P. Digard

An emerging PB2-627 polymorphism increases the pandemic potential of avian influenza virus by breaking through ANP32 host restriction in mammalian and avian hosts

This paper identifies a PB2-627V mutation in avian influenza viruses that enhances pandemic potential by overcoming host restriction through ANP32A proteins in both avian and mammalian hosts.

Understanding off-target growth defects introduced to influenza A virus by synonymous recoding

This study identifies compensatory mutations that cause replication defects in a CpG-enriched influenza A virus, revealing polymerase slippage and type I interferon induction as key mechanisms.

Novel technology to develop non-transmissible live influenza virus vaccines through alterations in M2/M42 ion channel expression

This paper develops non-transmissible live influenza virus vaccines by altering M2/M42 ion channel expression, showing they protect against H5 HPAIV without transmission.

One for all - Human kidney Caki-1 cells are highly susceptible to infection with corona- and other respiratory viruses

This paper identifies Caki-1 human kidney cells as a highly susceptible model for studying multiple respiratory viruses, including coronaviruses and influenza, offering a versatile platform for cross-virus comparison.

Zoonotic avian influenza viruses evade human BTN3A3 restriction

This study identifies BTN3A3 as a human restriction factor that inhibits avian influenza viruses but not human IAVs, with resistance linked to specific NP mutations.

CpG dinucleotide enrichment in the influenza A virus genome as a live attenuated vaccine development strategy

This paper engineers an influenza A virus with increased CpG content, showing it is attenuated in mice but replication competent in vaccine propagation systems, suggesting a new strategy for live attenuated vaccines.

Engineering a Vaccine Platform using Rotavirus A to Express SARS-CoV-2 Spike Epitopes

This paper tests engineering rotavirus to express SARS-CoV-2 spike epitopes, finding that inserting peptides into NSP3 is feasible while VP4 insertions impair viral replication.

Rapid selection of P323L in the SARS-CoV-2 polymerase (NSP12) in humans and non-human primate models and confers a large plaque phenotype

The paper tracks the rapid emergence of the P323L mutation in SARS-CoV-2 polymerase in human and non-human primate models, showing it confers a larger plaque phenotype compared to P323.

Secreted SARS-CoV-2 ORF8 modulates the cytokine expression profile of human macrophages

This study shows that SARS-CoV-2 ORF8 protein modulates cytokine expression in human macrophages, with sequence polymorphisms affecting IL-6 and IL-8 secretion.

STING Nuclear Partners Contribute to Innate Immune Signalling Responses

The paper identifies nuclear STING partners that contribute to innate immune signaling by interacting with IRF3/7 transcription factors and enhancing antiviral responses.

Contribution of segment 3 to the acquisition of virulence in contemporary H9N2 avian influenza viruses

This paper identifies a single amino acid change in the PA protein of H9N2 avian influenza viruses that enhances virulence and replication in poultry.

PA-X is an avian virulence factor in H9N2 avian influenza virus

This study investigates the role of PA-X in H9N2 avian influenza viruses, finding it enhances replication in mammalian cells and contributes to virulence in chickens.

Intra-genome variability in the dinucleotide composition of SARS-CoV-2

The paper analyzes CpG dinucleotide composition in SARS-CoV-2 and other coronaviruses to understand its role in replication and cross-species transmission.

Asparagine deprivation causes a reversible inhibition of Human Cytomegalovirus acute virus replication

This study identifies asparagine as a critical factor for HCMV replication through a large-scale siRNA screen, showing that its depletion inhibits virus replication but allows recovery with exogenous asparagine.

An alternative AUG codon in segment 5 of the 2009 pandemic influenza A virus is a swine-derived virulence motif

The paper identifies an alternative AUG codon in the 2009 pandemic influenza A virus as a virulence factor contributing to pathogenicity in swine and humans.

Constitutive TRIM22 expression within the respiratory tract identifies tissue-specific and cell-type dependent intrinsic immune barriers to influenza A virus infection

This study identifies TRIM22 as a constitutively expressed antiviral factor in the respiratory tract that restricts influenza A virus infection independently of cytokine-mediated immunity.

Comprehensive characterisation of molecular host-pathogen interactions in influenza A virus-infected human macrophages

This study characterizes molecular host-pathogen interactions in influenza A virus-infected human macrophages using CAGE sequencing, revealing snatching preferences and avoidance patterns of host RNAs.

Segment 2 from influenza A(H1N1)pdm09 viruses confers temperature sensitive HA yield on candidate vaccine virus growth in eggs that is complemented by PB2 701D

This study shows that pdm09 segment 2 in candidate vaccine viruses reduces HA yield in eggs at 37.5°C but this temperature sensitivity is compensated by the PB2 701D mutation, improving HA yield.

Mutation of influenza A virus PA-X decreases pathogenicity in chicken embryos and can increase the yield of reassortant candidate vaccine viruses

This paper shows that mutating the PA-X gene in influenza A virus reduces pathogenicity in chicken embryos and increases HA yield in vaccine reassortant viruses.

Pleotropic effects of mutations in the effector domain of influenza A virus NS1 protein

The paper investigates how mutations in the NS1 protein of influenza A virus affect its ability to promote viral mRNA nuclear export and interacts with cellular factors.