C.-H. Wu

Comparison of Multiple Carbapenemase Tests Based on an Unbiased Colony Selection Method

This study compares carbapenemase tests using an unbiased colony selection method, finding improved detection and highlighting performance discrepancies among tests.

Development of a tightly regulated copper-inducible transient gene expression system in Nicotiana benthamiana incorporating suicide exon and Cre recombinase

This paper develops a copper-inducible gene expression system in Nicotiana benthamiana to tightly regulate transgene expression and cell death assays.

MASCOT-Skyline integrates population and migration dynamics to enhance phylogeographic reconstructions

MASCOT-Skyline jointly infers spatial and temporal transmission dynamics of infectious diseases using a structured coalescent skyline approach, revealing biases in existing phylogeographic methods.

Activation of plant immunity through conversion of a helper NLR homodimer into a resistosome

This study shows how the helper NLR NRC2 converts from a homodimer to a higher-order oligomer upon activation by the sensor NLR Rx, revealing the molecular mechanism of plant immune activation.

A root-specific NLR network confers resistance to plant parasitic nematodes

The paper identifies a root-specific NLR gene cluster in tomato that confers resistance to nematodes and evolved from an ancient NRC network.

Functional divergence shaped the network architecture of plant immune receptors

The paper shows how functional divergence in NRC3 variants shapes the genetic architecture of the NLR network in solanaceous plants, leading to subfunctionalization and increased immune system complexity.

NRC immune receptor networks show diversified hierarchical genetic architecture across plant lineages

The paper investigates the evolution of NRC immune receptor networks across plant lineages, finding significant expansion and complexity in lamiids compared to Ericales and campanulids.

The NRC0 gene cluster of sensor and helper NLR immune receptors is functionally conserved across asterid plants

The paper investigates the functional conservation of the NRC0 gene cluster across asterid plants and shows its role in immune responses.

Resurrection of plant disease resistance proteins via helper NLR bioengineering

A helper NLR is mutated to evade inhibition by a parasite effector, restoring disease resistance gene activity.

Sensor NLR immune proteins activate oligomerization of their NRC helper

The paper shows that activation of plant NLRs Rx and Bs2 leads to oligomerization of the helper NLR NRC2, suggesting a distinct activation model compared to mammalian paired NLRs.

A cell cycle-linked mechanism for the glutamine driven establishment of stem cell fate

This study identifies a mechanism by which mitochondrial glutamine metabolism links cell cycle progression to stem cell fate decisions through PASK and WDR5 interactions.

An atypical NLR protein modulates the NRC immune receptor network

The paper identifies NRCX, an atypical NRC family member, and shows it modulates the NRC immune receptor network by affecting plant growth and hypersensitive responses.

The helper NLR immune protein NRC3 mediates the hypersensitive cell death caused by the cell-surface receptor Cf-4

The paper identifies NRC3 as an NLR protein required for hypersensitive cell death triggered by the PRR Cf-4 in response to the fungal effector Avr4.

Rpi-amr3 confers resistance to multiple Phytophthora species by recognizing a conserved RXLR effector

The paper identifies Rpi-amr3 as a resistance gene that recognizes a conserved RXLR effector from multiple Phytophthora species, conferring broad resistance.

Dynamic accumulation of a helper NLR at the plant-pathogen interface underpins pathogen recognition

This study shows that the NLR protein NRC4 dynamically changes its subcellular localization during plant-pathogen interaction, accumulating at the haustorium interface before activation and redistributing after immune recognition.

Plant pathogens convergently evolved to counteract redundant nodes of an NLR immune receptor network

The paper identifies effectors from plant pathogens that specifically target subsets of NRC immune receptor proteins, revealing convergent strategies to counteract redundant nodes in the NLR network.

Global spread and evolutionary history of HCV subtype 3a

The paper uses host and virus genomic data to trace the global spread and evolutionary history of HCV subtype 3a, finding it originated in South Asia and spread through three independent events post-World War II.

Bacillus subtilis YngB contributes to wall teichoic acid glucosylation and glycolipid formation during anaerobic growth

This study identifies YngB as a UGPase in B. subtilis that contributes to WTA glucosylation and glycolipid formation under anaerobic conditions.

A complex resistance locus in Solanum americanum recognizes a conserved Phytophthora effector

The paper identifies a new R gene, Rpi-amr1, from Solanum americanum that provides broad-spectrum resistance to Phytophthora infestans and defines its allelic diversity.

Relative adrenal insufficiency is a risk factor and an endotype of sepsis - A proof of concept study to support a precision medicine approach for glucocorticoid sepsis therapy

This study establishes a relative adrenal insufficiency (RAI) animal model and shows that glucocorticoid (GC) therapy benefits RAI mice but harms non-RAI mice, supporting a precision medicine approach for sepsis treatment.

The Two Amino Acids Proximate to the Renin Cleavage Site of Human Angiotensinogen Do Not Affect Angiotensin II-mediated Functions in Mice

This study shows that replacing amino acids 11 and 12 in mouse angiotensinogen with human counterparts does not affect renin cleavage or angiotensin II-mediated functions in mice.

A CRISPR/Cas9 mediated 53 kb deletion of the NRC4 gene cluster of tomato does not affect bacterial flagellin-triggered immunity

This study deletes the NRC4 gene cluster in tomato using CRISPR/Cas9 and finds it does not affect flg22-induced immunity.

An N-terminal motif in NLR immune receptors is functionally conserved across distantly related plant species

The paper identifies a conserved N-terminal motif in plant NLR immune receptors and shows its functional conservation across species.

A metagene of NRF2 expression is a prognostic biomarker in all stage colorectal cancer

The paper defines an NRF2 metagene and shows it predicts poor prognosis in all stages of colorectal cancer, with higher expression in CMS4 subtype.

Image-based consensus molecular subtype classification (imCMS) of colorectal cancer using deep learning

This paper develops imCMS, a deep learning method to classify colorectal cancer subtypes from histological images, showing improved accuracy over bioinformatic methods.

Tomato Prf requires NLR helpers NRC2 and NRC3 to confer resistance against the bacterial speck pathogen Pseudomonas syringae pv. tomato

BEAST 2.5: An Advanced Software Platform for Bayesian Evolutionary Analysis

The paper describes new developments in the BEAST 2.5 software platform for Bayesian evolutionary analysis, expanding joint inference over multiple data types and complex phylodynamics.

High resolution evolutionary analysis of within-host hepatitis C virus infection

This study analyzes within-host HCV evolution in HIV-coinfected individuals, finding population structure, regional variation in mutation rates, and a low recombination rate.

The coming of age of EvoMPMI: evolutionary molecular plant-microbe interactions across multiple timescales

This paper reviews recent advances in EvoMPMI, highlighting new systems and findings that bridge mechanistic molecular research with evolutionary approaches in plant-microbe interactions.

Lessons in effector and NLR biology of plant-microbe systems

The paper examines three effectors from the rice blast fungus and their corresponding rice NLR immune receptors to highlight general concepts of plant-microbe interactions and identify 12 lessons in effector and NLR biology.