F. Klein

Dissecting the impact of somatic hypermutation on SARS-CoV-2 neutralization and viral escape

The paper reverts mutations in SARS-CoV-2 antibodies to study SHM's impact on neutralization, finding that some mutations are critical for neutralizing Omicron variants.

Mapping the neutralizing specificity of human anti-HIV serum by deep mutational scanning

This study maps the neutralizing specificity of human anti-HIV serum using deep mutational scanning to identify Env mutations affecting neutralization by polyclonal sera.

Combined multidimensional single-cell protein and RNA profiling dissects the cellular and functional heterogeneity of thymic epithelial cells

The paper uses combined single-cell protein and RNA profiling to identify and characterize novel subpopulations of thymic epithelial cells and their functional roles.

Neutralization sensitivity of the SARS-CoV-2 Omicron BA.2.75 sublineage

This study evaluates the neutralization sensitivity of the SARS-CoV-2 BA.2.75 sublineage compared to other variants, finding it less resistant to antibodies than BA.4/5 but more than BA.2.

Probabilities of HIV-1 bNAb development in healthy and chronically infected individuals

The paper uses sequence analysis to predict the likelihood of HIV-1 broadly neutralizing antibody (bNAb) development in healthy and infected individuals, finding that chronic infection is not required for bNAb generation.

The unique ORF8 protein from SARS-CoV-2 binds to human dendritic cells and induces a hyper-inflammatory cytokine storm

This study shows that SARS-CoV-2's ORF8 protein binds to human dendritic cells, induces cytokine storms, and may be a target for therapeutic intervention.

Delineating antibody escape from Omicron sublineages

This study evaluates antibody escape from Omicron sublineages by testing serum and monoclonal antibody activity against BA.1, BA.2, BA.2.12.1, and BA.4/5, revealing increased resistance in BA.4/5 and identifying promising neutralizing antibodies.

A naturally arising broad and potent CD4-binding site antibody with low somatic mutation

This paper describes a VRC01-class antibody with lower somatic mutation but comparable breadth and potency to other broadly neutralizing antibodies against HIV-1.

No substantial pre-existing B cell immunity against SARS-CoV-2 in healthy adults

The paper analyzes B cell immunity in unexposed adults and finds no substantial pre-existing antibody or B cell response to SARS-CoV-2.

Intranasal administration of a monoclonal neutralizing antibody protects mice against SARS-CoV-2 infection

This paper describes a monoclonal antibody, DZIF-10c, that effectively neutralizes SARS-CoV-2 and its variants when administered intranasally to mice, offering potential for topical treatment.

A FOXN1 mutation competitively displaces wild-type FOXN1 from higher order nuclear condensates to cause immunodeficiency

This paper identifies a FOXN1 mutation that displaces wild-type FOXN1 from nuclear condensates, leading to immunodeficiency and athymia in heterozygotes.

Kinetics and correlates of the neutralizing antibody response to SARS-CoV-2

This study examines the kinetics and correlates of the SARS-CoV-2 neutralizing antibody response in 963 recovered individuals over 10 months, finding variations in antibody persistence and identifying factors predicting neutralizing activity.

A novel humanized mouse model to study mucosal HIV-1 transmission and prevention

This paper introduces a novel humanized mouse model, CD34T+, which is highly permissive to mucosal HIV-1 infection and can be prevented by broadly neutralizing antibodies.

Predicting in vivo escape dynamics of HIV-1 from a broadly neutralizing antibody

The paper models HIV-1 escape dynamics from broadly neutralizing antibodies using clinical trial data, identifying fitness factors that predict viral reproduction rates and resistance tradeoffs.

Longitudinal isolation of potent near-germline SARS-CoV-2-neutralizing antibodies from COVID-19 patients

This study isolates potent SARS-CoV-2-neutralizing antibodies from COVID-19 patients as early as 8 days post-diagnosis and identifies precursor B cells in healthy individuals.

SARS-CoV-2 targets cortical neurons of 3D human brain organoids and shows neurodegeneration-like effects

This study shows SARS-CoV-2 infects cortical neurons in 3D human brain organoids, causing neurodegeneration-like effects such as Tau mislocalization and neuronal death.

openPrimeR for multiplex amplification of highly diverse templates

The paper introduces openPrimeR, an R-based tool for designing multiplex PCR primers for highly diverse templates, and demonstrates its effectiveness in amplifying immunoglobulins.

De novo Classification of Mouse B Cell Types using Surfaceome Proteotype Maps

The paper develops autoCSC, a miniaturized surfaceome mapping technology, to classify mouse B cell types and identify developmental subpopulations.

Meiosis-specific prophase-like pathway controls cleavage-independent release of cohesin by Wapl phosphorylation

The paper identifies a cleavage-independent pathway for cohesin release during meiotic prophase-I in budding yeast, regulated by phosphorylation of Rec8 and Wapl by PLK and DDK.

The SIRAH force field 2.0: Altius, Fortius, Citius

The paper introduces an updated coarse-grained SIRAH force field for proteins, demonstrating its ability to accurately model structural and dynamic behaviors of calcium-binding proteins like Calmodulin.