J. Snijder

Structural basis for recognition of the FLAG-tag by anti-FLAG M2

The paper determines the atomic structure of the FLAG peptide bound to anti-FLAG M2 Fab, identifies key interactions, and proposes a shorter FLAG-tag variant with equivalent binding.

Reverse engineering the anti-MUC1 hybridoma antibody 139H2 by mass spectrometry-based de novo sequencing

This paper reverse engineers the anti-MUC1 hybridoma antibody 139H2 using mass spectrometry-based sequencing and validates its properties through various assays.

Direct mass spectrometry-based detection and antibody sequencing of Monoclonal Gammopathy of Undetermined Significance from patient serum - a case study.

This paper detects MGUS using LC-MS and determines M-protein sequences from serum, providing molecular detail for monoclonal gammopathies.

Sialoglycan binding triggers spike opening in a human coronavirus

This study shows that sialoglycan binding triggers the opening of the spike protein in the human coronavirus HKU1 through allosteric inter-domain cross-talk.

Contemporary human H3N2 influenza A viruses require a low threshold of suitable glycan receptors for efficient infection

This study shows that recent H3N2 influenza A viruses require a low threshold of glycan receptors for efficient infection, and increasing receptor numbers beyond this threshold does not improve infection efficiency.

A broad-spectrum macrocyclic peptide inhibitor of the SARS-CoV-2 spike protein

This paper discovers a macrocyclic peptide that inhibits SARS-CoV-2 and related sarbecoviruses by targeting a conserved site in the spike protein, offering a potential broad-spectrum antiviral.

Spectral Interferences Impede the High-Resolution Mass Analysis of Recombinant Adeno-Associated Viruses

The paper characterizes recombinant adeno-associated viruses (rAAVs) using native mass spectrometry, revealing spectral interferences caused by heterogeneity and charging behavior.

Glycan shield of the ebolavirus envelope glycoprotein GP

This study profiles the site-specific glycosylation patterns of ebolavirus GP using mass spectrometry, revealing complex N-, O-, and C-linked glycans that contribute to the glycan shield and restrict antibody neutralization.

Assessment of genome packaging in AAVs using Orbitrap-based charge detection mass spectrometry

This paper introduces a method using Orbitrap-based charge detection mass spectrometry to rapidly and sensitively quantify AAV genome packaging, improving bioprocessing and drug development.

Frequency chasing of individual megadalton ions in an Orbitrap analyzer improves precision of analysis in single molecule mass spectrometry

This study improves mass spectrometry precision by tracking ion frequency drift in high-mass ions, achieving better resolution and sampling through desolvation correction.

Structure-based design of a highly stable, covalently-linked SARS-CoV-2 spike trimer with improved structural properties and immunogenicity

This paper designs a modified SARS-CoV-2 spike trimer, VFLIP, with enhanced stability, immunogenicity, and structural fidelity to the native spike.

Mass spectrometry-based sequencing of the anti-FLAG-M2 antibody using multiple proteases and a dual fragmentation scheme

This paper introduces a method for de novo sequencing of monoclonal antibodies using multiple proteases and dual fragmentation, achieving high accuracy in sequence coverage.

Adeno-associated virus capsid assembly is divergent and stochastic

This study reveals that AAV capsid assembly is highly stochastic and heterogeneous, with each capsid having a unique composition.

Cryo-EM structure of coronavirus-HKU1 haemagglutinin esterase reveals architectural changes arising from prolonged circulation in humans

The paper presents the cryo-EM structure of HKU1 HE, revealing structural changes due to prolonged human circulation, including a truncated lectin domain and intact esterase domain.

Resolving heterogeneous high-mass macromolecular machineries by Orbitrap-based single particle charge detection mass spectrometry

This paper demonstrates that Orbitrap-based single particle charge detection mass spectrometry can resolve high-mass heterogeneous biomolecular assemblies by accurately predicting charge states and masses.

Vitrification after multiple rounds of sample application and blotting improves particle density on cryo-electron microscopy grids.

This paper describes a method to improve particle density on cryo-EM grids through multiple rounds of sample application and blotting, enhancing structural studies of various biomolecules.