J. Luban

PRC1.6 localizes on chromatin with the human silencing hub (HUSH) complex for promoter-specific silencing

The paper identifies PRC1.6 as a partner of the HUSH complex in promoter-specific provirus silencing through chromatin colocalization and functional assays.

Cyclophilin A Facilitates HIV-1 DNA Integration

This study shows that Cyclophilin A promotes HIV-1 integration by enhancing preintegration complex activity, revealing a new role for CypA in the viral life cycle.

IFIH1 (MDA5) is required for innate immune detection of intron-containing RNA expressed from the HIV-1 provirus

This study identifies IFIH1 (MDA5) as essential for innate immune detection of intron-containing RNA from the HIV-1 provirus, linking it to chronic inflammation in HIV-1 patients.

Addressing the dNTP bottleneck restricting prime editing activity

This study identifies mutations to improve prime editing efficiency in low-dNTP environments by enhancing MMLV-reverse transcriptase and reducing SAMHD1 activity.

Protein design for evaluating vaccines against future viral variation

The paper designs SARS-CoV-2 Spike proteins with novel mutations to evaluate vaccine effectiveness against future viral variants using a deep learning model.

S:D614G and S:H655Y are gateway mutations that act epistatically to promote SARS-CoV-2 variant fitness

This study shows that S:D614G and S:H655Y mutations enhance SARS-CoV-2 infectivity by stabilizing the Spike protein in an open RBD conformation, acting epistatically with the polybasic S1/S2 cleavage site.

Regulation of Ebola GP conformation and membrane binding by the chemical environment of the late endosome

This study investigates how acidic pH, Ca2+, and anionic phospholipids in the late endosome regulate Ebola GP conformation and membrane binding, revealing key residues and mechanisms involved in virus entry.

The HIV-1 capsid core is an opportunistic nuclear import receptor

This study identifies HIV-1 capsid proteins as a nuclear import receptor that interacts with host nucleoporins and soluble factors to facilitate nuclear entry.

Conformational dynamics and allosteric modulation of the SARS-CoV-2 spike

This study investigates how ACE2 and antibodies affect the conformational dynamics of the SARS-CoV-2 spike protein using smFRET and FCS, revealing that D614G modulates RBD energetics similar to ACE2 binding.

Global profiling of human blood ILC subtypes reveals that NK cells produce homeostatic cytokine amphiregulin and sheds light on HIV-1 pathogenesis

This study identifies NK cell production of amphiregulin in human blood ILCs and links it to HIV-1 pathogenesis through cytokine signaling and immune cell dynamics.

Cytotoxic CD4 Development Requires CD4 Effectors to Concurrently Recognize Local Antigen and Encounter Infection-Induced IL-15

This study identifies factors driving CD4 T cells to differentiate into cytotoxic lung-resident ThCTL during influenza infection.

The DHODH Inhibitor PTC299 Arrests SARS-CoV-2 Replication and Suppresses Induction of Inflammatory Cytokines

This paper evaluates PTC299's ability to inhibit SARS-CoV-2 replication and reduce inflammatory cytokine production in tissue culture models.

Structural and Functional Analysis of the D614G SARS-CoV-2 Spike Protein Variant

This study shows the D614G SARS-CoV-2 spike protein variant is more infectious but has reduced ACE2 affinity and altered trimer conformation, affecting viral entry and antibody neutralization.

Single Cell RNA Profiling Reveals Adipocyte to Macrophage Signaling Sufficient to Enhance Thermogenesis

This study shows that adipocyte signals can drive iWAT browning through macrophage-like cells, independent of sympathetic nerves.

Reporter assays for Ebola virus nucleoprotein oligomerization, virion-like particle budding, and minigenome activity reveal the importance of nucleoprotein amino acid position 111

The paper develops reporter assays to study Ebola virus nucleoprotein (NP) function and finds that amino acid position 111 affects oligomerization, virion budding, and transcription/replication.

TRIM34 acts with TRIM5 to restrict HIV and SIV capsids

This study identifies TRIM34 as an HIV-1 capsid-targeting restriction factor that acts with TRIM5alpha to restrict HIV and SIV infection during reverse transcription.

Influence of different glycoproteins and of the virion core on SERINC5 antiviral activity

This study investigates how retroviral glycoproteins and cores influence SERINC5 antiviral activity, finding that sensitivity depends on both factors.

Cyclophilin A protects HIV-1 from restriction by human TRIM5α

This study shows that Cyclophilin A protects HIV-1 from restriction by human TRIM5α by shielding the viral capsid from early inhibition during infection.

TRIM5α restricts flavivirus replication by targeting the viral protease for proteasomal degradation.

This study shows TRIM5α restricts flavivirus replication by targeting the viral protease for proteasomal degradation, with implications for human susceptibility to emerging flaviviruses.

Constrained mutational sampling of amino acids in HIV-1 protease evolution

The paper investigates HIV-1 protease evolution by comparing experimental fitness landscapes with sequenced isolate data, showing mutational sampling influences multiple mutations.

HIV-1-induced cytokines deplete homeostatic ILCs and expand TCF7-dependent memory NK cells

This study shows HIV-1-induced cytokines deplete homeostatic ILCs and drive the expansion of TCF7-dependent memory NK cells.

Intron-containing RNA from the HIV-1 provirus activates type I interferon and inflammatory cytokines

The paper shows that HIV-1 provirus RNA activates innate immune responses through post-transcriptional elements, suggesting new therapeutic targets.