A. J. Borst

Atomically accurate de novo design of antibodies with RFdiffusion

This paper designs antibodies de novo with atomic precision using RFdiffusion and yeast display, validated by cryo-EM and biophysical methods.

De novo design of allosterically switchable protein assemblies

This paper designs allosterically switchable protein assemblies using rigid-body coupling of effector-switchable modules, demonstrating cooperative ligand binding and structural changes similar to natural systems.

Local structural flexibility drives oligomorphism in computationally designed protein assemblies

This study shows that structural flexibility in computationally designed protein assemblies leads to multiple architectures, expanding their functional possibilities.

Design of four component T=4 tetrahedral, octahedral, and icosahedral protein nanocages through programmed symmetry breaking

The paper designs T=4 protein nanocages with tetrahedral, octahedral, and icosahedral symmetries using pseudo-symmetrization of trimeric building blocks, resulting in structures with distinct subunits and interfaces.

Blueprinting expandable nanomaterials with standardized protein building blocks

This paper introduces standardized protein building blocks and geometric design principles to create expandable protein nanomaterials, validated through X-ray crystallography and electron microscopy.

Computational design of non-porous, pH-responsive antibody nanoparticles

This paper designs pH-responsive antibody nanoparticles with three distinct protein components that disassemble at tunable pH levels, enabling targeted delivery of biologics.

Precisely patterned nanofibers made from extendable protein multiplexes

The paper designs extendable protein multiplexes with symmetric architectures to create precisely patterned nanofibers through controlled repeat propagation and hydrophilic patch placement.

Improving the secretion of designed protein assemblies through negative design of cryptic transmembrane domains

This study identifies cryptic transmembrane domains in designed protein nanoparticles that hinder secretion and introduces a computational tool, Degreaser, to remove them while maintaining stability, improving secretion efficiency.

Immunization with a self-assembling nanoparticle vaccine displaying EBV gH/gL protects humanized mice against lethal viral challenge

This study evaluates a nanoparticle vaccine displaying EBV gH/gL, showing it protects humanized mice against lethal EBV challenge, while monomeric gH/gL does not.

Structure-based design of stabilized recombinant influenza neuraminidase tetramers

This study designs stabilized recombinant influenza neuraminidase tetramers by uncovering structural principles and improving thermal stability and antibody affinity.

Vitrification after multiple rounds of sample application and blotting improves particle density on cryo-electron microscopy grids.

This paper describes a method to improve particle density on cryo-EM grids through multiple rounds of sample application and blotting, enhancing structural studies of various biomolecules.