X. Shi

Mitochondrial choline import regulates purine nucleotide pools via SLC25A48

This study identifies SLC25A48 as a mitochondrial carrier that mediates choline import, regulating purine nucleotide synthesis and cell survival.

Protocatechuic acid induces endogenous oxidative stress in CR-hvKP by regulating the EMP-PPP pathway

This study shows that protocatechuic acid (PCA) enhances antibiotic efficacy against carbapenem-resistant hypervirulent Klebsiella pneumoniae by inducing oxidative stress through regulation of the EMP-PPP pathway.

The assembly and efflux processes revealed by in situ structures of the ABC-type tripartite pump MacAB-TolC

The paper resolves the in situ structures of the MacAB-TolC efflux pump in E. coli, revealing its assembly process and substrate-driven efflux mechanism.

UBA1-CDK16: A Sex-Specific Chimeric RNA and Its Role in Immune Sexual Dimorphism

The paper identifies a female-specific chimeric RNA, UBA1-CDK16, and shows its role in myeloid differentiation and immune sexual dimorphism.

An Efficient and Safe Trans-complementation System for MPXV Mimicking Authentic Viral Infection

This paper develops a safe trans-complementation system for MPXV that mimics authentic infection, enabling research in lower biosafety labs without live virus.

Structural and mechanistic views of enzymatic, heme-dependent nitrogen-nitrogen bond formation

This study investigates the structural and mechanistic basis of N-N bond formation by the heme-dependent enzyme PipS, revealing divergent pathways and a shared nitrenoid intermediate.

Proximity Labeling Expansion Microscopy (PL-ExM) resolves structure of the interactome

PL-ExM combines proximity labeling and expansion microscopy to visualize 3D interactome structures and assess PL technique performance.

Dual regulation of SLC25A39 by AFG3L2 and iron controls mitochondrial glutathione homeostasis

The paper identifies dual regulation of SLC25A39 by AFG3L2 and iron, showing how this controls mitochondrial glutathione homeostasis in neurons.

Lac-Phe mediates the anti-obesity effect of metformin

This study identifies Lac-Phe as a mediator of metformin's anti-obesity effects through experiments in mice and human cohorts.

The SAM Domain of EphA2 Inhibits Ligand-Independent Clustering and Activation

This study investigates the role of the SAM domain in EphA2, showing it inhibits ligand-independent clustering and kinase activity in both full-length and intracellular domain constructs.

Depleting glioblastoma cells of very long-chain acyl-CoA synthetase 3 (ACSVL3) produces metabolic alterations in non-lipid pathways

This study shows that knocking out ACSVL3 in glioblastoma cells reduces their malignant traits by altering non-lipid metabolic pathways, including glucose and energy metabolism.

The tRNA Gm18 Methyltransferase TARBP1 Promotes Hepatocellular Carcinoma Progression via Metabolic Reprogramming of Glutamine

This study identifies TARBP1 as a driver gene in hepatocellular carcinoma that promotes tumor progression by methylating tRNA and enhancing glutamine metabolism.

High-resolution structure of mouse radial spoke and its in-situ structure in ependymal cilia revealed by cryo-EM and cryo-ET

The paper presents high-resolution cryo-EM structures of mouse radial spokes and cryo-ET maps of ependymal cilia, revealing structural features and mutations linked to ciliary disorders.

Synergistic effects of multiple pesticides on the flight ability of honey bees (Apis cerana F.)

This study shows that combinations of imidacloprid, chlorpyrifos, and glyphosate synergistically reduce flight ability in honey bees, while individual pesticides do not.

VERY LONG-CHAIN ACYL-CoA SYNTHETASE-3 (ACSVL3) PROMOTES THE MALIGNANT GROWTH BEHAVIOR OF U87 GLIOMA CELLS VIA CHANGES IN CELL CYCLE WITHOUT AFFECTING APOPTOSIS

This study shows that reducing ACSVL3 expression in U87 glioma cells slows their growth and tumor formation in mice by altering the cell cycle without affecting apoptosis.

ZBP1-mediated macrophage necroptosis inhibits ASFV replication

This study identifies ZBP1-mediated macrophage necroptosis as a mechanism that inhibits ASFV replication by promoting proinflammatory cytokine release.

Hepatic conversion of acetyl-CoA to acetate plays crucial roles in energy stresses

The paper identifies acetate as a novel energy stress marker produced in the liver via ACOT12/8-mediated conversion of acetyl-CoA from fatty acids, supporting its role in brain fueling and CoA recycling.

CYP4F2 is a human-specific determinant of circulating N-acyl amino acid levels

The paper identifies CYP4F2 as a human-specific genetic determinant of plasma N-acyl amino acid levels and shows its role in metabolic diversification.

A simple, flexible and high-efficiency western blot analysis for age-related human induced neurons

This paper develops a high-efficiency western blot method using PTSA to analyze age-related human induced neurons and identifies a decline in Parkinson's disease-associated proteins.

TERT accelerates BRAF mutant-induced thyroid cancer dedifferentiation and progression by regulating ribosome biogenesis

This study shows TERT promotes thyroid cancer dedifferentiation and progression by enhancing ribosome biogenesis, and inhibiting rRNA synthesis can reverse this process.

Large polyacrylamide hydrogels for large-batch cell culture and mechanobiological studies

This paper introduces a method to prepare large-batch polyacrylamide hydrogels with tunable stiffness for mechanobiological studies and cell culture.

In situ structures of secretins from bacterial type II secretion system reveal their membrane interactions and translocation process

The paper determines in situ structures of GspD and GspDβ secretins during translocation, revealing distinct membrane interaction patterns and translocation mechanisms.

Soft multimodal opto-electric biointerfaces for co-localized optical and electrical recording of cell function

This paper introduces a flexible opto-electric device that co-locally records optical and electrical signals from cells with minimal crosstalk and high performance.

Characterization of a novel botybirnavirus with a unique dsRNA infecting Didymella theifolia from tea plants

The paper characterizes a novel botybirnavirus, DtBRV1, found in Didymella theifolia, which infects tea plants, and identifies a unique dsRNA unit with distinct molecular features.

Extracellular vimentin is sufficient to promote cell attachment, spreading, and motility by a mechanism involving N-acetyl glucosamine-containing structures

This study shows extracellular vimentin promotes cell attachment, spreading, and motility via N-acetyl glucosamine-containing structures, identifying a novel adhesion mechanism.

Transparent and stretchable metal nanowire composite recording microelectrode arrays

This paper develops stretchable, transparent microelectrode arrays using gold-coated silver nanowires, demonstrating high optical transparency, low impedance, and stable performance for biomedical applications.

SPRAY-INDUCED GENE SILENCING IDENTIFIES PATHOGEN PROCESSES CONTRIBUTING TO POWDERY MILDEW PROLIFERATION

This study uses spray-induced gene silencing to identify powdery mildew genes and processes critical for proliferation, showing conserved targets across species.

Droplet microfluidics forward for tracing target cells at single-cell transcriptome resolution

This paper develops a droplet-based method for single-cell transcriptome profiling of TCR-T cells, improving recovery and reducing contamination.

Orientation-locked DNA origami for stable trapping of small proteins in the NEOtrap

This paper improves the NEOtrap by orienting DNA origami to enhance trapping of small proteins, achieving longer observation times and reduced noise.

PERK Kinase Activity is Regulated by Copper Binding-A New Regulatory Paradigm for Modulation of ER Stress Tolerance

The paper shows copper binding regulates PERK kinase activity, affecting ER stress tolerance in C. elegans.