M. Donowitz

Host-derived CEACAM-laden vesicles engage enterotoxigenic E. coli for elimination and toxin neutralization.

The paper shows that host-derived CEACAM-laden vesicles capture ETEC and its toxins, reducing pathogen load and neutralizing toxins during infection.

Disruption of Epithelium Integrity by Inflammation-Associated Fibroblasts through Prostaglandin Signaling

This study shows inflammation-associated fibroblasts disrupt colon epithelial barrier integrity and genome stability via PGE2-EP4 signaling, and EP4 inhibitors can reverse these effects.

Loss of PI3-kinase activity of inositol polyphosphate multikinase impairs PDK1-mediated AKT activation, cell migration, and intestinal homeostasis

This study shows that IPMK's PI3-kinase activity is essential for PDK1 membrane localization, which activates AKT and supports intestinal homeostasis and regeneration.

Air-Liquid-Interface Reorganizes Membrane Lipid and Enhance Recruitment of Slc26a3 to Lipid-Rich Domains in Human Colon

The paper shows that air-liquid interface culture increases DRA expression and activity in Caco-2/BBe cells and colonoids by enhancing ceramide-rich platform formation and differentiation.

SLC26A3 (DRA) is stimulated in a synergistic, intracellular Ca2+ dependent manner by cAMP AND ATP in intestinal epithelial cells

The paper shows that forskolin and ATP synergistically increase DRA activity in intestinal epithelial cells through intracellular Ca2+ elevation, which is blocked by BAPTA-AM.

Adipokine C1q/Tumor necrosis factor-related protein 3 (CTRP3) Attenuates Intestinal Inflammation via Histone Deacetylase Sirtuin 1 (SIRT1)/NF-κB Signaling

This study shows that CTRP3 reduces intestinal inflammation in colitis models and IBD patients by modulating SIRT1/NF-κB signaling to suppress pro-inflammatory cytokines.

SARS-COV-2 induced Diarrhea is inflammatory, Ca2+ Dependent and involves activation of calcium activated Cl channels

This study investigates the pathophysiology of SARS-CoV-2-induced diarrhea, showing it is inflammatory, calcium-dependent, and involves activation of calcium-activated chloride channels.

Chronic exposure of ErbB TKIs inhibits DRA expression and activity through an ERK/Elk-1/CREB/AP-1 dependent pathway

Human Rotavirus Diarrhea Is Associated with Altered Trafficking and Expression of Apical Membrane Transport Proteins

Mechanical Stimuli Affect E. coli Heat Stable Enterotoxin-cyclic GMP Signaling in a Human Enteroid Intestine-Chip Diarrhea Model

This study shows that fluid flow in an Intestine-Chip model alters intestinal epithelial responses to E. coli heat-stable enterotoxin, increasing cGMP secretion without enhancing intracellular cGMP.

Organoid-derived Duodenum Intestine-Chip for preclinical drug assessment in a human relevant system

The paper develops a human Duodenum Intestine-Chip model to better predict drug metabolism and drug-drug interactions by emulating intestinal functions and CYP450 induction.

Epithelial WNT2B and Desert Hedgehog are necessary for human colonoid regeneration after bacterial cytotoxin injury

cAMP stimulates SLC26A3 activity in human colon by a CFTR-dependent mechanism that does not require CFTR activity