E. Appleton

IFNB induced by non-lytic virus immunotherapy promotes improved survival in hepatocellular carcinoma, mediated by MHCII-independent cytotoxic CD4+ T-cells

This paper shows that uv-inactivated reovirus combined with sorafenib improves survival in HCC by promoting IFNβ-induced cytotoxic CD4+ T-cell responses that are MHCII-independent.

PARP inhibitors enhance reovirus-mediated cell killing through the death-inducing signaling complex (DISC) with an associated NF-kappa B-regulated immune response.

This paper identifies PARP inhibitors as sensitizers for oncolytic reovirus by enhancing DISC-mediated apoptosis and NF-kappa B-driven immune responses.

Optimising a self-assembling peptide hydrogel as a Matrigel alternative for 3-dimensional mammary epithelial cell culture

This study optimizes self-assembling peptide hydrogels to better support mammary epithelial cell organoid formation compared to Matrigel.

CDK4/6 inhibition and dsRNA sensor agonism co-operate to enhance anti-cancer effects through ER stress and immune modulation of tumour cells

Novel Laser Capture Microdissection-Proteomic Analysis Identifies Spatially Distinct Extracellular Matrix Signatures in the Core and Infiltrating Edge of Human Glioblastoma.

This paper develops an LCM-proteomics protocol to analyze spatial ECM differences in human glioblastoma, identifying distinct matrisome components between tumor epicenter and brain/tumor interface.

Single-cell level temporal profiling of tumour-reactive T cells under immune checkpoint blockade

This study uses a novel mouse model to analyze how checkpoint blockade affects tumour-reactive T cell activation dynamics at the single-cell level.

Iterative transcription factor screening enables rapid generation of microglia-like cells from human iPSC

This study uses iterative transcription factor screening to generate microglia-like cells from human iPSCs, identifying a combination of TFs that efficiently differentiate iPSCs into microglia-like cells within 4 days.

Senescence inhibits the chaperone response to thermal stress

The paper shows that senescence impairs the chaperone response to thermal stress in human mesenchymal stem cells, leading to misfolded protein accumulation.