K. Campbell

GelBox: Open-source software to improve rigor and reproducibility when analyzing gels and immunoblots

GelBox improves rigor and reproducibility in gel and immunoblot analysis by integrating image adjustments, background correction, band-fitting, and metadata association in a single open-source tool.

Examining the molecular clock hypothesis for the contemporary evolution of the rabies virus

This paper investigates how variable incubation periods affect molecular clock models in rabies virus evolution and finds that per-generation models are equivalent to molecular clocks at realistic substitution rates.

Human light meromyosin mutations linked to skeletal myopathies disrupt the coiled coil structure and myosin head sequestration

This study investigates how mutations in MYH7 and MYH2 genes affect myosin structure and function, finding that they disrupt coiled-coil structure and increase ATP consumption without affecting contractile function.

Cryo-EM structure of the human cardiac myosin filament

This paper solves the cryo-EM structure of the human cardiac myosin filament, revealing the arrangement of myosin, cMyBP-C, and titin and their functional interactions.

Predominant myosin super-relaxed state in canine myocardium with naturally occurring dilated cardiomyopathy

This study finds that myosin in DCM-affected dogs has increased super-relaxed state and is rescued by EMD-57033.

Right Ventricular Sarcomere Contractile Depression and the Role of Thick Filament Activation in Human Heart Failure with Pulmonary Hypertension

This study identifies contractile deficits in right ventricular myocytes of HFrEF-PH patients, showing reduced calcium-activated tension and altered myosin ATP turnover, with implications for targeted therapies.

Spatial regulation of the glycocalyx component Podocalyxin is a switch for pro-metastatic function

This study identifies PODXL as a decoy receptor for GAL3, revealing its role in promoting metastasis through integrin-based invasion and differential cortical targeting regulated by ubiquitination.

Optimizing multifunctional fluorescent ligands for intracellular labeling

The paper designs cell-permeable multifunctional ligands for HaloTag fusions that enable protein labeling and translocation, using Si-rhodamines with low KL-Z and high logD7.4 values.

Cardiac myosin binding protein-C palmitoylation is associated with increased myofilament affinity, reduced myofilament Ca2+ sensitivity and is increased in ischaemic heart failure.

This study identifies palmitoylation of cMyBP-C in cardiac myofilaments, showing it reduces Ca2+ sensitivity and is altered in heart failure.

In vivo CRISPR screens reveal SCAF1 and USP15 as novel drivers of pancreatic cancer

This study uses in vivo CRISPR screens to identify USP15 and SCAF1 as novel tumor suppressors in pancreatic cancer, with potential therapeutic implications.

Cell-cell metabolite exchange creates a pro-survival metabolic environment that extends lifespan

The paper shows that metabolite exchange between cells extends lifespan in yeast by creating a pro-survival metabolic environment.

A multiscale model of the cardiovascular system that regulates arterial pressure via closed loop baroreflex control of chronotropism, cell-level contractility, and vascular tone

This paper extends a cardiovascular model to include baroreflex control of arterial pressure, demonstrating its ability to regulate pressure under various physiological challenges.

Shared and distinct genetic features in human and canine B-cell lymphomas

This study compares genetic mutations in canine and human B-cell lymphomas, identifying shared and distinct features that may affect their biology and therapeutic development.

Increased apoptotic priming of glioblastoma enables therapeutic targeting by BH3-mimetics

This study shows that glioblastoma (GBM) is highly sensitive to BH3-mimetics due to elevated anti-apoptotic BCL-xL and MCL-1 expression, which can be therapeutically targeted.

Sensitive high-throughput assays for tumour burden reveal the response of a Drosophila melanogaster model of colorectal cancer to standard chemotherapies

The paper develops high-throughput assays to measure tumour burden in a Drosophila CRC model and shows it responds to standard chemotherapies.

The yeast eIF2 kinase Gcn2 facilitates H2O2-mediated feedback inhibition of both protein synthesis and ER oxidative folding during recombinant protein production

This study shows that Gcn2 kinase mediates H2O2 feedback inhibition of protein synthesis and ER oxidative folding in yeast during recombinant protein production, and its removal enhances -amylase production.

Age-Related Delay in Visual and Auditory Evoked Responses is Mediated by White- and Gray-matter Differences

The paper investigates age-related differences in visual and auditory evoked responses using MEG and MRI, finding distinct structural correlates for each type of delay.