X. Shao

ChIP-MS reveals the local chromatin composition by label-free quantitative proteomics

The paper introduces ChIP-MS, a method combining ChIP with label-free quantitative mass spectrometry to analyze local chromatin composition, demonstrating its application at telomeres, with transcription factors, in tissue, and via dCas9-driven enrichment.

A therapeutic hepatitis B mRNA vaccine with strong immunogenicity and persistent virological suppression

This paper develops an mRNA vaccine for chronic hepatitis B that shows strong immunogenicity and persistent virological suppression in mouse models.

Proliferative Exhausted CD8 T Cells Exacerbate Long-lasting Anti-tumor Effects in Human Papillomavirus Positive Head and Neck Squamous Cell Carcinoma

The paper identifies a proliferative exhausted CD8+ T cell cluster in HPV-positive HNSCC that correlates with better patient survival and suggests potential therapeutic implications.

Automated container-less cell processing method for single-cell proteomics

The paper develops an automated container-less cell processing platform using acoustic levitation to improve single-cell proteomics throughput and reproducibility by minimizing sample loss and reducing manual steps.

Time-lapsed proteomics reveals a role for the novel protein, SNED1, in modulating ECM composition and protein folding

This paper introduces a time-lapsed proteomic method to study ECM dynamics and identifies SNED1's role in modulating ECM composition and protein folding.

Controllable drug release of pH-sensitive liposomes encapsulating artificial cytosol system

This paper develops an artificial cytosol system using agarose-sucrose to create pH-sensitive liposomes that enhance drug delivery efficiency.

Novel enzyme for dimethyl sulfide-releasing in bacteria reveals a missing route in the marine sulfur cycle

The paper identifies a novel ATP-dependent DMSP lysis pathway in bacteria, revealing a new route in the marine sulfur cycle through the enzyme AcoD.

Neonicotinoids stimulate H2-limited methane emission in Periplaneta americana through the regulation of gut bacterium community

This study shows neonicotinoids increase methane emission in Periplaneta americana by altering gut bacteria to produce more hydrogen, which methanogens use to generate methane.

Single-cell immune landscape of human recurrent spontaneous abortion

This study uses single-cell RNA-sequencing to analyze decidual and peripheral leukocytes in normal and RSA pregnancies, revealing immune dysregulation in RSA.

Genome-wide effects of the antimicrobial peptide apidaecin on translation termination

This study shows that the antimicrobial peptide apidaecin inhibits translation by arresting ribosomes at stop codons and causing stop codon bypass, leading to C-terminal extensions on proteins.

CTLA-4 blockade reverses the Foxp3+ T-regulatory-cell suppression of anti-tuberculosis T-cell effector responses

This study shows that blocking CTLA-4 reverses Treg cell suppression of anti-TB T-cell responses in tuberculosis patients.

Experimental study of tissue-engineered urethra with collagen / chitosan composite as scaffolds

The paper investigates tissue-engineered urethra using collagen/chitosan scaffolds and reports a new method for quantifying epithelial cell activity.

Quantitative Analysis of Interactive Behavior of Mitochondria and Lysosomes Using Structured Illumination Microscopy

The paper introduces an M-value to quantify mitochondria-lysosome contact and mitophagy using structured illumination microscopy, finding distinct M-value ranges for these processes.

Super-Resolution Tracking of Mitochondrial Dynamics with An Iridium(III) Luminophore

The paper develops an iridium(III) dye for tracking mitochondrial dynamics under structured illumination microscopy, revealing new insights into mitochondria-lysosome interactions.