B. Lee

Borrelia Burgdorferi binds Serum Amyloid A and Modulates Subcutaneous Adipose Tissue Immune Signaling

This study shows Borrelia burgdorferi binds serum amyloid A and modulates immune signaling in subcutaneous adipose tissue, identifying SAA as a host defense factor.

The HLA-E/NKG2A axis identifies a therapeutic vulnerability in BCG-unresponsive bladder cancer

This study identifies the HLA-E/NKG2A axis as a therapeutic target in BCG-unresponsive bladder cancer by showing its role in immune evasion and impaired effector cell function.

Hydrophilic/ Omniphobic droplet arrays for high-throughput and quantitative enzymology

This paper introduces DA-MEK, a high-throughput platform for enzyme variant screening and kinetic analysis using droplet arrays, demonstrating accurate and efficient measurement of catalytic efficiencies.

A temperature-sensitive and interferon-silent Sendai virus vector for CRISPR-Cas9 delivery and gene editing in primary human cells

This paper develops a temperature-sensitive and interferon-silent Sendai virus vector for CRISPR-Cas9 delivery, achieving high gene editing efficiency in human cells without triggering immune responses.

P66 is a bacterial mimic of CD47 that binds the anti-phagocytic receptor SIRPα and facilitates macrophage evasion by Borrelia burgdorferi

This study identifies P66, a bacterial protein mimicking CD47, which binds SIRPα to evade macrophage phagocytosis, and shows its role in Lyme Disease pathogenesis.

Tetracistronic Minigenomes Elucidate a Functional Promoter for Ghana Virus and Unveils Cedar Virus Replicase Promiscuity for all Henipaviruses

This paper develops tetracistronic minigenomes for henipaviruses to model their life cycle outside high biocontainment, revealing Cedar virus replicase promiscuity and functional GhV replicase with antiviral efficacy.

De novo antibody discovery in human blood from full-length single B cell transcriptomics and matching haplotyped-resolved germline assemblies

This study uses single-cell transcriptomics and germline haplotype assembly to discover antibodies from human blood post-MMR vaccination, showing neutralization of measles virus.

Deletion of Robo4 worsens neuroinflammation and motor coordination in a mouse model of Alzheimer's disease

This study shows that Robo4 deletion increases neuroinflammation and impairs motor coordination in Alzheimer's disease mice, with effects dependent on AD mutations and brain region.

Tumor-selective effects of active RAS inhibition in pancreatic ductal adenocarcinoma

This study evaluates the therapeutic potential of RMC-7977, a broad-spectrum RAS inhibitor, in pancreatic ductal adenocarcinoma models, showing strong anti-tumor effects with minimal impact on normal tissues.

High-throughput Microfluidic 3D Outer Blood-Retinal Barrier Model in a 96-Well Format: Analysis of Cellular Interactions and Barrier Function in Retinal Health and Disease

Decoding spatial organization maps and context-specific landscapes of breast cancer and its microenvironment via high-resolution spatial transcriptomic analysis

This study uses high-resolution spatial transcriptomics to analyze breast cancer and its microenvironment, revealing spatially distinct immune cell gradients and transcriptional differences between tumors.

Pan-cancer analysis reveals multifaceted roles of retrotransposon-fusion RNAs

The paper identifies cancer-specific transposon-fusion RNAs across 34 cancer types and finds they are associated with tumor suppressors and oncogenes, and can activate T-cells.

Understanding the heterogeneity of alloreactive natural killer cell function inkidney transplantation

This study identifies NK cell subpopulations and their functional responses in kidney transplant patients, linking pre-transplant NK cell characteristics to long-term graft function.

A marine bacterium elicits the type III secretion-dependent nonhost hypersensitive response in a land plant

A marine bacterium induces a nonhost hypersensitive response in a land plant through type III secretion systems, mediated by SGT1 and suppressed by AvrPto1.

An in vitro experimental pipeline to characterize the binding specificity of SARS-CoV-2 neutralizing antibodies

This paper characterizes the binding specificity and epitope of a broadly neutralizing anti-SARS-CoV-2 Spike RBD antibody using mAb-resistant virions and cryo-EM structural analysis.

Integrating EM and Patch-seq data: Synaptic connectivity and target specificity of predicted Sst transcriptomic types

The paper predicts MET-type identities from EM data and shows they have distinct connectivity patterns and synapse targets.

GITR and TIGIT immunotherapy provokes divergent multi-cellular responses in the tumor microenvironment of gastrointestinal cancers

The paper examines GITR and TIGIT immunotherapies in gastrointestinal cancers using ex vivo cultures and single-cell sequencing to identify their cellular mechanisms in the tumor microenvironment.

Crystal structure and solution state of the C-terminal head region of the narmovirus receptor binding protein

This paper determines the crystal structure and solution state of the C-terminal head region of the receptor-binding proteins from Mossman and Nariva viruses, revealing structural differences from other paramyxoviruses.

Proteogenomic Characterization Reveals Therapeutic Opportunities Related to Mitochondrial Function in Melanoma

This study uses proteomics to analyze mitochondrial function in melanoma samples, finding dysregulation linked to patient survival and treatment responses, and proposes therapeutic strategies targeting mitochondrial pathways.

TCOF1 is a single-component scaffold of the nucleolar fibrillar center

This study shows TCOF1 is a single-component scaffold for the nucleolar fibrillar center through homotypic interactions and sufficiency experiments.

A monoclonal antibody targeting the Nipah virus fusion glycoprotein apex imparts protection from disease

This study identifies a monoclonal antibody, mAb92, that targets the Nipah virus fusion glycoprotein and provides complete protection in hamsters.

Immunological landscape of human lymphoid explants during measles virus infection

The paper infects human lymphoid explants with measles virus and uses scRNA-Seq and flow cytometry to identify B cell preference and T cell resistance, along with ISG protein induction.

Host cell neddylation facilitates alphaherpesvirus entry in a virus-specific and cell-dependent manner

This study shows that host cell neddylation is critical for HSV-1 entry via endocytosis, while having little effect on entry of other alphaherpesviruses.

Microwell-Based Flow Culture Increases Viability and Restores Drug Response in Prostate Cancer Spheroids

This study introduces a Microwell Flow Device that improves spheroid viability and drug response in prostate cancer models by reducing hypoxia-related necrosis.

Adjuvant Discovery via a High Throughput Screen using Human Primary Mononuclear Cells

This paper describes a high-throughput screening method using human PBMCs to identify adjuvant candidates, resulting in a lead compound with in vivo adjuvanticity.

Transcriptome analysis of FOXO-dependent hypoxia gene expression identifies Hipk as a regulator of low oxygen tolerance in Drosophila

The paper identifies FOXO-dependent gene expression changes in Drosophila under hypoxia, revealing Hipk as a key regulator of hypoxia survival and uncovering FOXO's role in suppressing energy-intensive processes.

Distinct cell states define the developmental trajectories of mucinous appendiceal neoplasms towards pseudomyxoma metastases

This study uses single-cell RNA-sequencing to identify distinct cell states in appendiceal mucinous neoplasms and their progression to pseudomyxoma peritonei, revealing developmental trajectories and signaling pathway changes.

Enteric bacterial infection in Drosophila induces whole-body alterations in metabolic gene expression independently of the Immune Deficiency (Imd) signalling pathway.

This study shows that Drosophila infected with Pseudomonas entomophila exhibit increased metabolic gene expression and altered transcription factors, independent of the Imd pathway.

Elevated HLA-E and NKG2A as a consequence of chronic immune activation defines resistance to M. bovis BCG immunotherapy in Non-Muscle-Invasive Bladder Cancer

The paper identifies elevated HLA-E and NKG2A as markers of resistance to BCG immunotherapy in NMIBC, linked to immune escape mechanisms.

NKG2A and HLA-E define a novel alternative immune checkpoint axis in bladder cancer

This study identifies NKG2A and HLA-E as an alternative immune checkpoint axis in bladder cancer, showing NKG2A+ CD8 T cells can bypass HLA-ABC loss through TCR-independent cytotoxicity.