M. Zhou

Optimization of gene knockout approaches and practical solutions to sgRNA selection challenges in hPSCs with inducible Cas9 system

This study optimizes gene knockout in hPSCs using an inducible Cas9 system and identifies effective sgRNAs by comparing experimental results with cleavage efficiency algorithms.

Native mass spectrometry of membrane protein-lipid interactions in different detergent environments

This study uses native mass spectrometry to investigate how different detergents and charge-reducing molecules affect protein-lipid interactions in membrane proteins.

The Hippo pathway effector YAP inhibits NF-kB signaling and ccRCC growth by opposing ZHX2

This study shows YAP inhibits NF-κB signaling and ccRCC growth by blocking ZHX2, a co-factor of NF-κB, and competes with ZHX2 for binding to p65.

GLARE: Discovering Hidden Patterns in Spaceflight Transcriptome Using Representation Learning

GLARE applies representation learning to spaceflight transcriptomics, confirming existing findings and uncovering new gene expression patterns in Arabidopsis under spaceflight conditions.

Fungal pathogens exposed - genomic and phenotypic insights into Candida auris and its relatives of the Candida haemulonii species complex

This study analyzes genomes and phenotypes of Candida auris and related species to identify pathogenic traits and virulence factors.

Tradeoffs in ATP metabolisms via hypoxic gradient migration assays

The paper develops a microfluidic assay to study ATP metabolism tradeoffs in HaCaT cells under hypoxia and hyperglycemia, revealing a metabolic switch between stationary and motile modes.

Molecular Basis for RNA Cytidine Acetylation by NAT10

This study determines the cryo-EM structure of NAT10 bound to a cytidine-CoA probe, revealing its mechanism for RNA N4-cytidine acetylation and its role in cellular processes.

Spatial top-down proteomics for the functional characterization of human kidney

This paper develops a spatial proteomics workflow to identify and map proteoforms in human kidney tissue, revealing spatially distinct proteomic profiles between glomeruli and tubules.

Leveraging inter-individual transcriptional correlation structure to infer discrete signaling mechanisms across metabolic tissues

The paper identifies inter-individual transcriptional correlations across metabolic tissues to infer signaling mechanisms, validating findings with known biological roles.

Multiplexed gene editing with a multi-intron containing Cas9 gene in citrus

This paper introduces a multiplex gene editing system in citrus using a multi-intron Cas9 gene and gRNA-tRNA arrays to target the PDS gene, improving editing efficiency in Carrizo citrange and Duncan grapefruit.

VviWRKY10 and VviWRKY30 co-regulate powdery mildew resistance through modulating SA and ET-based defenses in grapevine

This study shows VviWRKY10 and VviWRKY30 regulate powdery mildew resistance in grapevine by modulating SA and ET defenses, with mutual transcriptional inhibition between the two genes.

A 3-component module maintains sepal flatness in Arabidopsis

The paper identifies a 3-component module involving VIP4, ARF3, and VANGUARD1 that maintains sepal flatness in Arabidopsis by regulating cell wall stiffness and growth balance.

An automated archival single-nucleus total RNA sequencing platform mapping integrative and retrospective cell atlas of gliomas

This paper introduces an automated single-nucleus RNA sequencing platform for analyzing archival glioma samples, revealing molecular characteristics and recurrence-related targets.

TRIM72 restricts lyssavirus infection by inducing K48-linked ubiquitination and proteasome degradation of the matrix protein

This study identifies TRIM72 as a host factor that restricts lyssavirus infection by promoting ubiquitination and proteasomal degradation of the viral matrix protein.

Kinetochore and ionomic adaptation to whole genome duplication

This study identifies genetic adaptations to whole genome duplication in Cochlearia species, highlighting kinetochore and ion transport gene changes across multiple WGD events.

Cryo-EM structures of human SID-1 transmembrane family proteins and implications for their low-pH-dependent RNA transport activity

The paper presents cryo-EM structures of human SIDT1 and SIDT2, revealing their dimeric architecture and demonstrating low-pH-dependent RNA binding and oligomerization.

The evolution of the duckweed ionome mirrors losses in structural complexity

This study examines the evolution of the duckweed ionome across species with varying structural complexity, finding decreasing calcium and magnesium levels and increased cadmium accumulation in rootless species.

Temporal dynamics and genomic programming of plasma cell fates

This study identifies a novel PC transition state and shows that long-lived plasma cells arise from late-phase GC responses through TIGIT-dependent clonal expansion.

Extracellular filaments revealed by affinity capture cryo-electron tomography of lymphocytes

This study demonstrates an affinity capture method for preparing T-cells and Jurkat cells for cryo-ET, revealing extracellular filamentous structures and improving workflow efficiency.

XRN1 deletion induces PKR-dependent cell lethality in interferon-activated cancer cells

The paper shows that deleting XRN1 activates PKR and kills cancer cells with high interferon expression, suggesting XRN1 as a therapeutic target.

Butyrate Protects against SARS-CoV-2-induced Tissue Damage in Golden Hamsters.

This study shows butyrate protects against SARS-CoV-2-induced tissue damage in golden hamsters by enhancing antiviral immunity and reducing oxidative stress.

Comprehensive catalogs for microbial genes and metagenome-assembled genomes of the swine lower respiratory tract microbiome identify the relationship of microbial species with lung lesions

The paper constructs gene catalogs and metagenome-assembled genomes from the swine lower respiratory tract microbiome and identifies associations between microbial species and lung lesions.

Replication stress inducing ELF3 upregulation promotes BRCA1-deficient breast tumorigenesis in luminal progenitors

The paper identifies replication stress as a driver of BRCA1-deficient breast tumorigenesis in luminal progenitors, showing ELF3 upregulation promotes this process.

Dissecting the molecular basis for the modulation of neurotransmitter GPCR signaling by GINIP

This study identifies the molecular mechanism by which GINIP modulates GPCR signaling through conformational changes in its PHD domain, essential for Gi subunit binding and regulation of G-protein signaling.

The central nervous system is a potential reservoir and possible origin of drug resistance in HBV

This study finds HBV can persist in cerebrospinal fluid, showing distinct mutations and resistance patterns compared to serum, and HIV co-infection increases resistance mutations in both compartments.

Free ferrous ions sustain activity of mammalian stearoyl-CoA desaturase-1

This study shows free ferrous ions sustain SCD1 activity by replenishing the diiron center during catalysis, revealing dynamic structural changes and a regulatory role for labile Fe2+ in lipid metabolism.

Dominant Role of Stochastic Processes in Soil Fungal Communities in Pioneer Forests at a Regional Scale

This study shows that stochastic processes dominate soil fungal community assembly in Populus davidiana forests, with elevation having a greater impact than seasonality.

Lipoprotein sorting to the cell surface via a crosstalk between the Lpt and Lol pathways during outer membrane biogenesis

The paper identifies lipoproteins transported to the cell surface via LptDE and reveals a crosstalk between Lpt and Lol pathways in Gram-negative bacteria.

Lineage-mosaic and mutation-patched spike proteins for broad-spectrum COVID-19 vaccine

This study develops a bivalent vaccine using lineage-mosaic and mutation-patched spike proteins that elicit broad-spectrum antibodies and protect against multiple SARS-CoV-2 variants in hamsters.

Caught in the Act: targeted mutagenesis of the herpesvirus fusogen central helix captures transition states

This study mutates the herpesvirus fusogen gB to capture its transition state, revealing the critical role of domain III in membrane fusion.