A. Clark

Interferon-Inducible Guanylate-Binding Protein 5 Inhibits Replication of Multiple Viruses by Binding to the Oligosaccharyltransferase Complex and Inhibiting Glycoprotein Maturation

This paper identifies GBP5 as an ISG that inhibits viral replication by blocking N-glycosylation of viral proteins, including SARS-CoV-2 spike, through interaction with the OST complex.

Defining focal neuroendocrine differentiation as a transcriptionally distinct form of prostate cancer pathology characterized by the expression of androgen receptors

This study uses single-cell RNA sequencing to show that focal neuroendocrine differentiation (Focal NED) in prostate cancer is transcriptionally distinct, characterized by androgen receptor expression and unique transcriptional sub-populations.

Electrophysiological characterisation of iPSC-derived human β-like cells and an SLC30A8 disease model.

This paper uses electrophysiological tools to functionally characterize iPSC-derived human β-like cells and assess the impact of a SLC30A8 variant on β-cell electrical coupling.

Rapid evolution of piRNA clusters in the Drosophila melanogaster ovary

This study investigates the evolution of piRNA clusters in Drosophila melanogaster by comparing piC activity and sequence composition across strains, supporting both de novo and trap models of piC formation.

High-resolution glucose tracing and in situ imaging reveals regulated lactate production in human pancreatic β cells

The paper uses metabolic tracing and imaging to show that human pancreatic β cells produce lactate, regulated by LDHB expression, which affects Ca2+ spiking without altering ATP/ADP levels.

Integrating carbon stocks and wildlife connectivity for nature-based climate solutions

The paper identifies areas in Ontario that maximize carbon storage and terrestrial connectivity, finding weak positive correlation and highlighting gaps in protected areas coverage.

The genomic landscape of Saccharomyces paradoxus introgression in geographically diverse Saccharomyces cerevisiae strains

The paper identifies introgression from Saccharomyces paradoxus into Saccharomyces cerevisiae genomes using a hidden Markov model, finding widespread introgression with varying amounts and locations.

Interactions of SARS-CoV-2 envelope protein with amilorides correlate with antiviral activity

This study investigates the structure of SARS-CoV-2 E protein and its interactions with amilorides, finding that binding affinity correlates with antiviral activity and that residue Asn15 is critical for maintaining the binding site conformation.

Variation in self-compatibility among genotypes and across ontogeny in a self-fertilizing vertebrate

The paper examines variation in self-compatibility and fecundity among mangrove rivulus genotypes and across ontogeny, showing lineage differences in sex change influence these traits.

CGRP-receptor family reveals endogenous GPCR agonist bias and its significance in primary human cardiovascular cells

The paper demonstrates endogenous agonist bias at the calcitonin-like receptor in primary human cardiovascular cells, showing physiological consequences through RAMP switching.

Multi-Clonal Live SARS-CoV-2 In Vitro Neutralization by Antibodies Isolated from Severe COVID-19 Convalescent Donors

This study identifies multi-clonal neutralizing antibodies against SARS-CoV-2 in severe COVID-19 patients and shows their potential for combination therapy.

Emergence of stable motifs in consumer-resource communities

The paper shows that vertical trophic chains emerge as stable and efficient structures in consumer-resource communities under minimal conditions.

A High Content Screen for Mucin-1-Reducing Compounds Identifies Fostamatinib as a Candidate for Rapid Repurposing for Acute Lung Injury during the COVID-19 pandemic

The paper identifies Fostamatinib as a potential repurposing drug for acute lung injury by reducing MUC1 protein levels in preclinical models.

Single Molecule Light Field Microscopy

This paper introduces SMLFM, a 3D single molecule localization technique with 20 nm precision, and demonstrates its application to image fixed eukaryotic cell membranes below the diffraction limit.

Structure and mechanism of potent bifunctional β-lactam- and homoserine lactone-degrading enzymes from marine microorganisms

This study investigates the structure and mechanism of two marine-derived β-lactamase-lactonase enzymes, revealing their structural conservation and diverse reaction mechanisms.

Laboratory Analysis of an Outbreak of Candida auris in New York from 2016 to 2018-Impact and Lessons Learned

This study analyzes a C. auris outbreak in New York, identifying factors contributing to its spread and demonstrating the effectiveness of new detection methods.

The ARRIVE guidelines 2019: updated guidelines for reporting animal research

The paper updates the ARRIVE guidelines for reporting animal research, introducing an Essential 10 checklist and a Recommended Set to improve transparency and reproducibility.

Reporting animal research: Explanation and Elaboration for the ARRIVE guidelines 2019

This paper explains and elaborates on the revised ARRIVE guidelines for reporting animal research, providing rationale and examples to improve transparency and reproducibility.

An Open-Source Plate Reader

The paper describes the design and benchmarking of an open-source microplate reader with spectrophotometric and fluorescence detection capabilities.

Polysaccharide-mediated synthesis of melanins from serotonin and other 5-hydroxy indoles

The paper investigates polysaccharide-mediated oxidation of serotonin and 5-hydroxy indoles into melanin-like materials, showing one product affects cytokine release from immune cells.