J. Sha

RAPMS 2.0 Improves Specificity and Throughput for Proteomic Identification of RNA Binding Proteins

RAP-MS 2.0 improves specificity and throughput for identifying RNA-binding proteins by reducing background and enabling reuse of lysate for multiple RNA captures.

Occurrence, risk assessment of antibiotics and antimicrobial resistance in Escherichia coli in typical rivers of Sichuan

This study assesses antibiotic and antimicrobial resistance in Escherichia coli from Sichuan rivers, finding high contamination and resistance rates, with specific antibiotics linked to ecological risk.

Biochemical characterization of extended-spectrum β-lactamases from Akkermansia genus

The paper characterizes β-lactamases from Akkermansia species, showing they exhibit extended-spectrum activity and lack transferable resistance genes.

Characterization and functional analysis of Toxoplasma Golgi-associated proteins identified by proximity labelling

This paper identifies and functionally analyzes novel Toxoplasma Golgi-associated proteins using proximity labeling and knockdown experiments, revealing their roles in the secretory pathway and potential as therapeutic targets.

A Bacteriophage Cocktail Targeting Yersinia pestis Provides Strong Post-Exposure Protection in a Rat Pneumonic Plague Model

This paper evaluates a bacteriophage cocktail, YPP-401, for post-exposure protection against pneumonic plague in rats, showing ~88% protection when administered intranasally or intraperitoneally 18 hours post-exposure.

ACD15, ACD21, and SLN regulate accumulation and mobility of MBD6 to silence genes and transposable elements.

The paper shows ACD15 and ACD21 are essential for MBD5/6 complex accumulation and mobility, which silences genes and transposable elements in Arabidopsis.

PEXEL is a proteolytic maturation site for both exported and non-exported Plasmodium proteins

The paper shows that non-exported Plasmodium proteins also contain PEXEL motifs that are processed by PMV, but specific residues in the mature N-terminus determine export.

The Toxoplasma gondii effector GRA83 modulates the host's innate immune response to regulate parasite infection

This paper identifies GRA83 as a Toxoplasma gondii effector that modulates host innate immunity, reducing parasite burden by enhancing inflammatory responses.

Toxoplasma gondii encodes an array of TBC-domain containing proteins including an essential regulator that targets Rab2 in the secretory pathway

This study identifies TgTBC9 as an essential TBC-domain protein in Toxoplasma gondii that regulates Rab2 and ER-to-Golgi trafficking, offering therapeutic targets for apicomplexan parasites.

Role of cytoneme-like structures and extracellular vesicles in Trichomonas vaginalis parasite: parasite communication

This study shows that Trichomonas vaginalis strains communicate via cytoneme-like structures and extracellular vesicles, affecting parasite adherence and potentially influencing clinical outcomes.

FAP106 is an interaction hub required for assembly of conserved and lineage-specific microtubule inner proteins at the cilium inner junction

This study identifies FAP106 as a critical interaction hub for assembling conserved and lineage-specific microtubule inner proteins at the cilium inner junction in Trypanosoma brucei.

A bacteriophage-based, highly efficacious, needle and adjuvant-free, mucosal COVID-19 vaccine

This paper develops a bacteriophage-based mucosal vaccine that induces strong immune responses and protects against SARS-CoV-2 and its variants without requiring needles or adjuvants.

A genetic model for in vivo proximity labeling of the mammalian secretome

This paper introduces a genetic mouse model for in vivo proximity labeling of the secretome, revealing organ-specific secretory profiles and serum trafficking.

Combinatorial viral vector-based and live-attenuated vaccines without an adjuvant to generate broader immune responses to effectively combat pneumonic plague

This study evaluates a combination of adenovirus vector and live-attenuated vaccines for pneumonic plague, showing that a two-dose regimen provides full protection against multiple challenges.

A novel Toxoplasma IMC sutures-associated protein regulates suture protein targeting and colocalizes with membrane trafficking machinery

This study identifies a novel Toxoplasma gondii protein that localizes to IMC sutures, regulates suture protein targeting, and colocalizes with membrane trafficking machinery.

A Universal Bacteriophage T4 Nanoparticle Platform to Design Multiplex SARS-CoV-2 Vaccine Candidates by CRISPR Engineering

This paper develops a CRISPR-engineered bacteriophage T4 nanoparticle platform to create multiplex SARS-CoV-2 vaccine candidates, showing potent immune responses and protection in animal models.

Adenovirus E1A Binding to DCAF10 Targets Degradation of AAA+ ATPases Required for Quaternary Assembly of Multiprotein Machines and Innate Immunity

The paper identifies that adenovirus E1A's C-terminal region interacts with host proteins to form a complex that degrades AAA+ ATPases, thereby inhibiting IRF3 stabilization and innate immune responses.

Ancient MAPK ERK7 is regulated by an unusual inhibitory scaffold required for Toxoplasma apical complex biogenesis

The paper identifies AC9 as an essential scaffold for ERK7 regulation during apical complex biogenesis in Toxoplasma gondii, revealing a novel inhibitory mechanism for an ancient MAPK.

Single-cell dissection of a rare human prostate basal cell carcinoma

This study characterizes the mutational and transcriptomic landscapes of a rare prostate basal cell carcinoma using single-cell sequencing, revealing unique gene expression and regulatory patterns.