F. Deng

Efficient Gene Editing and Overexpression of Gametophyte Transformation in a Model Fern

This paper develops a CRISPR/Cas9 system for efficient gene editing and overexpression in the fern Ceratopteris richardii, demonstrating functional effects on stomatal and photosynthetic traits.

Establishing Primary and Stable Cell Lines from Frozen Wing Biopsies for Cellular, Physiological, and Genetic Studies in Bats

This paper develops a protocol for isolating and culturing primary fibroblasts from frozen bat wing biopsies and establishes stable cell lines for genetic and physiological studies.

Crypt and Villus Enterochromaffin Cells are Distinct Stress Sensors in the Gut

This study identifies distinct roles for crypt and villus enterochromaffin cells in sensing gut irritants and oxidative stress through different TRP channels and signaling mechanisms.

Exomap1 mouse: a transgenic model for in vivo studies of exosome biology

The exomap1 mouse model expresses HsCD81mNG to study exosome biology in vivo, showing cell type-specific exosome contributions and confirming CD81 as an exosome-specific marker.

Molecular Evolution and Interaction of 14-3-3 Proteins with H+-ATPases in Plant Abiotic Stresses

This paper reviews the roles of 14-3-3 proteins and H+-ATPases in plant abiotic stress responses and analyzes their molecular evolution across species.

Osseointegration of functionally-graded Ti6Al4V porous implants: Histology of the pore network

This study compares osseointegration of functionally-graded and uniform porous Ti6Al4V implants in rabbit tibiae, finding that smaller pores promote better bone volume and implant contact.

Real-time denoising of fluorescence time-lapse imaging enables high-sensitivity observations of biological dynamics beyond the shot-noise limit

This paper introduces DeepCAD-RT, a real-time denoising method for fluorescence time-lapse imaging that improves signal-to-noise ratio with fewer photons, demonstrated across multiple organisms and biological processes.

A serotonergic axon-cilium synapse drives nuclear signaling to maintain chromatin accessibility

The paper identifies axo-ciliary synapses that mediate serotonin signaling to regulate chromatin accessibility in hippocampal neurons.

Occurrence of COVID-19 symptoms during SARS-CoV-2 infection defines waning of humoral immunity

The study compares antibody responses in asymptomatic and symptomatic SARS-CoV-2感染者, finding that symptomatic individuals maintain higher and longer-lasting IgG and neutralizing capacities.

Low-disturbance Farming Regenerates Healthy Deep Soil towards Sustainable Agriculture

This study shows that low-disturbance farming practices improve deep soil health, microbial diversity, and crop yield over ten years, supporting sustainable agriculture.

Reporting quality of trend analyses published in leading medicine and oncology journals during 2008-2018

This study assesses the reporting quality of trend analyses in leading medicine and oncology journals from 2008-2018, finding moderate quality with room for improvement.

Discovery of a novel coronavirus associated with the recent pneumonia outbreak in humans and its potential bat origin

This paper identifies and characterizes a novel coronavirus (nCoV-2019) causing a human pneumonia outbreak, showing its close genetic relationship to bat coronaviruses and its use of the ACE2 receptor for cell entry.

Immune responses in mice induced by multi-epitope DNA vaccine and protein vaccine of Crimean-Congo Hemorrhagic Fever Virus

This study evaluates immune responses in mice to multi-epitope DNA and protein vaccines against CCHFV, finding that DNA prime-protein boost strategy enhances both cellular and humoral immunity.