A. Johnson

A diverse proteome is present and enzymatically active in metabolite extracts

The paper shows that metabolite extracts contain active enzymes and demonstrates a new workflow to remove proteins for improved metabolomics.

Single-nanoparticle electrophoretic mobility determination and trapping using active-feedback 3D tracking

This paper develops a method to measure single-nanoparticle electrophoretic mobility and trap them using 3D tracking with active feedback, showing improved precision over ensemble methods.

NRF2-dependent regulation of the prostacyclin receptor PTGIR drives CD8 T cell exhaustion

This study identifies the KEAP1-NRF2 pathway as a driver of CD8 T cell exhaustion by regulating the prostacyclin receptor PTGIR.

Zinc-starved Brassicaceae Plants Secrete Peptides thatInduce Root Expansion

This study identifies secreted peptides in zinc-deficient Brassicaceae plants that promote root expansion to enhance zinc uptake.

Cooperativity of weak actomyosin interaction

The paper investigates the cooperative mechanism of weak actomyosin interaction in muscle, showing that myosin heads work together to produce force through cross-bridge clusters.

From birds to mammals: spillover of highly pathogenic avian influenza H5N1 virus to dairy cattle led to efficient intra- and interspecies transmission

This study reports the spillover of HPAI H5N1 virus to dairy cattle, showing efficient intra- and interspecies transmission and viral tropism in mammary gland epithelial cells.

Lipid availability influences ferroptosis sensitivity in cancer cells by regulating polyunsaturated fatty acid trafficking

The paper shows lipid-starved cancer cells are more sensitive to ferroptosis due to increased PUFA trafficking into phospholipids, challenging the assumption that PUFA levels directly determine ferroptosis susceptibility.

Antibody-mediated cellular responses are dysregulated in Multisystem Inflammatory Syndrome in Children (MIS-C)

This study identifies dysregulated antibody-mediated cellular responses in MIS-C, linking NK cell dysfunction to immune pathology and suggesting potential therapeutic interventions.

Super-resolution expansion microscopy in plant roots

This study develops and applies expansion microscopy to Arabidopsis root tissues, achieving improved resolution and visualizing subcellular structures.

Role of Dynamin-Related Proteins 2 and SH3P2 in Clathrin-Mediated Endocytosis in Plants

The paper investigates the role of DRP2s and SH3P2 in plant clathrin-mediated endocytosis, finding that DRP2s are recruited independently of BAR-SH3 proteins.

Acidification-dependent suppression of C. difficile by pathogenic and commensal enterococci

This study shows VRE suppresses C. difficile growth via acid production in vitro, but dietary fructose does not lower pH or prevent C. difficile colonization in VRE-colonized mice.

Active-feedback 3D single-molecule tracking using a fast-responding galvo scanning mirror

This paper introduces a fast-responding galvo scanning mirror for RT-3D-SPT, improving tracking performance for single molecules and nanoparticles.

Identifying the core genome of the nucleus-forming bacteriophage family and characterization of Erwinia phage RAY

The paper identifies a core genome of nucleus-forming bacteriophages and characterizes the replication mechanism of Erwinia phage RAY.

Habitat edges affect tree diversity more than biomass regeneration in a reforested wet neotropical timber plantation

This study examines how distance to intact forest edges affects tree diversity and biomass in a reforested wet neotropical plantation, finding that diversity is more impacted by edge effects than biomass.

Integrated PET and confocal imaging informs a functional timeline for the dynamic process of vascular reconnection during grafting.

This paper uses integrated PET and confocal imaging to establish a spatiotemporal timeline for vascular reconnection during grafting in tomato.

Lymphocyte networks are dynamic cellular communities in the immunoregulatory landscape of lung adenocarcinoma

The paper maps lymphocyte interactions in lung adenocarcinoma using spatial analysis and machine learning, revealing dynamic lymphocyte networks that support anti-tumor immune responses.

User-friendly electron microscopy protocols for the visualization of biological macromolecular complexes in three dimensions: Visualization of planta clathrin-coated vesicles at ultrastructural resolution

The paper presents user-friendly electron microscopy protocols for visualizing plant clathrin-coated vesicles in 3D at nanometer resolution, along with a deep learning workflow for analysis.

Sonic Hedgehog Activates Prostaglandin Signaling to Stabilize Primary Cilium Length

This study shows SHH signaling stabilizes primary cilium length through crosstalk with EP4 prostaglandin signaling, ensuring proper SHH pathway function.

The N-linker region of hERG1a upregulates hERG1b potassium channels

This study identifies a mechanism by which the hERG1a N-linker region enhances hERG1b potassium channel expression at the plasma membrane, potentially offering a therapeutic target for Long QT syndrome.

Brain endothelial antigen presentation detains CD8+ T cells at the blood-brain barrier leading to its breakdown

The paper shows that brain endothelial antigen presentation inhibits CD8+ T-cell movement and causes BBB breakdown in MS.

Proteomic Characterization of Isolated Arabidopsis Clathrin-Coated Vesicles Reveals Evolutionarily Conserved and Plant Specific Components

The paper characterizes the proteome of Arabidopsis clathrin-coated vesicles, identifying conserved and plant-specific components involved in clathrin-mediated trafficking.

The Genetic Architecture of Strawberry Yield and Fruit Quality Traits

This study identifies genetic factors influencing strawberry yield and quality traits, finding a trade-off between sugar content and yield and recommending trait selection strategies for breeding.

The TPLATE complex mediates membrane bending during plant clathrin-mediated endocytosis

The paper identifies the TPLATE complex as a mediator of membrane bending in plant clathrin-mediated endocytosis, which occurs independently of actin and against high turgor pressure.

Modulation of root growth by nutrient-defined fine-tuning of polar auxin transport

The paper investigates how nitrogen availability modulates root growth by fine-tuning polar auxin transport through post-translational modifications of PIN2 and presents a computational model to simulate these processes.

Development of mPing-based Activation Tags for Crop Insertional Mutagenesis

This paper develops mPing-based activation tags for crop insertional mutagenesis, showing they can induce gene overexpression and improve mutagenesis effectiveness.

Unique Evolution of Antiviral Tetherin in Bats

This study characterizes the evolution of tetherin in bats, identifying unique structural variants and splice isoforms with antiviral functions.

High temporal resolution reveals simultaneous plasma membrane recruitment of the TPLATE complex subunits.

The paper uses live cell imaging to show that the TPLATE complex is recruited to the plasma membrane as an octameric complex, not sequentially.

Emergence of a novel Salmonella enterica serotype Reading clone is linked to its expansion in commercial turkey production, resulting in unanticipated human illness in North America

This study identifies a novel Salmonella enterica serotype Reading clone linked to human outbreaks and commercial turkey production in North America through genomic analysis.

Movement patterns of urban free-roaming dogs: implications for rabies control in Arequipa, Peru

This study tracks free-roaming dogs in Arequipa to show how water channels influence their movement, aiding rabies spread beyond local control efforts.

Reproducible and Transparent Research Practices in Published Neurology Research

This study evaluates reproducibility in neurology research by analyzing access to materials, data, scripts, and preregistration in sampled publications.