T. Friedrich

Effective in vivo binding energy landscape illustrates kinetic stability of RBPJ-DNA binding

The paper investigates RBPJ-DNA binding kinetics and cofactor interactions, finding that kinetic stability, not thermodynamic, governs target site specificity and that mutations affect both association and dissociation rates.

CD8+ cells and small viral reservoirs facilitate post-ART control of SIV in Mauritian cynomolgus macaques

Nanocontainer derived from silkworm carotenoprotein for carotenoid extraction and presentation in biotechnology and biomedical applications

This study characterizes the structure and function of BmCBP, a silkworm carotenoprotein, showing its ability to bind and deliver carotenoids for biomedical applications.

The structure, binding, and function of a Notch transcription complex involving RBPJ and the epigenetic reader protein L3MBTL3

This paper defines the RBPJ-interacting domain of L3MBTL3 and reports the crystal structure of the RBPJ-L3MBTL3-DNA complex, revealing molecular interactions and functional consequences of mutations.

Limited within-host diversity and tight transmission bottlenecks limit SARS-CoV-2 evolution in acutely infected individuals

The paper analyzes SARS-CoV-2 within-host diversity and transmission bottlenecks in acute infections, finding limited diversity and narrow bottlenecks that restrict variant spread.

Early embryonic loss following intravaginal Zika virus challenge in rhesus macaques

This study infects rhesus macaques intravaginally with Zika virus and finds that two of three pregnancies resulted in early embryonic loss with viral RNA detected at the maternal-fetal interface.

Transmission of SARS-CoV-2 in domestic cats imposes a narrow bottleneck

This study shows SARS-CoV-2 in cats has limited within-host genetic variation and a narrow transmission bottleneck, with a variant H655Y emerging and spreading across transmission pairs.

Mass Photometry of Membrane Proteins

This study uses mass photometry to analyze membrane proteins and mimetic systems, revealing structural insights and improving nanodisc assembly characterization.

Quantifying the heterogeneity of macromolecular machines by mass photometry

This paper demonstrates that mass photometry accurately quantifies sample heterogeneity with high resolution and minimal volume, outperforming negative stain electron microscopy in detecting proteasome assemblies.

Primary infection with dengue or Zika virus does not affect the severity of heterologous secondary infection in macaques.

This study investigates whether prior dengue or Zika virus infection affects the severity of subsequent heterologous secondary infections in macaques, finding no significant impact on clinical outcomes.

Zika viruses of both African and Asian lineages cause fetal harm in a vertical transmission model

This study shows African ZIKV can cause fetal harm in a mouse model, challenging the idea that specific mutations are required for congenital Zika syndrome.

Pre-existing SIV Infection Increases Susceptibility to Tuberculosis in Mauritian Cynomolgus Macaques

This study shows that pre-existing SIV infection in macaques increases susceptibility to tuberculosis by impairing immune control of M.tb.

Radioprotective role of cyanobacterial phycobilisomes

The paper investigates the radioprotective role of cyanobacterial phycobilisomes by exposing Synechocystis sp. PCC6803 to ionizing radiation and space flight, revealing their potential role in stress response and recovery.

Doxorubicin induces caspase-mediated proteolysis of KV7.1

This study identifies caspase-mediated proteolysis of Kv7.1 as a novel mechanism regulated by doxorubicin, leading to reduced cardiac potassium currents.

Functional interaction of low-homology FRPs from different cyanobacteria with Synechocystis OCP

The paper investigates the functional interaction of low-homology FRPs from different cyanobacteria with Synechocystis OCP, revealing distinct binding properties despite similar structural conformations.

Infection via mosquito bite alters Zika virus replication kinetics in rhesus macaques

This study compares ZIKV replication kinetics and tissue distribution in rhesus macaques infected via mosquito bite versus subcutaneous inoculation, finding delayed viral peak and restricted tissue distribution in mosquito-infected animals.

The photocycle of orange carotenoid protein conceals distinct intermediates and asynchronous changes in the carotenoid and protein components

This study identifies two novel photocycle intermediates in the Orange Carotenoid Protein (OCP) using time-resolved spectroscopy, revealing asynchronous changes in carotenoid and protein components.

Disruption Of Ankyrin B And Caveolin-1 Interaction Sites Alters Na+,K+-ATPase Lateral Diffusion In HEK293 Cell Plasma Membranes

This study examines how disrupting Na+,K+-ATPase interactions with ankyrin B and caveolin-1 affects its lateral diffusion in HEK293 cells using fluorescence techniques.

The Unique Protein-To-Protein Carotenoid Transfer Mechanism

The paper demonstrates a unique protein-to-protein carotenoid transfer mechanism using COCP to assemble photoactive OCP from photoinactive species.

Deletion of the short N-terminal extension in OCP reveals the main site for the FRP binding

The paper identifies the main site of OCP-FRP interactions by analyzing structural and functional similarities between the NTE and FRP.

Assembly of photoactive Orange Carotenoid Protein from its domains unravels a carotenoid shuttle mechanism

The study reassembles the Orange Carotenoid Protein (OCP) from its domains, revealing a carotenoid shuttle mechanism where the CTD binds carotenoid and transfers it to the NTD.