This study shows that intranasal vaccination with SARS-CoV-2 S-RBD and mucosal adjuvant M7 enhances T central memory cells and cross-neutralizing antibodies against variants of concern compared to subcutaneous vaccination.

bioRxiv · Sep 9, 2022 · PreprintImmunology

E3 ligase TRIM47 positively regulates endothelial activation and pulmonary inflammation through potentiating the K63-linked ubiquitination

Y. Qian, Z. Wang, H. Lin, T. Lei, Z. Zhou, W. Huang, X. Wu, L. Zuo, J. Wu, Y. Liu, L.-F. Wang, X.-H. Guan, K.-Y. Deng, M. Fu, H.-B. Xin

This study shows TRIM47 enhances endothelial activation and pulmonary inflammation by promoting K63-linked ubiquitination of TRAF2, leading to NF-κB and MAPK pathway activation.

bioRxiv · Sep 15, 2021 · PreprintCell Biology

Rapid determination of the wide dynamic range of SARS-CoV-2 Spike T cell responses in whole blood of vaccinated and naturally infected

A. T. Tan, J. M. Lim, N. Le Bert, K. Kunasegaran, A. Chia, M. Qui, N. Tan, W. N. Chia, R. Alwis, Y. Ding, E. E. Ooi, L.-F. Wang, M. I. Chen, B. Young, L. Y. Hsu, , A. Bertoletti

This study evaluates a rapid whole blood test for measuring SARS-CoV-2 Spike-specific T cell responses in vaccinated and infected individuals, finding a wide dynamic range independent of neutralizing antibodies.

bioRxiv · Jun 29, 2021 · PreprintImmunology

A species-specific signature residue in the PB2 subunit of the bat influenza virus polymerase restricts viral RNA synthesis.

S. Banerjee, A. De, N. Kedia, L.-F. Wang, A. Mondal

bioRxiv · Apr 12, 2021 · PreprintMicrobiology

Highly functional virus-specific cellular immune response in asymptomatic SARS-CoV-2 infection

N. Le Bert, H. E. Clapham, A. T. Tan, W. N. Chia, C. Y. Tham, J. M. Lim, K. Kunasegaran, L. Tan, C.-A. Dutertre, N. Shankar, J. M. Lim, L. J. Sun, M. Zahari, Z. M. Tun, V. Kumar, , C. C. Tam

This study compares T cell responses in asymptomatic and symptomatic SARS-CoV-2感染者, finding that asymptomatic individuals have a robust and functional immune response with balanced cytokine production.

bioRxiv · Nov 25, 2020Immunology

Early induction of SARS-CoV-2 specific T cells associates with rapid viral clearance and mild disease in COVID-19 patients

A. T. Tan, M. Linster, C. Tan, N. Le Bert, W. Chia, K. Kunasegaran, Y. Zhuang, C. Y. Tham, A. Chia, G. J. Smith, B. E. Young, S. Kalimuddin, J. G. Low, D. Lye, L.-F. Wang, A. Bertoletti

The paper examines the relationship between SARS-CoV-2 specific T cell responses and disease severity in COVID-19 patients, finding that early T cell induction correlates with mild disease and faster viral clearance.

bioRxiv · Oct 15, 2020Immunology

Age and gender dependence of liver diffusion parameters and the evidence of intravoxel incoherent motion modelling of perfusion component is constrained by diffusion component.

H. Huang, C.-J. Zheng, L.-F. Wang, N. Che-Nordin, Y.-X. Wang

The paper establishes reference values for liver diffusion MRI parameters in middle-aged subjects and shows age and gender effects, with IVIM modeling indicating perfusion is constrained by diffusion.

bioRxiv · Aug 27, 2020Biophysics

Origin and cross-species transmission of bat coronaviruses in China

A. Latinne, B. Hu, K. J. Olival, G. Zhu, L. Zhang, H. Li, A. A. Chmura, H. E. Field, C. Zambrana-Torrelio, J. H. Epstein, B. Li, W. Zhang, L.-F. Wang, Z. Shi, P. Daszak

This study investigates the evolution, cross-species transmission, and origin of bat coronaviruses in China using sequence data and Bayesian analysis.

bioRxiv · May 31, 2020Evolutionary Biology

Different pattern of pre-existing SARS-COV-2 specific T cell immunity in SARS-recovered and uninfected individuals

N. Le Bert, A. T. Tan, K. Kunasegaran, C. Y. L. Tham, M. Hafezi, A. Chia, M. Chng, M. Lin, N. Tan, M. Linster, W. N. Chia, M. I.-C. Chen, L.-F. Wang, E. E. Ooi, S. Kalimuddin, , A. Bertoletti

The paper compares pre-existing SARS-CoV-2 specific T cell immunity in SARS-recovered and uninfected individuals, finding differential T cell immunodominance patterns.

bioRxiv · May 26, 2020Immunology

Unique Evolution of Antiviral Tetherin in Bats

J. A. Hayward, M. Tachedjian, A. Johnson, A. T. Irving, T. B. Gordon, J. Cui, A. Nicolas, I. Smith, V. Boyd, G. A. Marsh, M. L. Baker, L.-F. Wang, G. Tachedjian

This study characterizes the evolution of tetherin in bats, identifying unique structural variants and splice isoforms with antiviral functions.

bioRxiv · Apr 8, 2020Evolutionary Biology

Orthogonal genome-wide screenings in bat cells identify MTHFD1 as a target of broad antiviral therapy

D. E. Anderson, J. Cui, Q. Ye, B. Huang, W. Zu, J. Gong, W. Liu, S. Y. Kim, B. G. Yan, K. Sigmundsson, X. F. Lim, F. Ye, P. Niu, X. Zhou, W. Tan, , X. Tan

The paper identifies MTHFD1 as a target for broad antiviral therapy by screening bat cells with RNAi and CRISPR libraries against mumps and influenza viruses.

bioRxiv · Mar 29, 2020Microbiology

Discovery of a 382-nt deletion during the early evolution of SARS-CoV-2

Y. Su, D. Anderson, B. Young, F. Zhu, M. Linster, S. Kalimuddin, J. Low, Z. Yan, J. Jayakumar, L. Sun, G. Yan, I. Mendenhall, Y.-S. Leo, D. Lye, L.-F. Wang, G. Smith

The paper identifies a 382-nt deletion in SARS-CoV-2 ORF8 from Singapore patients, which enhances N gene transcription and may contribute to viral attenuation.

bioRxiv · Mar 11, 2020 · PreprintMicrobiology

Accelerated viral dynamics in bat cell lines, with implications for zoonotic emergence

C. E. Brook, M. Boots, K. Chandran, A. P. Dobson, C. Drosten, A. L. Graham, B. Grenfell, M. A. Müller, M. Ng, L.-F. Wang, A. van Leeuwen

The paper models viral dynamics in bat cell lines with constitutive immune responses, finding that robust antiviral defenses correlate with higher viral propagation rates and suggest implications for zoonotic emergence.

bioRxiv · Feb 1, 2020Ecology

Human MAIT cell cytolytic effector proteins synergize to overcome carbapenem resistance in Escherichia coli

C. Boulouis, W. R. Sia, M. Y. Gulam, J. Q. M. Teo, T. K. Phan, J. Y. W. Mak, D. P. Fairlie, I. K. H. Poon, T. H. Koh, P. Bergman, L.-F. Wang, A. L. H. Kwa, J. K. Sandberg, E. Leeansyah

This study shows human MAIT cells use granulysin and granzyme B to overcome carbapenem resistance in E. coli by increasing bacterial permeability and restoring carbapenem efficacy.

bioRxiv · Jan 16, 2020Immunology

Validation study of a diffusion MRI derived vessel density biomarker for detecting viral hepatitis-b induced liver fibrosis

Y.-X. Wang, B.-H. Xiao, L.-F. Wang, H. Huang, S.-W. Guo, S.-W. Qiu

This paper validates a diffusion MRI biomarker, DDVD/area, for detecting liver fibrosis in hepatitis-B patients, showing it effectively differentiates fibrotic from non-fibrotic livers.

bioRxiv · Jul 11, 2019 · PreprintBiophysics

Viral hijacking of the nucleolar DNA-damage response machinery: a novel mechanism to regulate host cell biology

S. M. Rawlinson, T. Zhao, A. M. Rozario, C. L. Rootes, P. J. McMillan, A. W. Purcell, A. Woon, G. A. Marsh, K. G. Lieu, L.-F. Wang, H. J. Netter, T. D. Bell, C. R. Stewart, G. W. Moseley

The paper identifies a novel viral mechanism that hijacks the nucleolar DNA-damage response pathway by interacting with Treacle to inhibit rRNA biogenesis.

bioRxiv · Nov 17, 2017Microbiology

The IFN response in bat cells consists of canonical and non-canonical ISGs with unique temporal expression kinetics

P. C. De La Cruz-Rivera, M. Kanchwala, H. Liang, A. Kumar, L.-F. Wang, C. Xing, J. Schoggins

This study identifies canonical and non-canonical ISGs in bat cells with distinct temporal expression kinetics, revealing unique aspects of the IFN response that may contribute to viral resistance.

bioRxiv · Jul 24, 2017Immunology