J. Shen

Single-cell profiling reveals the intratumor heterogeneity and immunosuppressive microenvironment in cervical adenocarcinoma

This study uses single-cell RNA sequencing to identify unique cell clusters and immunosuppressive features in cervical adenocarcinoma compared to squamous cell carcinoma.

c-di-GMP modulates ribosome assembly by inhibiting rRNA methylation

This study shows c-di-GMP inhibits rRNA methylation by binding to RlmI, modulates ribosome assembly, and promotes antibiotic tolerance in E. coli.

An anti-SARS-CoV-2 formula consisting volatile oil from TCM through inhibiting multiple targets in cultured cells

This paper develops a volatile oil formula from traditional Chinese medicine that inhibits SARS-CoV-2 by targeting multiple viral and host proteins in cultured cells.

Repression of PRMT activities sensitize homologous recombination-proficient ovarian and breast cancer cells to PARP inhibitor treatment

This study identifies PRMT inhibitors as effective combinational therapies with PARP inhibitors to sensitize HR-proficient ovarian and breast cancer cells to treatment.

The under-recognized dominance of magnetosome gene cluster-containing bacteria in oxygen-stratified freshwater ecosystems

The paper identifies a high abundance of magnetosome gene cluster-containing bacteria in oxygen-stratified freshwater environments, revealing Deltaproteobacteria as dominant rather than Magnetococcia.

Multi-omic Analysis Identifies Glioblastoma Dependency on H3K9me3 Methyltransferase Activity

This study identifies H3K9me3 methyltransferases as key drivers in glioblastoma, showing their association with prognosis, immune response, and drug response through multi-omics analysis.

Cdc13 exhibits dynamic DNA strand exchange in the presence of telomeric DNA

This study shows that Cdc13 can dynamically exchange DNA strands in the presence of telomeric DNA, revealing a mechanism for its movement at telomeres.

β-glucan induced trained immunity enhances antibody levels in a vaccination model in mice

This study shows that β-glucan-induced trained immunity in mice enhances antibody levels after vaccination by improving antigen presentation and T-cell responses.

HNF&alpha is a target of the Wnt/beta-catenin pathway and regulates colorectal carcinogenesis

This study shows HNF4 is upregulated in colorectal cancer and promotes tumorigenesis by being activated by the Wnt/β-catenin pathway.

Novel non-immunogenic trained immunity inducing small molecule with improved anti-tumor properties

The paper identifies a small molecule, A1155463, that induces trained immunity in macrophages without being immunogenic, enhancing anti-tumor resistance in mice.

High-fidelity enhanced AsCas12a knock-in mice for efficient multiplexed gene editing, disease modeling and orthogonal immunogenetics

Why is the Omicron main protease of SARS-CoV-2 less stable than its wild-type counterpart? A crystallographic, biophysical, and theoretical study of the free enzyme and its complex with inhibitor 13b-K

This study investigates why the Omicron variant's main protease is less stable than the wild-type by analyzing its structure, enzymatic activity, and biophysical properties.

YAP promotes chromosomal instability by transcriptionally activating AJUBA through its super enhancer in breast cancer

This study identifies YAP's role in promoting chromosomal instability in breast cancer by activating AJUBA through its super enhancer, leading to AURKA activation and aberrant mitosis.

QM/MM simulations of EFGR with afatinib reveal the role of the beta-dimethylaminomethyl substitution

The paper uses QM/MM simulations to study the reaction mechanism of afatinib with Cys797 in EGFR, revealing a Michael addition mechanism involving deprotonation and stabilization by Asp800.

The EGF/EGFR-YAP1/TEAD2 axis-mediated BRAF inhibitor vemurafenib resistance upregulates STIM1 in melanoma

This study identifies the EGF/EGFR-YAP1/TEAD2 axis as a mechanism by which vemurafenib resistance upregulates STIM1 in melanoma.

Screening for metastasis-related genes in mouse melanoma cells through sequential tail vein injection

This study screens for metastasis-related genes in mouse melanoma cells using sequential tail vein injection and identifies differentially expressed genes associated with metastasis.

Image-Based Assessment of Natural Killer Cell Activity against Glioblastoma Stem Cells

This study develops two image-based assays to assess NK cell migration and cytotoxicity against GSCs, aiming to improve NK cell-based immunotherapy for GBM.

Mechanism of Dimer Selectivity and Binding Cooperativity of BRAF inhibitors

This study uses molecular dynamics simulations to uncover the mechanism of dimer selectivity and binding cooperativity of BRAF inhibitors, revealing structural changes and hydrogen bonding that influence inhibitor binding preferences.

Senescent cells impair fracture repair through elevating ubiquitin-proteasome system activity in aged mice.

This study shows senescent cells impair fracture repair in aged mice by increasing ubiquitination of PDGFRβ via TGFβ, suggesting a new therapeutic target.

Incorporation of collagen into Pseudomonas aeruginosa, Staphylococcus aureus, and Burkholderia pseudomallei biofilms enhances their elasticity and resistance against phagocytic clearance

This study shows that incorporating collagen into biofilms of Pseudomonas aeruginosa, Staphylococcus aureus, and Burkholderia pseudomallei increases their elasticity and resistance to phagocytosis.

A spatially mapped gene expression signature for intestinal stem-like cells identifies high-risk precursors of gastric cancer

The paper identifies a 26-gene expression signature in high-risk gastric intestinal metaplasia (GIM) that localizes to intestinal stem-like cells and is associated with increased gastric cancer risk.

Identification of an osteopontin-derived peptide that binds neuropilin-1 and activates vascular cells

This paper identifies FOL-026, an osteopontin-derived peptide that binds neuropilin-1 and activates vascular cells, mimicking VEGF effects and showing potential for vascular repair.

Rapid and visual detection of Schistosoma japonicum circulating nucleic acids by CRISPR-Cas12a

This paper develops a CRISPR-Cas12a-based method for detecting Schistosoma japonicum circulating nucleic acids with high sensitivity and specificity, validated using rabbit serum samples.

Intrinsically disordered CsoS2 acts as a general molecular thread for α-carboxysome shell assembly

The paper investigates the role of intrinsically disordered CsoS2 in α-carboxysome shell assembly using synthetic engineering and cryoEM structures, revealing its function as a molecular thread and identifying a conserved interaction motif.

Alternative splicing of a potato disease resistance gene maintains homeostasis between development and immunity

The paper shows that the potato resistance gene RB uses alternative splicing to balance growth and immunity, with the pathogen effector IPI-O1 promoting splicing to enhance immunity.

A conserved and druggable pocket in class B G protein coupled receptors for orally active small molecule agonists

This study identifies a conserved binding pocket in class B GPCRs that enables small molecule agonists like PCO371 to activate G proteins without triggering arrestin signaling.

Molecular architecture and conservation of an immature human endogenous retrovirus

The paper determines the structure of immature HERV-K VLPs using cryoET STA, revealing conserved assembly mechanisms across retroviruses.

Single-chain fluorescent integrators for mapping G-protein-coupled receptor agonists

The paper introduces SPOTIT and SPOTall sensors to detect GPCR agonist localization in the whole brain using fluorescent integrator sensors for multiple neuromodulators.

Mechanism of the Temperature-Dependent Self-Assembly and Polymorphism of Chitin

This study uses molecular dynamics to show how chitin self-assembles into different structures at varying temperatures, revealing the role of hydrogen bonds and hydrophobic effects in its polymorphism.

Carotenoid assembly regulates quinone diffusion and the Roseiflexus castenholzii reaction center-light harvesting complex architecture

This study investigates how carotenoid assembly affects quinone diffusion and the structure of the reaction center-light harvesting complex in Roseiflexus castenholzii.