T. Nishimura

Arrayed CRISPRi library to suppress genes required for Schizosaccharomyces pombe viability

The paper constructs CRISPRi libraries to knock down essential genes in S. pombe, revealing metabolic pathway interactions and enabling functional studies of essential genes.

Label-free imaging of intracellular structures in living mammalian cells via external apodization phase-contrast microscopy

This study introduces ExAPC microscopy for label-free visualization of intracellular structures, showing its ability to resolve biomolecular condensates, lipid droplets, and mitochondria with high spatiotemporal resolution.

Condensation of PRPP amidotransferase by phase separation promotes de novo purine synthesis through intermolecular intermediate channeling in yeast

This study shows that PRPP amidotransferase forms condensates in yeast under purine deprivation, enhancing de novo purine synthesis through intermolecular intermediate channeling.

Cell polarity linked to gravity sensing is generated by protein translocation from statoliths to the plasma membrane.

This study identifies LAZY1-LIKE proteins as gravity sensors in plant statocytes, showing their polar localization based on gravity direction.

Comprehensive analysis of autophagic functions of WIPI family proteins and their implications for the pathogenesis of β-propeller associated neurodegeneration

This study investigates the roles of WIPI proteins in autophagy and their implications in β-propeller associated neurodegeneration, finding that WIPI2 is essential for autophagy and that some BPAN-related mutations affect autophagic activity.

Unique Amphipathic α-helix Drives Membrane Insertion and Enzymatic Activity of ATG3

The paper identifies an amphipathic α-helix in ATG3 that facilitates membrane insertion and enzymatic activity essential for autophagosome formation.

Cis-membrane association of human ATG8 proteins N-terminus mediates autophagy

This study shows that the N-termini of lipidated ATG8 proteins associate with membranes in cis, which is essential for autophagosome expansion and efficient p62 degradation.

Actin filaments couple the protrusive tips to the nucleus through the I-BAR domain protein IRSp53 for migration of elongated cells on 1D fibers

This study shows that IRSp53 couples actin filaments to the nucleus via membrane curvature during 3D cell migration on 1D fibers, affecting protrusion and contractility.

Engineering of an electrically charged hydrogel implanted into a traumatic brain injury model for stepwise neuronal tissue reconstruction

The paper describes a two-step method using charged hydrogels and neural stem cell injection to promote brain tissue reconstruction after traumatic injury.

HSD11β1 promotes EMT-mediated breast cancer metastasis

This study identifies HSD11β1 as a promoter of breast cancer metastasis via EMT induction and shows that targeting HSD11β1 could suppress metastasis.

The polyol pathway is an evolutionarily conserved system for sensing glucose uptake

The paper shows the polyol pathway senses glucose uptake in flies and mice, enabling metabolic remodeling through ChREBP/MondoA activation.

Translation of cellular protein localization by generative adversarial network

This study uses a generative adversarial network to translate actin filament images into protein localization images, showing high similarity for actin-related proteins but lower for microtubules.

Ubiquitin ligase RIPLET mediates polyubiquitination of RIG-I and LGP2 and regulates the innate immune responses to SARS-CoV-2 infection

This study identifies RIPLET as a ubiquitin ligase that activates RIG-I signaling and modulates innate immune responses to SARS-CoV-2 by polyubiquitinating RIG-I and LGP2.

MCM10 compensates for Myc-induced DNA replication stress in breast cancer stem-like cells

This study shows that MCM10 compensates for c-Myc-induced DNA replication stress in breast cancer stem-like cells, promoting genome duplication and drug resistance.

Involvement of I-BAR protein IRSp53 in tumor cell growth via extracellular microvesicle secretion

This study shows IRSp53 promotes tumor cell proliferation through microvesicle secretion, as evidenced by reduced secretion and phosphorylation in knockout cells and increased survival in IRSp53-deficient mice.

A flexible target-specific anti-infection therapeutic platform that can be applied to different microbial species

This paper introduces a photoimmuno-antimicrobial strategy (PIAS) that effectively targets and eliminates various pathogens, including drug-resistant strains, without harming host microflora.

The polyol pathway is a crucial glucose sensor in Drosophila

This study identifies the polyol pathway as a glucose sensor in Drosophila that activates the metabolic regulator Mondo, linking glucose levels to transcriptional responses.

Safety assessment of a novel C-type natriuretic peptide derivative and the mechanism of bone- and cartilage-specific toxicity

This study assesses the safety of ASB20123, a C-type natriuretic peptide derivative, showing bone- and cartilage-specific toxicity linked to growth acceleration and identifying epiphyseal plate thickness and BMD as biomarkers.

The RhoGEF Cysts couples apical polarity proteins to Rho and myosin activity at adherens junctions

This study identifies Cysts as a key regulator linking apical polarity proteins to Rho1 and myosin activity at adherens junctions in Drosophila.