E. Wolf

Identification of Suitable Target/E3 Ligase Pairs for PROTAC Development using a Rapamycin-induced Proximity Assay (RiPA)

This paper introduces a rapamycin-induced proximity assay (RiPA) to identify effective E3 ligase-target pairs for PROTAC development, demonstrating its ability to predict degradation capabilities.

Comparative phenotyping of mice reveals canonical and noncanonical physiological functions of TRα and TRβ

This study compares TRα and TRβ knockout mice with DNA-binding-deficient mutants to show noncanonical TR signaling contributes to physiological functions beyond canonical genomic pathways.

Gene Therapy for Cardiomyopathy associated with Duchenne Muscular Dystrophy in a Pig Model

This study shows that AAV1.SERCA2a gene therapy improves heart function and reduces arrhythmia in a pig model of Duchenne muscular dystrophy.

Increased pulmonary monocyte infiltration and attenuated phagocytosis defines perinatal dysfunction of innate immunity in Cystic Fibrosis

The paper identifies increased monocyte infiltration and reduced phagocytosis in CF lungs at birth, suggesting innate immune dysfunction and the need for early treatment.

Mucilage Produced by Sorghum 1 (Sorghum bicolor) Aerial Roots Hosts Diazotrophs that Provide Significant Amounts of Nitrogen to the Plant

This study identifies diazotrophs in sorghum aerial root mucilage that supply 40% of the plant's nitrogen, offering a sustainable alternative to synthetic fertilizers.

A structural competition involving WDR5 times circadian oscillations

This study identifies WDR5 as a direct interaction partner of PER1 and PER2, showing its role in modulating circadian oscillations through structural competition at the BMAL1/CLOCK interface.

A marine cryptochrome with an inverse photo-oligomerization mechanism

This study identifies a marine cryptochrome, PdLCry, that undergoes inverse photo-oligomerization compared to plant CRYs, enabling light intensity discrimination for lunar cycle synchronization.

Spt5 interacts genetically with Myc and is limiting for brain tumor growth in Drosophila

This study shows Spt5 interacts with Myc in Drosophila and is limiting for brain tumor growth, suggesting it as a potential drug target in human oncology.

New C3H KitN824K/WT cancer mouse model develops late-onset malignant mammary tumors with high penetrance

This paper describes a new mouse model with a KIT mutation that develops late-onset mammary tumors with high penetrance and lung metastasis.

Immature human engineered heart tissues engraft in a guinea pig chronic injury model

This study shows that transplanting immature engineered heart tissue patches improves cardiomyocyte engraftment in a chronic injury guinea pig model.

SbCYP79A61 Produces Phenylacetaldoxime, a Precursor of Benzyl Cyanide and Phenylacetic Acid in Sorghum bicolor

This study identifies SbCYP79A61 as an enzyme in sorghum that produces phenylacetaldoxime and shows benzyl cyanide as an intermediate in its biosynthesis pathway.

Multi-omics reveals principles of gene regulation and pervasive non-productive transcription in the human cytomegalovirus genome

The paper uses multi-omics data to model HCMV gene regulation, identifying over 2,600 viral transcripts and explaining protein expression kinetics through transcription start sites and RNA stability.

A scalable, clinically severe pig model for Duchenne muscular dystrophy

This paper develops a scalable pig model for Duchenne muscular dystrophy by breeding DMD carriers, enabling large cohorts for disease mechanism studies and therapeutic testing.

NOXA expression drives synthetic lethality to RUNX1 inhibition in pancreatic cancer

The paper identifies RUNX1 inhibition as a strategy to induce NOXA-dependent apoptosis in pancreatic cancer, showing it blocks tumor growth in vivo and in organoids.

Evolutionary footprints of a cold relic in a rapidly warming world

This paper studies the evolutionary history of the genus Cochlearia, revealing its adaptation to cold environments through polyploid evolution and metabolomics insights.

Sequential in vivo labeling of insulin secretory granule pools in INS-SNAP transgenic pigs

This study develops a transgenic pig model with SNAP-tagged insulin to investigate in vivo insulin secretory granule dynamics and turnover.

Design, Synthesis and Evaluation of WD-repeat containing protein 5 (WDR5) degraders

This study designs, synthesizes, and evaluates two series of WDR5 degraders, showing that linker length and nature affect degradation efficacy.

IM30 IDPs form a membrane protective carpet upon super-complex disassembly

This study identifies that IM30 forms a membrane-protecting carpet upon super-complex disassembly, revealing a novel mechanism of membrane stabilization by intrinsically disordered proteins.

Chronic hyperglycaemia drives functional impairment of lymphocytes in diabetic INSC94Y transgenic pigs

This study examines immune cell dysfunction in diabetic pigs, finding reduced T cell proliferation and altered metabolic activity linked to chronic hyperglycaemia.

Impact of porcine cytomegalovirus on long-term orthotopic cardiac xenotransplant survival

This study shows that porcine cytomegalovirus transmission reduces survival in pig heart xenotransplants and increases inflammatory markers and fibrinolytic dysfunction.

A central role of glutamine in Chlamydia infection

This study identifies glutamine as essential for Chlamydia's EB-RB transition and shows that host glutamine metabolism is reprogrammed to support bacterial growth.

Cellular and Molecular Probing of Intact Transparent Human Organs

The paper introduces SHANEL, a method to clear and label stiff human organs for 3D histology, revealing structural details in intact adult human brain, kidney, and pig pancreas.