A. Tan

Evolving a High-Performance Terminal Deoxynucleotidyl Transferase for Enzymatic DNA Synthesis

This paper evolves a terminal deoxynucleotidyl transferase for enzymatic DNA synthesis using modified substrates, showing improved activity, template promiscuity, and thermostability.

Spatial transcriptomics reveals ovarian cancer subclones with distinct tumour microenvironments

This study uses spatial and single-cell transcriptomics to identify ovarian cancer subclones with distinct microenvironments and infiltration patterns linked to treatment response.

Multiple performance peaks for scale-biting in an adaptive radiation of pupfishes

The paper analyzes feeding kinematics in pupfishes to identify performance peaks associated with adaptation to a novel trophic niche, using deep learning for automated motion tracking.

Comparison of tet(X4)-containing contigs from metagenomic sequencing data with plasmid sequences of isolates from a cohort of healthy subjects.

This study compares tet(X4)-containing contigs from metagenomic data with plasmid sequences from isolates in a healthy cohort, finding high similarity and co-occurrence of resistance genes.

The Host-specific Microbiota is Required for Diet-Specific Metabolic Homeostasis

This study shows that host-specific microbiota are essential for maintaining diet-specific metabolic homeostasis, while foreign microbiota impair metabolic fitness in germ-free mice.

A transcriptome-wide meta-analysis reveals lack of cancer-cell intrinsic determinants of response to immune checkpoint blockade

This study performs a transcriptome-wide meta-analysis to identify determinants of immune checkpoint blockade response, finding that stromal factors, not cancer-cell intrinsic features, are key predictors across tumor types.

nELISA: A high-throughput, high-plex platform enables quantitative profiling of the inflammatory secretome

nELISA enables high-throughput, high-plex protein profiling of the inflammatory secretome, revealing 447 significant cytokine responses across multiple donors and conditions.

Using photographs and deep neural networks to understand flowering phenology and diversity in mountain meadows

This study uses deep learning on crowd-sourced images to quantify flowering species richness in alpine meadows, finding higher richness near the tree line and demonstrating the effectiveness of Mask R-CNN for flower detection.

Structural insights into HIV-1 polyanion-dependent capsid lattice formation revealed by singleparticle cryo-EM

This study uses cryo-EM to determine high-resolution structures of HIV-1 capsid lattices containing hexamers and pentamers, revealing the role of IP6 in pentamer formation and the structure of the lattice bound to the drug GS-6207.

Two structural switches in HIV-1 capsid regulate capsid curvature and host factor binding

This study identifies two structural switches in HIV-1 capsid protein that modulate host factor binding based on capsid curvature and oligomerization state.

Innate-adaptive IFN cross-talk reprograms myeloid and T cells for antitumor immunity with a second-generation recombinant MVA

This paper describes a second-generation recombinant MVA vaccine that enhances antitumor immunity by reprogramming myeloid and T cells through innate-adaptive IFN cross-talk.

Exploring the in-situ evolution of Nitrofurantoin resistance in clinically derived Uropathogenic Escherichia coli isolates.

This study investigates the in situ evolution of Nitrofurantoin resistance in uropathogenic E. coli isolates from two UTI patients, finding no fitness advantage for resistant strains but a selective advantage at sub-MIC concentrations.

Gut Microbes and the Liver Circadian Clock Partition Glucose and Lipid Metabolism

This study shows the liver circadian clock and gut microbes interact to regulate glucose and lipid metabolism in mice, with the liver clock driving rhythmic gene expression and gut microbes providing secondary cues when the liver clock is dysfunctional.

Vaccinia E5 is a major inhibitor of the DNA sensor cGAS

This study identifies Vaccinia E5 as a major inhibitor of the cGAS-STING pathway by promoting cGAS degradation through proteasome-dependent ubiquitination.

Inactivation of the core cheVAWY chemotaxis genes disrupts chemotactic motility and organised biofilm formation in Campylobacter jejuni

Immature HIV-1 assembles from Gag dimers leaving partial hexamers at lattice edges as substrates for proteolytic maturation.

This paper investigates the structure and dynamics of immature HIV-1 Gag lattice edges, revealing partial hexamers and their role in proteolytic maturation.

Fungal symbionts produce prostaglandin E2 to promote their intestinal colonization

This study shows that Candida albicans produces prostaglandin E2 to enhance its gut colonization by reducing phagocyte-mediated killing.