M. Singer

A Broad-Spectrum Multi-Antigen mRNA/LNP-Based Pan-Coronavirus Vaccine Induced Potent Cross-Protective Immunity Against Infection and Disease Caused by Highly Pathogenic and Heavily Spike-Mutated SARS-CoV-2 Variants of Concern in the Syrian Hamster Model

This paper develops a multi-antigen mRNA/LNP-based pan-CoV vaccine that provides cross-protective immunity against multiple SARS-CoV-2 variants in a hamster model.

A non-canonical Arp2/3 complex is essential for Plasmodium DNA segregation and transmission of malaria.

This study identifies a non-canonical Arp2/3 complex in Plasmodium essential for DNA segregation during male gametogenesis and transmission to mosquitoes.

Therapeutic Prime/Pull Vaccination of HSV-2 Infected Guinea Pigs with the Ribonucleotide Reductase 2 (RR2) Protein and CXCL11 Chemokine Boosts Antiviral Local Tissue-Resident and Effector Memory CD4+ and CD8+ T Cells and Protects Against Recurrent Genital Herpes

This study shows that a prime/pull vaccine using HSV-2 RR2 protein and CXCL11 chemokine boosts CD4+ and CD8+ T cells in DRG and VM tissues, reducing recurrent genital herpes severity and frequency.

Anti-Viral and Anti-Inflammatory Therapeutic Effect of RAGE-Ig Protein Against Multiple SARS-CoV-2 Variants of Concern Demonstrated in K18-hACE2 Mouse and Syrian Golden Hamster Models

This study shows RAGE-Ig reduces viral replication and inflammation in multiple SARS-CoV-2 variants using animal models and human cells.

High Frequencies of Antiviral Effector Memory TEM Cells and Memory B Cells Mobilized into Herpes Infected Vaginal Mucosa Associated With Protection Against Genital Herpes

This study shows that CCL28 mobilizes HSV-specific memory B and T cells in vaginal mucosa, providing protection against genital herpes.

A Multi-Epitope/CXCL11 Prime/Pull Coronavirus Mucosal Vaccine Boosts the Frequency and the Function of Lung-Resident CD4+ and CD8+ Memory T Cells and Protects Against COVID-19-like Symptoms and Death Caused by SARS-CoV-2 infection

Leveraging IFNγ/CD38 regulation to unmask and target leukemia stem cells in acute myelogenous leukemia

This paper develops a T cell engager that uses IFNγ to upregulate CD38 on leukemia stem cells, enabling their targeting and depletion in AML.

A central protein complex essential for Invasionin Toxoplasma gondii

The paper identifies a central protein complex in Toxoplasma gondii essential for invasion, showing that disruption of this complex blocks rhoptry secretion and host cell invasion.

Gram-positive bacteria secrete RNA aptamers to activate human STING for IL-1β release.

This study identifies RNA aptamers secreted by Gram-positive bacteria that activate the STING pathway to induce IL-1β release in human macrophages.

Circulating Clonally Expanded T Cells Reflect Functions of Tumor Infiltrating T Cells

The paper examines circulating T cells sharing TCRs with tumor-infiltrating T cells in metastatic melanoma patients, finding they exhibit effector gene signatures similar to tumor T cells.

Fluorescent tagging of Plasmodium circumsporozoite protein allows imaging of sporozoite formation but blocks egress from oocysts

The paper uses fluorescent tagging of CSP to study sporozoite formation and egress, finding some GFP-CSP fusions block egress from oocysts.

Single-cell analyses identify circulating anti-tumor CD8 T cells and markers for their enrichment

The paper identifies circulating anti-tumor CD8 T cells using single-cell RNA sequencing and TCR sequencing, and develops marker panels for their detection in mice and melanoma patients.

CglB adhesins secreted at bacterial focal adhesions mediate gliding motility

The paper identifies CglB as a substratum-coupling protein secreted by the gliding complex in Myxococcus xanthus, revealing a mechanism for force transduction and a novel secretion process.

Impaired Layer Specific Retinal Vascular Reactivity Among Diabetic Subjects

This study examines layer-specific retinal vascular reactivity in diabetic patients and finds impaired responses to oxygen and carbon dioxide in deep retinal layers.

Colonizations cause host shifts, diversification of preferences and expansion of butterfly diet breadth

The paper shows that colonizations drive host shifts and temporary diet breadth expansions in butterflies, while persistent populations become more specialized, explaining latitudinal patterns of diet breadth.

Modulation of bacterial multicellularity via spatiotemporal polysaccharide secretion

The paper shows that Myxococcus xanthus uses temporally and spatially controlled polysaccharide secretion to coordinate multicellular behaviors like swarm migration and biofilm formation.

An endogenous glucocorticoid-cytokine signaling circuit promotes CD8+ T cell dysfunction in the tumor microenvironment

This study identifies a glucocorticoid-cytokine signaling circuit in the tumor microenvironment that promotes CD8+ T cell dysfunction and correlates with poor response to checkpoint blockade in melanoma.

Liver-Chip: Reproducing Human and Cross-Species Toxicities

The paper designs Liver-Chips for rat, dog, and human to replicate species-specific liver toxicities and predict human relevance.

Use of IFNγ/IL10 ratio for stratification of hydrocortisone therapy in patients with septic shock

This paper identifies the IFNγ/IL10 ratio as a biomarker to guide hydrocortisone therapy in septic shock, showing improved survival when treatment aligns with the ratio's prediction.

Formin-2 drives intracellular polymerisation of actin filaments enabling correct segregation of apicoplasts in Plasmodium falciparum and Toxoplasma gondii.

This study shows Formin-2 is essential for F-actin nucleation and apicoplast segregation in apicomplexan parasites.

Cationic charge and polyspecificity of an integrin domain regulates infectivity of malaria parasites

This study shows that the I-domain of TRAP in Plasmodium parasites mediates adhesion and that its cationic charge and polyspecificity are critical for infectivity.

The Effects of Practice-based Training on Graduate Teaching Assistants’ Classroom Practices

This study evaluates a practice-based training program for graduate teaching assistants, finding that frequent technique use was observed but participation-enforcing techniques were not consistently adopted.