W. Tan

Molecular Architecture of Monkeypox Mature Virus

This study uses cryo-ET and STA to reveal the architecture of mature MPXV particles and identifies irregular virions with distorted core walls.

A Single-Short Partial Reprogramming of the Endothelial Cells decreases Blood Pressure via attenuation of EndMT in Hypertensive Mice

This study shows that reprogramming endothelial cells in hypertensive mice with OSK factors reduces blood pressure by inhibiting EndMT and vascular damage.

Novel antibody language model accelerates IgG screening and design for broad-spectrum antiviral therapy

This paper introduces an antibody language model (AbLM) to accelerate IgG screening and design for broad-spectrum antiviral therapy, achieving higher precision in predicting variant-resistant antibodies.

Blocker-SELEX: A Structure-guided Strategy for Developing Inhibitory Aptamers Disrupting Undruggable Transcription Factor Interactions

This paper introduces Blocker-SELEX, a structure-guided method to design inhibitory aptamers that disrupt transcription factor interactions, demonstrating efficacy against SCAF4/SCAF8-RNAP2 and WDR5-MYC complexes.

Perturbations of glutathione and sphingosine metabolites in Port Wine Birthmark patient-derived induced pluripotent stem cells

This study identifies metabolic perturbations in PWB-derived iPSCs, showing upregulated glutathione and downregulated sphingolipid metabolism, which may contribute to laser resistance in PWB vasculature.

circDesign Algorithm for Designing Synthetic Circular RNA

This paper introduces circDesign, an algorithm for designing synthetic circular RNA that improves stability and protein translation efficiency, demonstrated using rabies virus glycoprotein.

Supporting materials: Endothelial cells differentiated from patient dermal fibroblast-derived induced pluripotent stem cells resemble vascular malformations of Port Wine Birthmark

This study generates iPSCs from PWB fibroblasts and differentiates them into ECs that recapitulate disease phenotypes, identifying dysregulated pathways and genes involved in vascular malformations.

The SARS-CoV-2 Spike protein induces long-term transcriptional perturbations of mitochondrial metabolic genes, causes cardiac fibrosis, and reduces myocardial contractile in obese mice

This study shows that the SARS-CoV-2 Spike protein causes long-term mitochondrial gene suppression, cardiac fibrosis, and reduced myocardial contractility in obese mice.

Molecular evolution of protein sequences and codon usage in monkeypox viruses

This study investigates the molecular evolution of protein sequences and codon usage in monkeypox viruses, identifying positive selection, accelerated evolution, and codon deoptimization patterns.

Dissecting the contributions of tumor heterogeneity on metastasis at single-cell resolution

This study analyzes single-cell transcriptomes of primary tumors and metastases from breast cancer models, identifying transcriptional differences and gene signatures linked to metastatic potential and patient outcomes.

The Spike protein of SARS-CoV-2 impairs lipid metabolism and increases susceptibility to lipotoxicity: implication for a role of Nrf2

This study shows that the SARS-CoV-2 Spike protein disrupts lipid metabolism and autophagy, increasing lipotoxicity via ferroptosis, which can be mitigated by Nrf2 inhibition.

Nicotinamide Riboside alleviates exercise intolerance in ANT1-deficient mice.

This study shows that nicotinamide riboside improves exercise capacity in ANT1-deficient mice by increasing NAD+ levels and mitochondrial respiration.

Inhibition of SAR S-CoV-2 infection and replication by lactoferrin, MUC1 and α-lactalbumin identified in human breastmilk

This study identifies lactoferrin, MUC1, and α-lactalbumin in human breastmilk as antiviral components that inhibit SARS-CoV-2 infection and replication, including variants.

The novel conserved NAD+ binding micropeptide SGHRT regulates mitochondrial function and metabolism in human cardiomyocytes

Development of a Novel PROTAC using the Nucleic Acid Aptamer as a Targeting Ligand for Tumor Selective Degradation of Nucleolin

This paper develops a novel PROTAC, ZL216, using an aptamer as a targeting ligand to selectively degrade nucleolin in breast cancer cells, inhibiting their proliferation and migration.

Rescuable effect of R-salbutamol in LPS-induced immune dysfunction of sepsis

This study investigates the preventive and therapeutic effects of R-salbutamol, S-salbutamol, and their racemic mixture in a mouse model of LPS-induced sepsis, finding R-salbutamol improves survival and lung function while S-salbutamol worsens outcomes.

A tandem-repeat dimeric RBD protein-based COVID-19 vaccine ZF2001 protects mice and nonhuman primates

ZF2001, a tandem-repeat dimeric RBD protein vaccine, induces strong immune responses and protects mice and non-human primates from SARS-CoV-2 infection.

Tumor protein D52 (TPD52) affects cancer cell metabolism by negatively regulating AMPK.

This study shows TPD52 inhibits AMPK activity, leading to metabolic defects in mice, highlighting its role in cancer metabolism.

Thiophosphopeptides Instantly Targeting Golgi Apparatus and Selectively Killing Cancer Cells

The paper introduces thiophosphopeptides that target the Golgi apparatus and selectively kill cancer cells through enzymatic self-assembly.

Neutralization of SARS-CoV-2 VOC 501Y.V2 by human antisera elicited by both inactivated BBIBP-CorV and recombinant dimeric RBD ZF2001 vaccines

This study evaluates the neutralization of SARS-CoV-2 501Y.V2 variant by antisera from two Chinese vaccines, showing preserved neutralizing titres against the variant.

Ferritin nanoparticle based SARS-CoV-2 RBD vaccine induces persistent antibody response and long-term memory in mice

This study shows a ferritin nanoparticle-based SARS-CoV-2 RBD vaccine induces persistent antibodies and long-term memory in mice for at least 7 months.

Spike Protein of SARS-CoV-2 Activates Macrophages and Contributes to Induction of Acute Lung Inflammations in Mice

This study shows that SARS-CoV-2 Spike protein activates macrophages and induces acute lung inflammation in mice.

Characterization and structural basis of a lethal mouse-adapted SARS-CoV-2

A mouse-adapted SARS-CoV-2 strain with RBD mutations caused 100% mortality in aged male BALB/c mice and showed altered receptor binding affinity.

Evaluation of azithromycin or hydroxychloroquine plus azithromycin combination therapy on cardiac conduction and function in guinea pigs

This study evaluates the cardiac effects of azithromycin and hydroxychloroquine plus azithromycin in guinea pigs, finding QT interval prolongation and cardiac dysfunction with both treatments.

A comparison of Remdesivir versus Au22Glutathione18 in COVID-19 golden hamsters: a better therapeutic outcome of gold compound

This study compares the therapeutic effects of Remdesivir and Au22Glutathione18 in COVID-19 golden hamsters, finding that the gold compound better suppresses viral replication and inflammation.

CDK-Mediated Phosphorylation of FANCD2 Promotes Mitotic Fidelity

This study identifies CDK-mediated phosphorylation of FANCD2 at S592 as a regulator of its monoubiquitination during S-phase, which is critical for mitotic fidelity.

SARS-CoV-2 Quasispecies provides insight into its genetic dynamics during infection

The paper analyzes SARS-CoV-2 quasispecies structure using SMRT sequencing, revealing a One-King model with dominant and minor haplotypes and their evolutionary dynamics.

Comparative transcriptome analysis reveals the intensive early-stage responses of host cells to SARS-CoV-2 infection

This study compares the early host responses to SARS-CoV-2, SARS-CoV, and MERS-CoV infections using transcriptome analysis, revealing distinct patterns of cytokine and antiviral gene expression.

Scutellaria baicalensis extract and baicalein inhibit replication of SARS-CoV-2 and its 3C-like protease in vitro

This study shows that Scutellaria baicalensis extract and its compound baicalein inhibit SARS-CoV-2 replication and 3CLpro activity in vitro.

Orthogonal genome-wide screenings in bat cells identify MTHFD1 as a target of broad antiviral therapy

The paper identifies MTHFD1 as a target for broad antiviral therapy by screening bat cells with RNAi and CRISPR libraries against mumps and influenza viruses.