A. Ashworth

De novo design of drug-binding proteins with predictable binding energy and specificity

This paper designs drug-binding proteins with low nanomolar affinity and predictable specificity using computational methods, validated by experimental measurements and structural analysis.

Targeting the Hippo pathway in cancers via ubiquitination dependent TEAD degradation

This study identifies RNF146 as an E3 ubiquitin ligase that degrades TEAD proteins via the proteasome, showing that pharmacological induction of TEAD ubiquitination can suppress YAP/TAZ-driven cancer growth.

NOTCH3 drives meningioma tumorigenesis and resistance to radiotherapy

This study identifies NOTCH3 as a driver of meningioma tumorigenesis and radiotherapy resistance, showing its role in tumor growth and survival in preclinical models.

A single inactivating amino acid change in the SARS-CoV-2 NSP3 Mac1 domain attenuates viral replication and pathogenesis in vivo

This study shows that mutating the SARS-CoV-2 NSP3 Mac1 domain reduces viral replication and pathogenesis in vivo.

Iterative computational design and crystallographic screening identifies potent inhibitors targeting the Nsp3 Macrodomain of SARS-CoV-2

The paper computationally designs and experimentally validates potent inhibitors of the SARS-CoV-2 Nsp3 Macrodomain, identifying 160 ligands and 152 crystal structures with submicromolar affinity.

Genome-wide CRISPR screens of T cell exhaustion identify chromatin remodeling factors that limit T cell persistence

This study identifies chromatin remodeling factors that limit T cell persistence using CRISPR screens and shows their impact on anti-tumor immunity.

The mechanisms of catalysis and ligand binding for the SARS-CoV-2 NSP3 macrodomain from neutron and X-ray diffraction at room temperature

This study determines the crystal structures of SARS-CoV-2 NSP3 macrodomain using neutron and X-ray diffraction, revealing insights into its catalytic mechanism and ligand binding.

Hypoxia is a dominant remodeler of the CD8+ T cell surface proteome relative to activation and regulatory T cell-mediated suppression

This study compares how hypoxia and regulatory T cells affect the CD8+ T cell surface proteome, finding hypoxia has a more significant impact than Treg-mediated suppression.

Targeting DNA damage response and Replication Stress in Pancreatic Cancer

The paper identifies novel therapeutic strategies for pancreatic cancer by analyzing DNA damage response and replication stress in patient-derived cell lines.

Detection of early stage pancreatic cancer using 5-hydroxymethylcytosine signatures in circulating cell free DNA

This study identifies 5-hydroxymethylcytosine (5hmC) signatures in circulating cell-free DNA to detect early-stage pancreatic cancer with high accuracy.

Genome-wide CRISPR Screens in Primary Human T Cells Reveal Key Regulators of Immune Function

This study uses CRISPR screens in primary human T cells to identify regulators of immune function and cancer cell killing.

Genome-wide and high-density CRISPR-Cas9 screens identify point mutations in PARP1 causing PARP inhibitor resistance

The paper identifies PARP1 point mutations causing PARP inhibitor resistance through CRISPR-Cas9 screens and shows their impact on PARP1 trapping and chemotherapy sensitivity.