B. Qu

T cell polarization and NFAT translocation are stiffness-dependent and are differentially regulated by Piezo1 and Orai1

This study shows that T cell polarization and NFAT translocation are stiffness-dependent and are regulated by different Ca2+ influx pathways, Piezo1 and Orai1.

The transcription factor Zeb2 drives differentiation of age-associated B cells

The paper identifies Zeb2 as a critical transcription factor driving age-associated B-cell differentiation and autoimmunity in humans and mice.

High-throughput and in-depth analysis of plasma proteome by UV-assisted protein digestion, 5-plex labeling and MS-based high abundance protein removal method

This study develops a high-throughput plasma proteome analysis workflow using UV-assisted digestion, 5-plex labeling, and MS-based high abundance protein removal to improve quantification of low-abundance proteins.

Human pluripotent stem cell-derived hepatocyte-like cells for hepatitis D virus studies

This study shows that hepatocyte-like cells derived from human pluripotent stem cells are permissive to hepatitis D virus infection and can be used to study HDV biology and test therapies.

A high-throughput 3D kinetic killing assay

This paper introduces a high-throughput 3D kinetic killing assay to evaluate immune killer cell function and tumor cell viability under in vivo-like conditions.

PIEZO1-mediated mechanosensing governs NK cell killing efficiency and infiltration in three-dimensional matrices

This study shows that PIEZO1-mediated mechanosensing affects NK cell killing efficiency and infiltration in 3D matrices by responding to tumor cell stiffness.

Spatiotemporal analysis of SARS-CoV-2 infection reveals an expansive wave of monocyte-derived macrophages associated with vascular damage and virus clearance in hamster lungs

This study uses 3D spatiotemporal analysis to show that SARS-CoV-2 infection in hamsters leads to monocyte-derived macrophage infiltration, vascular damage, and virus clearance, followed by tissue remodeling and fibrosis.

In vitro evaluation of immune responses to bacterial hydrogels for the development of living therapeutic materials

This study evaluates immune responses to bacterial hydrogels, showing encapsulation reduces immunogenicity and apoptosis, making them suitable for living therapeutic materials.

SARS-CoV-2 entry route impacts a range of downstream viral and cellular processes

This study shows that SARS-CoV-2 entry via TMPRSS2 or endolysosomal cathepsins affects viral replication, immune response, and genome evolution.

The response mechanism of oreorchis patens (Lindl.) Lindl. to cold stress

This study investigates how Oreorchis patens responds to cold stress by analyzing physiological, biochemical, and molecular mechanisms, identifying differentially expressed genes and confirming their roles through qRT-PCR.

A noval lightsheet scattering microscopy for long-term visualization of cell-ECM interaction

This paper introduces a novel lightsheet scattering microscopy technique to visualize long-term cell-ECM interactions in 3D without photobleaching.

Bioactive constituents of Verbena officinalis alleviate inflammation and enhance killing efficiency of natural killer cells

This study shows that Verbena officinalis extract reduces inflammation and enhances NK cell killing efficiency, with Verbenalin specifically improving viral infection response.

High glucose enhances antigen-independent CTL killing via TRAIL

This study shows high glucose increases TRAIL expression in CTLs, promoting beta cell apoptosis, and identifies metformin and vitamin D as synergistic therapies to mitigate this effect.

Collagen density defines 3D migration of CTLs and their consequent cytotoxicity against tumor cells

This study shows that dense collagen matrices impair CTL migration and cytotoxicity, with microtubule networks playing a key role in restoring migration and function.

Profiling of cytokines, chemokines and growth factors in saliva and gingival crevicular fluid

This study compares cytokine, chemokine, and growth factor profiles in saliva and gingival crevicular fluid from healthy donors to determine which fluid is better for biomarker development.

T cell stiffness is enhanced upon formation of immunological synapse

This study investigates T cell stiffness changes during immunological synapse formation using atomic force microscopy, revealing differential stiffening in lamellipodia and cell body.

High glucose attenuates Ca2+ influx in cytotoxic T lymphocytes upon target recognition

This study shows high glucose increases store-operated calcium entry but reduces calcium influx during target recognition in CTLs, leading to increased necrosis after killing.

Optoregulated force application to cellular receptors using molecular motors

This paper introduces a light-driven molecular motor system to apply forces to cellular receptors, demonstrating its ability to manipulate cell adhesion and activation.

New Tricks for an old molecule: Preserved antibacterial activity of ribosomal protein S15 during evolution

The paper identifies RPS15 as an antimicrobial peptide with conserved antibacterial activity across species, showing it can kill bacteria through membrane disruption and ROS induction.

Migration of Cytotoxic T Lymphocytes in 3D Collagen Matrices

This study investigates the migration patterns of CD8+ T cells in 3D collagen matrices, identifying three motility types and proposing a two-state model to explain their movement through ECM.

HBV DNA is a substrate for the cGAS/STING pathway but is not sensed in infected hepatocytes

This study shows HBV DNA is sensed by cGAS/STING in hepatocytes but is not detected during infection due to capsid shielding, while HBV RNAs are not immunostimulatory.