J. Diao

N-acetylation of α-synuclein enhances synaptic vesicle clustering mediated by α-synuclein and lysophosphatidylcholine

This study shows that N-acetylation of α-synuclein enhances its ability to cluster synaptic vesicles via increased interaction with lysophosphatidylcholine.

Quantifying cell viability through organelle ratiometric probing

This paper introduces a fluorescent probe, PCV-1, and an analytical assay called organelle ratiometric probing (ORP) to quantify cell viability at the single-cell level using super-resolution microscopy.

Enhancing Mitochondrial Functions by Optogenetic Clustering

This paper develops an optogenetic method to control mitochondrial clustering and shows it enhances mitochondrial functions.

Super-resolution analyzing spatial organization of lysosomes with an organic fluorescent probe

The paper uses super-resolution microscopy and a fluorescent probe to analyze lysosome spatial organization relative to mitochondria, revealing changes in distance distribution patterns during autophagy and linking lysosome positioning to mitochondrial health.

Novel inhibitors of E. coli lipoprotein diacylglyceryl transferase are insensitive to resistance caused by lpp deletion

The paper identifies Lgt inhibitors that remain effective against E. coli even when lpp is deleted, showing Lgt is a robust antibacterial target.

Double-transmembrane domain of SNAREs decelerates the fusion by increasing the protein-lipid mismatch

This study shows that double transmembrane domains in SNAREs slow membrane fusion by increasing protein-lipid mismatch, using in vitro experiments and theoretical analysis.

Mechanism of duplex unwinding by coronavirus nsp13 helicases

This paper investigates the unwinding mechanism of coronavirus nsp13 helicases using single-molecule FRET, revealing distinct step sizes and NTP hydrolysis patterns between MERS-CoV and SARS-CoV-2 nsp13.

Membrane association of VAMP2 SNARE motif in cells and its regulation by different lipid phases of synaptic vesicle membrane

This study investigates how lipid phases in synaptic vesicle membranes regulate the structure and function of the VAMP2 SNARE motif using in-cell NMR and lipidomic profiling.

Quantitative Analysis of Interactive Behavior of Mitochondria and Lysosomes Using Structured Illumination Microscopy

The paper introduces an M-value to quantify mitochondria-lysosome contact and mitophagy using structured illumination microscopy, finding distinct M-value ranges for these processes.

Super-Resolution Tracking of Mitochondrial Dynamics with An Iridium(III) Luminophore

The paper develops an iridium(III) dye for tracking mitochondrial dynamics under structured illumination microscopy, revealing new insights into mitochondria-lysosome interactions.