and two variants, finding increased binding energy and stability in variants B.1.1.7 and B.1.617.