H. Suga

Targeting the Plasmodium falciparum UCHL3 ubiquitin hydrolase using chemically constrained peptides

This study identifies chemically constrained peptides that inhibit PfUCHL3, a critical deubiquitinating enzyme in Plasmodium falciparum, by blocking substrate binding rather than active site interaction.

DNA methylation enables recurrent endogenization of giant viruses in an animal relative

The paper shows that DNA methylation in Amoebidium appalachense silences endogenized giant viruses, contributing 14% of its genes and revealing a dynamic process of viral integration and purging.

A cyclic peptide toolkit reveals mechanistic principles of peptidylarginine deiminase IV (PADI4) regulation

The paper develops cyclic peptide modulators of PADI4 and reveals their mechanism of action and cellular activity.

Genome-resolved meta-omics unveils rock-hosted lifestyle of enigmatic DPANN archaea

This study investigates the lifestyle of DPANN archaea in rock-hosted environments using genome-resolved meta-omics, revealing a symbiotic lifestyle and ecological interactions with bacteria and minerals.

mRNA display reveals a class of high-affinity bromodomain-binding motifs that are not found in the human proteome

The paper uses mRNA display to identify high-affinity bromodomain-binding motifs not present in the human proteome, revealing novel binding modes and suggesting evolutionary pressure against strong binders.

Discovery and characterization of cyclic peptides selective for the C-terminal bromodomains of BET family proteins

The paper discovers and characterizes cyclic peptides that selectively bind to the C-terminal bromodomains of BET family proteins, showing high potency and paralogue-specificity.

Accurate models of substrate preferences of post-translational modification enzymes from a combination of mRNA display and deep learning

The paper develops a platform combining mRNA display and deep learning to accurately model substrate preferences of PTM enzymes, showing high accuracy and complexity in enzyme specificity.

Potent macrocycle inhibitors of the human SAGA deubiquitinating module

The paper identifies potent cyclic peptide inhibitors of the USP22 subunit in the SAGA DUB module, showing increased H2B monoubiquitination in treated cells.

De novo macrocyclic peptides for inhibiting, stabilising and probing the function of the Retromer endosomal trafficking complex

The paper develops macrocyclic peptides that inhibit, stabilize, and probe the Retromer complex's function, showing high affinity and specificity for different subunits.

Human trans-editing enzyme displays tRNA acceptor stem specificity and relaxed amino acid selectivity

This study examines how human ProXp-ala recognizes tRNA acceptor stem elements and amino acids, showing specificity changes compared to bacterial enzymes.

Promiscuous enzymes cooperate at the substrate level en route to lactazole A

This study investigates the biosynthesis of lactazole A, revealing dynamic enzyme cooperation and kinetic control in thiopeptide assembly using an in vitro system.

State-selective Modulation of Heterotrimeric Gαs Signaling with Macrocyclic Peptides

This study identifies two macrocyclic peptides that selectively modulate active and inactive states of Gs, offering a new approach for developing state-dependent GTPase inhibitors.

Cyclic peptides can engage a single binding pocket through multiple, entirely divergent modes

The paper identifies high-affinity, specific cyclic peptide inhibitors of BET bromodomains using RaPID screening, revealing diverse binding modes and structures.

Minimal lactazole scaffold for in vitro production of pseudo-natural thiopeptides

This paper develops a platform for in vitro biosynthesis of lactazole A, enabling the creation of diverse thiopeptide analogs with modified structures.

A unicellular relative of animals generates an epithelium-like cell layer by actomyosin-dependent cellularization

The paper studies cellularization in Sphaeroforma arctica, showing it forms an epithelium-like layer via actomyosin-dependent membrane invaginations and regulated gene activation.