W. Shen

Generation of a biliary tract cancer cell line atlas reveals molecular subtypes and therapeutic targets

This study creates a biliary tract cancer cell line atlas using multi-omics data to identify molecular subtypes and therapeutic targets.

Mutating the arginine residue within the FRNK motif of HC-Pro yield highly attenuated strains that confer complete cross protection against telosma mosaic virus (TelMV) in passion fruit (Passiflora edulis)

A rapid and efficient in vivo inoculation method for introducing tree stem canker pathogens onto leaves: Suitable for large-scale assessment of resistance in poplar breeding progeny

This paper introduces an in vivo leaf inoculation method for assessing poplar resistance to V. sordida, showing it is more efficient and suitable for large-scale screening than traditional stem segment inoculation.

An Engineered IGF2 Mutant for Lysosomal Targeting Chimeras Development and Membrane Proteins Degradation

This paper introduces eiLYTACs, an engineered IGF2-based technology that degrades membrane proteins and inhibits tumor growth by avoiding IGF1R activation.

Identification and characterization of a temperature sensitive chlorotic soybean mutant

The paper identifies a temperature-sensitive chlorotic soybean mutant and shows that a mutation in Glyma.07G102300 causes the phenotype, with severity increasing at lower temperatures.

Virus-induced gene silencing (VIGS) as a tool for functional genetic analysis in passion fruit plants

This paper develops a VIGS vector in passion fruit to study gene function in biosynthesis of health-promoting compounds.

Thiolation-Based Protein-Protein Hydrogels for Improved Wound Healing

This paper develops protein-protein hydrogels using thiolation and Ag-S bonding to enhance wound healing properties in both diabetic and UV-burn models.

A redundant chloroplast protein is co-opted by potyvirids as the scaffold protein to mediate viral intercellular movement complex assembly

This study identifies HCPro2 as a potyvirus-encoded scaffold protein that interacts with viral and host factors to assemble an intercellular movement complex, promoting viral infection.

Bio-inspired mineralization collagen induce fibrocartilage regeneration after tendon-bone injury by activating Gli1+Dkk3+ progenitor cells

This study develops a bio-inspired hydrogel that promotes tendon-bone interface regeneration by activating Gli1+Dkk3+ progenitor cells, achieving 68% mechanical recovery.

POLARIS is a copper-binding peptide required for ethylene signalling control in Arabidopsis.

The paper identifies PLS as a copper-binding peptide that modulates ethylene signaling in Arabidopsis by interacting with ETR1 and regulating copper-dependent repression of ethylene responses.

Cotyledon Peeling Method for Passion Fruit Protoplasts: a versatile cell system for transient gene expression in passion fruit (Passiflora edulis)

This paper introduces a new method for isolating and transforming passion fruit protoplasts, achieving high yield and transfection efficiency for transient gene expression.

Multi-omics of the gut microbial ecosystem in the immunotherapy resistance in microsatellite instability-high gastrointestinal cancer patients

The paper uses multi-omics data to identify gut microbiome and metabolite markers of immunotherapy resistance in MSI-H/dMMR gastrointestinal cancer patients and develops a predictive model with 0.83 accuracy.

Changes of platelet morphology, ultrastructure and function in patients with acute ischemic stroke based on super-resolution microscopy.

This study uses super-resolution microscopy to analyze platelet morphology, ultrastructure, and function in acute ischemic stroke patients, revealing structural and functional changes and demonstrating the utility of the technique for assessing antiplatelet drugs.

MERISTEM-DEFECTIVE/DEFECTIVELY ORGANIZED TRIBUTARIES2 regulates the balance between stemness and differentiation in the root meristem through RNA splicing control.

This study identifies MDF as a splicing factor regulating root meristem function in Arabidopsis by controlling splicing of RSZ33, ACC1, and SR34, balancing stemness and stress response.

A bi-functional PARP-HDAC inhibitor with activity in Ewing sarcoma

This paper develops a dual PARP and HDAC inhibitor, kt-3283, showing enhanced cytotoxicity and efficacy in Ewing sarcoma models compared to single-agent inhibitors.

Crystal structure of monkeypox H1 phosphatase, an Antiviral Drug Target

The paper determines the crystal structure of monkeypox H1 phosphatase, revealing its dimeric form and active site, which informs antiviral drug development.

Copresent microbiome and short-chain fatty acids profiles of plant biomass utilization in rumen simulation technique system

This study investigates microbiome and short-chain fatty acids (SCFAs) profiles in rumen simulation technique (RUSITEC) systems inoculated with goat and cow rumen contents, identifying copresent microbial genomes associated with substrate degradation.

Evolution of nasal and olfactory infection characteristics of SARS-CoV-2 variants

This study examines how SARS-CoV-2 variants infect nasal and olfactory tissues, showing differences in tropism, immune response, and regeneration across age groups.

Maternal Western-Style Diet Impairs Bone Marrow Development and Drives a Hyperinflammatory Phenotype in Hematopoietic Stem and Progenitor Cells in Fetal Rhesus Macaques

This study shows that a maternal Western-style diet impairs fetal bone marrow development and causes hyperinflammation and B-cell dysfunction in fetal hematopoietic stem and progenitor cells in rhesus macaques.

Liver X Receptor; Controls Hepatic Stellate Cell Activation via Hedgehog Signaling

This study shows that LXRβ suppresses hepatic stellate cell activation via inhibition of Hedgehog signaling, offering a potential therapeutic target for liver fibrosis without causing liver steatosis.

Super-dosed butyrate induces a revisable metabolic paralysis through transient mitochondrial reprogramming in the gut-brain axis

This study shows that high-dose sodium butyrate causes reversible metabolic paralysis by inducing transient mitochondrial reprogramming in the brain, specifically through ANT protein activation and proton leak.

MiRNA-223 Inhibits MouseGallstone Formation by Targeting Key Transporters in Hepatobiliary Cholesterol Secretion Pathway

This study shows that miRNA-223 inhibits gallstone formation in mice by targeting ABCG5 and ABCG8, key transporters in the bile cholesterol secretion pathway.

Discovery and Development of Human SARS-CoV-2 Neutralizing Antibodies using an Unbiased Phage Display Library Approach

This study identifies and develops human SARS-CoV-2 neutralizing antibodies using a phage display library, showing their efficacy in vivo against the virus.

Inhibition of mitochondrial respiration impairs nutrient consumption and metabolite transport in human retinal pigment epithelium

This study shows that inhibiting mitochondrial respiration in RPE cells alters intracellular and extracellular metabolites, revealing a metabolic signature of mitochondrial dysfunction.

Characterization and repurposing of the endogenous Type I-F CRISPR-Cas system of Zymomonas mobilis for genome engineering

This study characterizes and repurposes the endogenous Type I-F CRISPR-Cas system of Zymomonas mobilis to create an efficient genome engineering toolkit with 100% efficiency in various genetic modifications.