J. Shen

This paper introduces a method to discover rare rabbit monoclonal antibodies against small molecules using an indirect competitive screening approach, resulting in a highly stable antibody with exceptional halophilic properties.

This study shows that the P2Y2 receptor in monocytes increases tissue factor expression, contributing to endotoxemia death in mice, and suggests it as a potential drug target.

This study shows STEAP1 can support a cross-membrane electron transfer chain via FAD, and determines the structure of STEAP2 in complex with NADP+ and FAD.

This study shows that gasdermin signaling is essential for fracture healing, as its absence delays bone callus formation and reduces biomechanical properties.

This study shows that cyt b5 reductase, cyt b5, and SCD1 form a stable ternary complex mediated by transmembrane helices, enhancing electron transfer rates.

The paper identifies cell type-selective IL-13-responsive enhancers in airway epithelial cells and demonstrates their therapeutic potential for asthma by targeting mucin production.

This paper introduces impedance flow cytometry to rapidly assess viability of non-growing Corynebacterium glutamicum cultures producing glutamate.

The paper develops a framework to quantify DOM-microbe associations under global change, finding that molecular traits and nutrient enrichment influence these interactions differently in subarctic and subtropical climates.

The paper uses a genetically encoded pH biosensor to screen E. coli mutant libraries and identifies genes involved in pH homeostasis.

This study uses CpHMD simulations to analyze cysteine reactivity in kinase N-cap positions, finding that some are reactive while others are not, depending on nearby residues.

This study identifies DAB2IP as a tumor suppressor in colon cancer that inhibits p53 degradation by competing with GRP75 for binding, thereby enhancing p53 stability and suppressing tumor growth.

This study calculates fentanyl's dissociation time from the mu-opioid receptor using molecular dynamics simulations and shows how protonation states affect binding and unbinding mechanisms.

This study shows sustained oxygenation improves diabetic wound healing by enhancing epithelialization, angiogenesis, and reducing inflammation without drugs.

This study shows YAP1 promotes breast cancer metastasis by enhancing TIAM1 expression through its enhancer, leading to increased RAC1 activity and invadopodia formation.

This study shows IL-13 reduces SARS-CoV-2 infection in bronchial epithelial cells by altering gene expression and inhibiting viral replication.

This study identifies SNHG1 as a regulator of LPS-induced acute lung injury by modulating the miR-421/TIMP3 axis in A549 cells.

Under constant infection by Sclerotinia sclerotiorum, BnaA03.WRKY28 interacts with BnaA09.VQ12 and takes precedence over phosphorylated BnWRKY33 to bind to the BnWRKY33 promoter, thereby weakening resistance but promoting branching.

This paper develops a robotic platform with tactile sensing and machine learning to digitize and classify 3D human pulse patterns, achieving 98.7% and 84.2% classification rates for three TCM pulse types.

The paper develops a method to validate gene function in sorghum by improving transformation efficiency and creating a pathway for high-throughput molecular genotyping.

The paper shows that bone marrow adipocyte whitening during obesity drives the increase of invasive Ly6Chigh monocytes, preceding adipose tissue macrophage expansion.

This study presents the structure of primate ferroportin and elucidates its mechanism of iron transport and regulation by hepcidin.

The paper reports cryo-EM structures of SARS-CoV-2 RdRp with nucleoside inhibitors, revealing their mechanism and guiding the design of more potent derivatives.

This study identifies deoxyartemisinin and artemisinic acid from Artemisia annua as active against Mycobacterium smegmatis, with artemisinic acid showing the highest efficacy.

This study uses CpHMD simulations to investigate the pH-dependent structure-dynamics-function relationship of human renin, revealing the roles of the catalytic dyad and flap conformation.

This study investigates the conformational dynamics of yeast mitochondrial transcription initiation using single-molecule techniques, revealing dynamic transitions and backtracking behavior.

This study elucidates the binding mechanism of fentanyl to the mu-opioid receptor using simulation techniques, revealing a novel secondary binding mode involving a conserved histidine.

This study identifies the ERK2-p53 complex as a therapeutic target in KRAS-mutant stomach/colorectal cancers, showing that disrupting it with MEK1/2 and ERK2 inhibitors induces apoptosis.

This study evaluates YM-254890's functional selectivity as a G protein inhibitor in human coronary artery endothelial cells, finding it inhibits both Gq and Gs proteins but not Gi/o.

This paper introduces a continuous constant pH molecular dynamics method to simulate proton-coupled conformational dynamics of transmembrane proteins.

This study shows JQ-1 reduces liver fibrosis in Schistosoma-infected mice by inhibiting JAK2/STAT3 signaling in hepatic stellate cells.