J. Swift

Collagen fibril formation at the plasma membrane occurs independently from collagen secretion

The paper investigates collagen fibril formation in fibroblasts, showing it occurs at the plasma membrane independently of secretion and involves endocytosis.

Single nuclei sequencing reveals C4 photosynthesis is based on rewiring of ancestral cell identity networks

The paper uses single nuclei sequencing to show C4 photosynthesis evolves through rewiring of cell identity networks from C3 plants.

Optimising a self-assembling peptide hydrogel as a Matrigel alternative for 3-dimensional mammary epithelial cell culture

This study optimizes self-assembling peptide hydrogels to better support mammary epithelial cell organoid formation compared to Matrigel.

Stress Recovery Triggers Rapid Transcriptional Reprogramming and Activation of Immunity in Plants

This study shows that drought recovery in plants rapidly activates immune responses, enhancing pathogen resistance across multiple tomato species.

Arabidopsis transcriptome responses to low water potential using high throughput plate assays

The paper compares transcriptional responses in Arabidopsis to different water potential reduction methods and introduces a high-throughput hard agar assay.

Loss of cytoskeletal proteostasis links dysregulation of cell size and mechanotransduction in mesenchymal stem cell senescence

This study shows that loss of cytoskeletal proteostasis in senescent mesenchymal stem cells disrupts mechanotransduction and matrix protein secretion, linking cell size dysregulation to tissue maintenance issues.

Novel Laser Capture Microdissection-Proteomic Analysis Identifies Spatially Distinct Extracellular Matrix Signatures in the Core and Infiltrating Edge of Human Glioblastoma.

This paper develops an LCM-proteomics protocol to analyze spatial ECM differences in human glioblastoma, identifying distinct matrisome components between tumor epicenter and brain/tumor interface.

Vimentin intermediate filaments provide structural stability to the mammalian Golgi apparatus

This study shows vimentin intermediate filaments support Golgi structural stability, with GORAB mediating this interaction, affecting Golgi disassembly and reassembly dynamics.

Mmp14 is required for matrisome homeostasis and circadian rhythm in fibroblasts

This study shows that Mmp14 regulates circadian rhythms and matrisome homeostasis in tendon fibroblasts by controlling collagen fibril synthesis and crosslinking.

Senescence inhibits the chaperone response to thermal stress

The paper shows that senescence impairs the chaperone response to thermal stress in human mesenchymal stem cells, leading to misfolded protein accumulation.

BioID based proteomic analysis of the Bid interactome identifies novel proteins involved in cell cycle dependent apoptotic priming.

The paper uses BioID to identify novel Bid interactors involved in cell cycle-dependent apoptotic priming, highlighting VDAC2's role in Bid phosphorylation and sensitivity to anti-mitotic drugs.

Laser capture microdissection coupled mass spectrometry (LCM-MS) for spatially resolved analysis of formalin-fixed and stained human lung tissues

This paper develops an optimized microproteomics method for analyzing H&E-stained FFPE lung tissues, identifying 1252 differentially expressed proteins including 137 ECM proteins.

Estrogen mediates acute elastic fibre homeostasis in skin

This study shows that estrogen deficiency reduces skin elasticity and elastic fibre abundance, which can be reversed by estrogen replacement.

Stimulation of cardiac fibroblast Piezo1 channels opposes myofibroblast differentiation and induces IL-6 secretion via Ca2+-mediated p38 MAP kinase activation

This study shows that activating Piezo1 channels in cardiac fibroblasts reduces myofibroblast differentiation and increases IL-6 secretion through Ca2+-dependent p38 MAP kinase activation.

Circadian control of the secretory pathway is a central mechanism in tissue homeostasis

The paper identifies a circadian mechanism regulating collagen synthesis, assembly, and degradation to maintain tissue homeostasis in mice.

Distinct matrix composition and mechanics in aged and estrogen-deficient mouse skin

This study examines how aging and estrogen deficiency affect skin matrix composition and mechanics in mice, finding contrasting effects on tensile strength and collagen levels.

A Novel Scavenging Tool for Cancer Biomarker Discovery based on the Blood-Circulating Nanoparticle Protein Corona

This paper uses lipid-based nanoparticles to capture and analyze tumor-specific proteins in blood, identifying potential cancer biomarkers through mass spectrometry.

microRNA-dependent regulation of biomechanical genes establishes tissue stiffness homeostasis

The paper identifies microRNA-dependent regulation of biomechanical genes that maintain tissue stiffness homeostasis through cytoskeletal and extracellular matrix control.

Nuclear decoupling is part of a rapid protein-level cellular response to high-intensity mechanical loading

The paper investigates how human mesenchymal stem cells respond to mechanical loading by decoupling nuclear and cytoskeletal mechano-transmission at the protein level.

An immortalised mesenchymal stem cell line maintains mechano-responsive behaviour and can be used as a reporter of substrate stiffness

The paper shows that an immortalized mesenchymal stem cell line retains mechano-responsiveness and can serve as a reporter for substrate stiffness.