T. Tian

Resident memory T cell development is associated with AP-1 transcription factor upregulation across anatomical niches.

This study identifies AP-1 transcription factors as key regulators of TRM development across tissues using scRNA-seq and ATAC-seq, revealing a novel mechanism for TRM reactivation.

An Unexpected Single-step Glycosylation Enables the Construction of Antibody-Biomacromolecule Conjugates as Therapeutics

This paper demonstrates a method to efficiently conjugate biomacromolecules to antibodies using modified Fc glycan and an engineered enzyme, enabling rapid and homogeneous antibody conjugates with therapeutic applications.

Chemoenzymatic Measurement of Cell-surface Glycan in Single-cell Multiomics: LacNAc as an Example

The paper introduces a chemoenzymatic method to detect cell-surface glycan in single-cell multiomics, showing increased LacNAc in activated CD8+ T cells and its correlation with glycolytic activity.

Sensitive bacterial Vm sensors revealed the excitability of bacterial Vm and its role in antibiotic tolerance

The paper develops bacterial Vm sensors to study Vm dynamics and shows that excitable states enhance antibiotic tolerance.

Flavivirus prM interacts with MDA5 and MAVS to inhibit RLR antiviral signaling

This study identifies how flavivirus prM proteins interact with MDA5 and MAVS to inhibit RLR antiviral signaling, revealing species-dependent immune evasion mechanisms.

Angiogenesis is critical for the regenerative effects of exercise.

This study shows that fibrin glue repair combined with exercise enhances axon regeneration and functional recovery by promoting angiogenesis in nerve repairs.

Ultrafast, one-step, and microwave heating-based synthesis of DNA/RNA-AuNP conjugates

This paper develops a microwave heating-based method to rapidly synthesize DNA/RNA-AuNP conjugates with high stability and broad applicability.

Membraneless organelles formed by liquid-liquid phase separation increase bacterial fitness

The paper identifies membraneless organelles in bacteria that enhance stress tolerance through liquid-liquid phase separation.

Characterization of endogenous Rubus yellow net virus in raspberries

This study characterizes endogenous Rubus yellow net virus (endoRYNV) in raspberries, revealing diverse endoRYNV sequences and their prevalence due to limited germplasm use in breeding programs.

Understanding the phase separation characteristics of nucleocapsid protein provides a new therapeutic opportunity against SARS-CoV-2

This study identifies that SARS-CoV-2 nucleocapsid protein undergoes phase separation, which can be modulated by small molecules, offering new therapeutic strategies against COVID-19.

A Community Challenge for Pancancer Drug Mechanism of Action Inference from Perturbational Profile Data

The paper hosts a DREAM Challenge to evaluate computational models for predicting drug polypharmacology using perturbational profile data from 32 kinase inhibitors.

Popularizing recombinant baculovirus-derived OneBac system for scaling-up production of all recombinant adeno-associated virus vector serotypes

This paper optimizes a baculovirus system to efficiently produce all rAAV serotypes 1-13, achieving high yields and scalability for research and therapeutic applications.

A DNA-origami nuclear pore mimic reveals nuclear entry mechanisms of HIV-1 capsid

This study uses DNA-origami NPC mimics to show HIV-1 capsid uses a trimeric interface and RRR motif to pass through nuclear pores, bypassing the FG motif of NUP153.

Skin Delivery of Modified Vaccinia Ankara Viral Vectors Generates Superior T Cell Immunity Against a Respiratory Viral Challenge

This study shows that skin scarification delivery of MVA vectors generates robust T cell immunity against respiratory viral challenges, outperforming other routes and suggesting its potential for vaccines like COVID-19.

Droplet-digital Cas13a assay enables direct single-molecule microRNA quantification

The paper develops a droplet-digital Cas13a assay for single-molecule microRNA quantification, demonstrating its precision, specificity, and broad applicability.

CASLFA: CRISPR/Cas9-mediated lateral flow nucleic acid assay

CASLFA enables rapid, specific detection of pathogens and GMOs with high sensitivity and no technical expertise.

Universal and naked-eye gene detection platform based on CRISPR/Cas12a/13a system

This paper develops a CRISPR-based colorimetric gene detection platform using AuNPs that enables naked-eye detection of DNA and RNA targets with high sensitivity and universality.

Escherichia coli’s physiology can turn membrane voltage dyes into actuators

The paper develops a workflow to characterize Nernstian membrane voltage dyes in E. coli, identifying conditions where dyes act as actuators rather than probes.

Genome-wide prediction of synthetic rescue mediators of resistance to targeted and immunotherapy

The paper predicts synthetic rescue mediators of resistance to targeted and immunotherapy using tumor transcriptomics and survival data from 10,000 TCGA patients, validating predictions through experimental screens.

Dynamic protein aggregation regulates bacterial dormancy depth critical for antibiotic tolerance

This study identifies dynamic protein aggregation as a regulator of bacterial dormancy depth, influencing antibiotic tolerance and resuscitation.