E. Villa

Cryo-electron tomography reveals the microtubule-bound form of inactive LRRK2

This study uses cryo-ET to show that LRRK2 can form microtubule-bound filaments in its autoinhibited state, with structural differences compared to active-LRRK2 filaments.

Asesino: a nucleus-forming phage that lacks PhuZ

The paper studies a phage lacking PhuZ, showing it replicates efficiently without this protein, highlighting variation in nucleus-based replication pathways.

The Molecular Architecture of the Nuclear Basket

This study uses structural biology to reveal the architecture of the nuclear basket in yeast and mammals, showing how Mlp/Tpr proteins form its structural components.

An essential and highly selective protein import pathway encoded by nucleus-forming phage

This paper identifies components of a novel protein import pathway in phages, revealing a mechanism for selective protein trafficking into the phage nucleus.

Integrative mapping reveals molecular features underlying the mechanism of nucleocytoplasmic transport

The paper constructs an integrative Brownian dynamics model of nucleocytoplasmic transport, revealing how FG repeat proteins and nuclear transport receptors mediate selective transport through NPCs.

A mobile intron facilitates interference competition between co-infecting viruses

The paper shows a mobile intron in bacteriophage ΦPA3 uses its homing endonuclease to interfere with co-infecting phage ΦKZ, providing a competitive reproductive advantage.

A transcriptionally active lipid vesicle encloses theinjected Chimalliviridae genome in early infection

The paper shows that Chimalliviridae form a lipid membrane-bound EPI vesicle during early infection, which hosts transcription of the phage genome, and demonstrates the necessity of a second compartment, the phage nucleus, for genome replication.

Identification of the bacteriophage nucleus protein interaction network

The paper identifies proteins interacting with the phage nucleus protein ChmA and describes their potential roles in genome packaging and transport.

Identifying the core genome of the nucleus-forming bacteriophage family and characterization of Erwinia phage RAY

The paper identifies a core genome of nucleus-forming bacteriophages and characterizes the replication mechanism of Erwinia phage RAY.

Architecture and self-assembly of the jumbo bacteriophage nuclear shell

The paper identifies chimallin as the primary protein forming the bacteriophage nuclear shell and characterizes its self-assembly into closed compartments.

Comprehensive Structure and Functional Adaptations of the Yeast Nuclear Pore Complex

This study presents the structure of the yeast nuclear pore complex using cryo-EM, revealing its functional adaptations and evolutionary links.

A Cytoskeletal Vortex Drives Phage Nucleus Rotation During Jumbo Phage Replication inE. coli

The paper identifies a cytoskeletal vortex in E. coli jumbo phage Goslar that drives phage nucleus rotation, similar to eukaryotic structures.

Entropy Regularized Deconvolution of Cellular Cryo-Transmission Electron Tomograms

The paper applies entropy regularized deconvolution to cryo-ET data to improve resolution and reduce noise, assessing results via Fourier analysis and subtomogram analysis.

Subcellular Organization of Viral Particles During Maturation of Nucleus-Forming Jumbo Phage

The paper visualizes the subcellular organization of mature viral particles in nucleus-forming jumbo phages, revealing a structured assembly process called phage bouquets.

A modular platform for engineering function ofnatural and synthetic biomolecular condensates

The paper identifies design principles for biomolecular condensates using PopZ and applies them to create synthetic condensates in human cells.

Selective transport of fluorescent proteins into the phage nucleus

The paper investigates nuclear transport in phage infection systems, finding that GFPmut1 can be selectively transported into the {Phi}KZ phage nucleus, with specific amino acid residues identified for this process.

The SARS-CoV-2 Nucleocapsid phosphoprotein forms mutually exclusive condensates with RNA and the membrane-associated M protein

This study shows the SARS-CoV-2 N protein forms distinct condensates with RNA and M protein, aiding viral RNA packaging and virion assembly.

Asymmetric localization of the cell division machinery during Bacillus subtilis sporulation

This study shows that during Bacillus subtilis sporulation, the cell division machinery is asymmetrically localized, leading to different cell fates.

TDP-43 and HSP70 phase separate into anisotropic, intranuclear liquid spherical annuli

The paper shows acetylation of TDP-43 drives its phase separation into intranuclear spherical annuli with liquid properties, regulated by HSP70 chaperone activity and proteasome function.

Parkinson's Disease-linked LRRK2 structure and model for microtubule interaction

This study reports the structure of LRRK2 and its interaction with microtubules, showing that its conformation regulates binding and affects motor protein function.

The in situ structure of Parkinson’s disease-linked LRRK2

This study uses cryo-electron tomography to reveal the in situ structure of LRRK2 with a pathogenic mutation, showing its oligomerization around microtubules and functional domains.

The molecular architecture of engulfment during Bacillus subtilis sporulation

This study uses cryo-electron tomography to visualize Bacillus subtilis sporulation, revealing new insights into the engulfment process and the role of the SpoIIDMP complex in membrane migration.